EMAGE mouse gene expression spatial database

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EMAGE:31003

4921524J17Rik ( MGI:1913964)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000571_01	euxassay_000571_02	euxassay_000571_03	euxassay_000571_04	euxassay_000571_05

euxassay_000571_06	euxassay_000571_07	euxassay_000571_08	euxassay_000571_09	euxassay_000571_10

euxassay_000571_11	euxassay_000571_12	euxassay_000571_13	euxassay_000571_14	euxassay_000571_15

euxassay_000571_16	euxassay_000571_17	euxassay_000571_18	euxassay_000571_19	euxassay_000571_20

euxassay_000571_21	euxassay_000571_22	euxassay_000571_23	euxassay_000571_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

esophagus

moderate

regional

moderate expression: see section 12

meckel's cartilage

moderate

regional

moderate expression: see section 04 05 06 08 09 16 17 18 weak expression: see section 19 20 21 22 23

vertebral axis musculature

moderate

regional

moderate expression: see section 01 04 05 06 08 09 10 12 13 14 16 17 20 21 22 24 weak expression: see section 02 03 07 18 23

head mesenchyme

moderate

regional

moderate expression: see section 01 02 04 05 06 08 09 10 17 18 19 20 21 22 weak expression: see section 03 07 23

lower jaw incisor

moderate

regional

moderate expression: see section 10 12 13 14 weak expression: see section 15

lung

moderate

regional

moderate expression: see section 04 05 06 08 09 10 14 16 17 18 20 21 22 weak expression: see section 03 07

urethra of male

moderate

regional

moderate expression: see section 13 14

axial skeleton

moderate

regional

moderate expression: see section 10 12 13 14 17

nose

weak

regional

weak expression: see section 08 09 10 12 13 14 15 16 17 18 19 20

otic capsule

strong

regional

strong expression: see section 01 moderate expression: see section 02 22 24 weak expression: see section 03 04 05 06 16 17 18 19 20 21 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1763
Entity Detected:	4921524J17Rik, ( MGI:1913964)
Sequence:	sense strand is shown >T1763 AATGACCAGAAAAGCCCCGGAACCTGGATGGGTCTTTCCCGATTGGAGGGACAGGAGTACGGGTGGAACA CACAGCTGGAGCAGCTGACGTGCTTGCTAGTTTTCAGGTTCGCACCAGAGATGCAGTGTCTTAAGTCCTG TCAGGAGAGTGACTTTTAAATGTATGTATTTCAGGCTTTCACCATTAACAGATTGCATATATACAATCCA TTGACTTTTAATAAATTGGTAATTGATATTTTATTGAATGGAGAATTACGAATATAGATTATAAATTCTG TCTTTAATTGACCTTTTTCTTGGGCGCTATTTTAATGATTTAAGTAGGAGTTATATTTT
Notes:	The probe template was PCR amplified from IMAGE:483649 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:483649 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000571 same experiment
	EMAGE:32185 same embryo
	EMAGE:32184 same embryo
	EMAGE:30399 same embryo
	EMAGE:30578 same embryo
	EMAGE:30983 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source