EMAGE mouse gene expression spatial database

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EMAGE:31013

Mab21l1 mab-21-like 1 (C. elegans) ( MGI:1333773)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002966_01	euxassay_002966_02	euxassay_002966_03	euxassay_002966_04	euxassay_002966_05

euxassay_002966_06	euxassay_002966_07	euxassay_002966_08	euxassay_002966_09	euxassay_002966_10

euxassay_002966_11	euxassay_002966_12	euxassay_002966_13	euxassay_002966_14	euxassay_002966_15

euxassay_002966_16	euxassay_002966_17	euxassay_002966_18	euxassay_002966_19	euxassay_002966_20

euxassay_002966_21	euxassay_002966_22	euxassay_002966_23	euxassay_002966_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vagus x ganglion

moderate

regional

moderate expression: see section 09 19

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 15 16 17 18 19 20

ventral grey horn

weak

regional

weak expression: see section 10 11 12 13 14 15 16 17

pituitary gland

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 09 19 20 21 22 23 24

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4338
Entity Detected:	Mab21l1, mab-21-like 1 (C. elegans) ( MGI:1333773)
Sequence:	sense strand is shown >T4338 GCACACTCTGCACACTTTTCCTCTACCTGATTAACAGTCCTGCACACACCATGATTACGGGAAAACGTAA ATAAATACGGAAAGGGGTGATTATTTTGACTACTGGAAGAGCTTTGCTGGGTCTCAGCATAACTTTTGTT TTTATTACCGAGAAGGTGCTCTCTCCACGCGTCTCTCTCTGACACGGATTCTTTAGCGGAGGGGAGAAAA GGGGAGAGCTCAGCCTTTCACTTTAAGACCCGCTAGTCTCGAGCGGGGAAAAAAAGGCCAGCAGGGCAGG GCGGCCCCCTGCTGCAGCTAGTCCGAGAGAGGCTCGGTGCCTTTTGAGCGTGGTCCCCTTTCTCCACCGA AGTTGGCTCCAGCGCTAGCAGCCGCATTGGATCCCACAGCCTATTGCGAGACTCCGGTGTACAATCCGGA TCTCTGCCCCAACATGATCGCGGCCCAGGCCAAGCTGGTCTACCACCTGAATAAGTACTACAACGAGAAA TGCCAGGCCAGGAAAGCTGCCATCGCCAAAACCATCCGGGAAGTCTGCAAAGTGGTCTCCGACGTCCTGA AGGAGGTTGAAGTGCAGGAACCGCGTTTCATCAGCTCCCTCAATGAGATGGACAACCGCTACGAGGGCCT GGAGGTTATCTCTCCCACCGAGTTCGAGGTGGTACTCTATCTGAACCAGATGGGGGTGTTTAACTTCGTG GACGACGGCTCCCTGCCTGGCTGTGCGGTGCTGAAACTGAGCGATGGGCGCAAGAGGAGCATGTCCCTCT GGGTGGAATTCATCACCGCCTCTGGCTACCTCTCGGCACGCAAAATCCGTTCCAGGTTTCAGACACTGGT GGCTCAGGCGGTGGACAAGTGTAGCTACAGAGATGTAGTAAAGATGGTGGCTGACACGAGCGAAGTGAAA CTGAGAATCCGAGATAGGTATGTGGTGCAGATCACCCCGGCCTTTAAATGCACCGGGATTTGGCCGCGGA GTGCTGCCCACTGGCCGCTTCCCCACATCCCCTGGCCAGGACCCAACCGGGTGGCAGAGGTTAAGGCTGA AGGGTTCAATTTGTTGTCCAAGGAGTGCCACTCCCTGGCCGGCAAGCAGAGTTCGGCTGAGAGCGATGCT TGGGTGCTGCAGTTTGCAGAGGCCGAGAACAGACTGCAGATGGGGGGCTGCAGGAAGAAATGCCTCTCTA TCCTGAAAACCCTGCGTGATCGCCACCTGGAGTTGCCAGGCCAGCCCCTGAACAACTACCACATGAAGAC TCTGGTGTCCTACGAGTGCGAGAAGCATCCTCGAGAGTCGGACTGGGACGAATCTTGCCTGGGCGATCGG CTGAACGGAATTCTTCTGCAGCTCATCTCATGCCTGCAGTGCCGGCGGTGTCCCCATTACTTCTTACCGA ACTTAGATCTGTTTCAAGGCAAACCCCACTCGGCCTTGGAAAACGCTGCTAAACAAACGTGGCGACTGGC AAGAGAGATCCTGACCAACCCAAAAAGTTTGGAAAAACTTTAGAGGATGATCTAATCCAGGGCTGACAAG AATGAGCATTCTTCTCAAAGTCCCGACGAAATGAAAACTTCCTGGTTGATTCCTATGTACTATTTCTCGA GACAGTGCAAACAATCTCTTCCTTTAAAAGGAGTAACAACACAACATTTAACCATCATCTCACACAGGGG AAAAACCACACCACAAGCATCTTTATGGAGGCATCCCCCAGCTCCCCCGAAAAGTGCACAACACCCCAAA ACAGCTGGCTGCAATGCAAGACCATGGCCAACTCCAGCAAAGGATTATAGTAATCTTGAGACTTAGGTGC GGCTGCCTTCCAGCAAACAGGGCCCGGATGTACCATCGGAAGCAAAGTGTAATGTATATTTTGATCTGTA ACAAAAGTTGTGATCTCATGTTGTCTTTGAAAGTGTGGATATTGGTGTTTTGTGATTTGGTGATCAGAAC TGAAATTACCATTTTGGAAACTTTCAGACATTTCCCACAGAACAGAGATGCATTTAAAGGTAGATTTCCT CCTGGTACTTTACCTGTCTTTCAAGTGTCTGAACTTAAAACAAAAGTTTACATTTTGTTTCAAATATATT GCTTGTTCTAACATTCCGTAAGTATACTTGAAATGTTATTTAAATATATTCAAAAATTTGAATTCAGCTT ATATAATAACGCTTGAATATCTGAATTATATATATATTTGAAAATGCACTTGAAAATACACTGGATAATT ACTTTTGTGATTTAGAATTTAATTTCTGTTGCTGATTTTTATTTAATTAGAAGATAATAAATGAAGTAAA ATACCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4526962 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4526962 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002966 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source