EMAGE mouse gene expression spatial database

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EMAGE:31008

Bphl ( MGI:1915271)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000541_01	euxassay_000541_02	euxassay_000541_03	euxassay_000541_04	euxassay_000541_05

euxassay_000541_06	euxassay_000541_07	euxassay_000541_08	euxassay_000541_09	euxassay_000541_10

euxassay_000541_11	euxassay_000541_12	euxassay_000541_13	euxassay_000541_14	euxassay_000541_15

euxassay_000541_16	euxassay_000541_17	euxassay_000541_18	euxassay_000541_19	euxassay_000541_20

euxassay_000541_21	euxassay_000541_22	euxassay_000541_23	euxassay_000541_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

choroid invagination

strong

homogeneous

strong expression: see section 04 08 12

choroid fissure

strong

homogeneous

strong expression: see section 08 12 15 17

medulla oblongata part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 04 07 12 13 14 15 16 17 18 19 20

atrioventricular canal

weak

homogeneous

weak expression: see section 03

metencephalon part of 4th ventricle choroid plexus

strong

homogeneous

strong expression: see section 04 08 09 10

trigeminal v ganglion

strong

homogeneous

strong expression: see section 11 moderate expression: see section 10

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 11 weak expression: see section 03 04 05 06 07 10

lateral ventricle choroid plexus

strong

regional

strong expression: see section 04 08 09 10 11 15 16 17 18 19

3rd ventricle choroid plexus

strong

homogeneous

strong expression: see section 04 13

nasal septum

strong

graded

strong expression: see section 11 weak expression: see section 10

nose

weak

graded

weak expression: see section 09 10

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T753
Entity Detected:	Bphl, ( MGI:1915271)
Sequence:	sense strand is shown >T753 ATCTCGAGCCTGTTGGCCTACTGGATGCGCGTTCCTNAAAGCTTGTGCGACCTGGAGCTATGGCGACCGC AACAGTTCGCCCGGCGGCCCAGCGGNTGCGGCTGCTCCTCTCGCCCCTGAAATCCCGGATCTGCGTACCC CAGGCCGAACCCGTAGCCACCTTCGGCACTGCAGTAACTTCTGCCAAGGTGGCTGTGAATGGCGTTCACC TGCATTACCAGCGCGTGGGAGAAGGGGAACATGCGATCCTCCTGCTTCCTGGGATGTTGGGTAGCGGAAA GACCGATTTTGCACCTCAGCTTCAGAGCCTAAATAAGAAGCGCTTCACATTGGTGGCCTGGGACCCTCGA GGATATGGATATTCCAGACCCCCAGATCGAGATTTTCCACGGGATTTTTTTGAAAGGGATGCAAAGGATG CTGTTGACTTGATGAAGGCTCTACAGTTCAAGCAGGTCTCCCTCCTGGGCTGGAGTGATGGCGGCATAAC TGCGCTCATCGCTGCTGCAAAGTACCCATCTTATATCCGCAAGATGGTGATCTGGGGAGCAAATGCCTAT GTCAC
Notes:	The probe template was PCR amplified from IMAGE:1891084 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1891084 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000541 same experiment
	EMAGE:30129 same embryo
	EMAGE:30580 same embryo
	EMAGE:29204 same embryo
	EMAGE:30126 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source