EMAGE mouse gene expression spatial database

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EMAGE:31009

Mmd monocyte to macrophage differentiation-associated ( MGI:1914718)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002968_01	euxassay_002968_02	euxassay_002968_03	euxassay_002968_04	euxassay_002968_05

euxassay_002968_06	euxassay_002968_07	euxassay_002968_08	euxassay_002968_09	euxassay_002968_10

euxassay_002968_11	euxassay_002968_12	euxassay_002968_13	euxassay_002968_14	euxassay_002968_15

euxassay_002968_16	euxassay_002968_17	euxassay_002968_18	euxassay_002968_19	euxassay_002968_20

euxassay_002968_21	euxassay_002968_22	euxassay_002968_23	euxassay_002968_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

strong

homogeneous

strong expression: see section 23 24

head mesenchyme

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 20 21 22 23 24

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 18 19 20 21

stomach

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 13

rib

strong

regional

strong expression: see section 21 22 23 24 moderate expression: see section 03 04 05 06 07 08 20

rectum

strong

regional

strong expression: see section 14 15 16

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 19

retina

strong

homogeneous

strong expression: see section 01 02 03 04

midgut

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21

bladder

strong

regional

strong expression: see section 12 13 15 16

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 12 13 17 18 19

esophagus

strong

regional

strong expression: see section 13 14

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 15 16 17 18 19 20

hindgut

strong

regional

strong expression: see section 12 14 15 16 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4300
Entity Detected:	Mmd, monocyte to macrophage differentiation-associated ( MGI:1914718)
Sequence:	sense strand is shown >T4300 CGGGAGGCGCCGGGGCCGTTTAAGCGGCCTCCCTCCCGCCCCCAGGCGCCTGCTCTGGCCCGGGCCCTGT GCTGACCGACCCCCGGTGTGGCCCACTCCGGCCCTGCCCAGTTGCGTGGCTCCCGCCTGGCACGCCAGCG GCCTCGGAGCAGCTCAAGCCCATGAGGCCGGCGCGCCCTGCCGCCGGTGCAAAAGAGACGGAGCTCCCGG CCCCCGCGGTGGAGCGGTGGATCAATGCGGTTCAGGAATCGATTCCAGCGTTTCATGAACCATCGGGCCC CAGCTAATGGCCGCTACAAACCAACGTGCTACGAACATGCTGCCAATTGCTACACACACGCATTCCTCAT TGTTCCGGCCATTGTGGGCAGTGCCCTCCTCCATCGGCTGTCTGATGACTGCTGGGAGAAGATAACAGCA TGGATCTACGGGATGGGCCTTTGCGCCCTCTTCATAGTCTCCACAGTGTTTCACATAGTATCATGGAAGA AGAGCCACTTGAGAACAGTGGAGCATTGTTTCCACATGTGCGATCGGATGGTCATCTACTTCTTCATTGC TGCTTCCTACGCCCCATGGTTAAATCTCCGTGAACTTGGACCCCTGGCATCTCATATGCGTTGGTTTATC TGGCTCATGGCAGCTGGAGGAACCATTTATGTATTTCTCTACCATGAAAAGTATAAAGTGGTTGAACTTT TCTTCTATCTCACGATGGGATTTTCTCCAGCCTTGGTGGTGACATCAATGAATAACACTGACGGACTTCA GGAGCTCGCCTGTGGGGGATTGATTTACTGCTTGGGAGTTGTGTTCTTCAAGAGCGATGGCATCATTCCG TTCGCCCATGCCATCTGGCACCTGTTTGTGGCCACGGCAGCTGCCGTACACTACTATGCCATTTGGAAAT ACCTGTACCGAAGTCCCACAGACTTTATTCGGCATTTATGACCAGTCTGTGCTAGGTTTTCAAATCAGTA TTATCACGATGGAGGCACTTGGGTGGGGGGAGGGGCGGGGGGAGAACTGAATGTCGCACACAGAAGAAGA GTGAGCAAACTTGCACTGACTTCGTTTTAATTTTTTTTTAATATAATTACTGTGAAAGTATAAAAGCTGT GTCTCGGAGTTTTCCTCTTCACAGGCAACAGATGAGGTAGTGAATCAATTCTTCATTCCATTCCACTATC ATGAAGGACTCGGGAGAGACTTGGCCAACTGATGTTTACAAACCAGAAATTTTTGTATTTTTCTTTTACA GATTTTAACTACGATGATTTTTTTCTAAATTAAGTAGGTCAGGTTGTCAAAGTCAGTGCAATAGTAATAC AACTTCCTTTTTAAGAAAACCCAAAGTTGTTTCTATCACGTTTCCCGTTCACTGTGTAAAAGAATCATGG AAACTGAACACTACTTTTTACCATGTTTCATCTTGACATAACATGGTTATTTTTTAAAAAGGAGGCTTTA GTTCTTTTGTAAATTTTTAAAGAAACGATTCCATTGACGCACACCCCCACTGGCTCCTCATCCATGCGAG GTTTTTGCAGCAAACGGTCAACATTCCACGAACAAACATTATACCTCTTCTGGCAGTTTTATTAAGTGTG GAGAACTTGCCAATTTTTTAGAAACACTGCAGTTTTTTCCAACCTTTTCTGCCAATCTCTAACCCTGAAC TCAGTGCTGCTTTGGGCTGACATCCATGACTGGTGTGGGCCACCAGGAGTAAGACAGTAGTGTTTTGATA CCATCCACTTTTTCAGAAGACATAACTTCGCTACATGCCTTTGCCCCAGTTTTTCTCCAGGGTCTCTCTC CACAGTCGGGGAGGATCCTGTCCGGATTATGATAGTATTATTGGGTTGCCCATCTGCCATGAGCTTTCTG AATCATGGCGAATTGTATTTGCCACTTTGGTGCCCAACGGTTCAAGTCATTGTGGTTAAGCCACTTAACT TTATTGTATGAGTTTGATATTCATTTTAATTGTGGGACTAGACACATAAACTCACATTTCTGCCTTTTCC TCTGCATTTCTCAATATATTATGTTTTTCTCACGCCATAAATAAAACATTGATTGGCAGGTTGAAGCTTG TGTCAGTTGTTGATGGGTTTTATGCTGACTCTGTGGAGTGATATTTATATATTATTTTTAGTTTCCTCTC AATGTCTTATATTAAGATTGATGTATTAGTTGCTTTGTTGGTTAATCTTTCTTGGTGTTTAAGAGAAATG AAGTCACCTTGCCTTTAGATCAGATGCTATTATAACCTGTCTGTCTAGGAACAAGCTGTCAGACTAAAGA TATGGTTTATTCATTGAAATTCTGGACAGCTGTACGTTTAAAAGAAAGTTTATTTTGTATGTGTGTCTAG GCTCTGATTTTTGGTAAATTGTAAAGGAACAACTGAAGAACTGGTTGCTGGGGATGTCTTCAGTAGTCAG GAGTACCCAAGGATAACACCAGCCACACCGCTGTATATGCATGATTCTGAACAGTTAGCCGCCTGTTATT TTACTGTGTATATTTGACTTTAAATATATTAACTTTTTGTGGATTCATTTAAAGTCTGCTCATATGCAGC ACTGTCAAAACCACTAAACTGTATTTGAGAACTTGTGCTGTAGGTTAGAATAAAGTTGTTACTTGGGTTG AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4506710 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4506710 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002968 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source