EMAGE mouse gene expression spatial database

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EMAGE:31014

Chrna6 cholinergic receptor, nicotinic, alpha polypeptide 6 ( MGI:106213)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002965_01	euxassay_002965_02	euxassay_002965_03	euxassay_002965_04	euxassay_002965_05

euxassay_002965_06	euxassay_002965_07	euxassay_002965_08	euxassay_002965_09	euxassay_002965_10

euxassay_002965_11	euxassay_002965_12	euxassay_002965_13	euxassay_002965_14	euxassay_002965_15

euxassay_002965_16	euxassay_002965_17	euxassay_002965_18	euxassay_002965_19	euxassay_002965_20

euxassay_002965_21	euxassay_002965_22	euxassay_002965_23	euxassay_002965_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

midbrain mantle layer

strong

regional

strong expression: see section 11 12 13 14 15 16

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 moderate expression: see section 09 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4216
Entity Detected:	Chrna6, cholinergic receptor, nicotinic, alpha polypeptide 6 ( MGI:106213)
Sequence:	sense strand is shown >T4216 GACAGGACATGTGACAGCCAGCCGAGTGAAGCAGCAGGGTTGGCTTCCCTTGCCCTTCACATCTGTTTCA GCCTGGGATGCAGGGAGCAGTTGCATGATGTCCCGTGTTTTCACTTCACAGAAGATCTGTGTGAGCTTCC AGGAGCTTCCCGGGGCTTTCAATATCTGATTCTGGCAGCTGACAGACAGCTGTGTCTTCCACAGCCCTCC GTGTAGAAATCGGAATTCAGAGAGCTTTTATCATGCTGAACAGCCGGGACCAGGGAAACCTGCACTCCGG TTTATGTCTGTGGCTATGTGGATTCCTGGCTCTCTTTAAAGGCAGTACAGGCTGTGAATCTGAAGAGCAG CTCTTCCACAGGCTGTTTGCTCACTACAACCGCTTCATCCGGCCGGTGGAGAATGTCTCCGATCCCGTCA CGGTGCATTTTGAATTGGCAATCACGCAACTGGCCAATGTGGATGAAGTCAACCAGATTATGGAGACCAA TCTGTGGCTGCGTCACATCTGGAAGGACTACAGATTGCGTTGGGATCCAACGGAGTATGATGGCATCGAG ACACTTCGAGTTCCAGCAGACAACATCTGGAAGCCTGACATCGTTCTGTATAACAATGCTGTCGGCGACT TCCAGGTCGAAGGCAAGACCAAAGCTCTTCTCAAGTATGACGGTGTGATAACCTGGACCCCACCAGCCAT CTTTAAGAGCTCCTGCCCAATGGACATCACCTTCTTCCCGTTTGACCATCAAAACTGCTCCCTGAAGTTT GGTTCCTGGACTTATGACAAGGCAGAAATCGACCTTCTCATCATTGGCTCTAAAGTAGACATGAACGACT TTTGGGAAAACAGTGAATGGGAAATTGTCGACGCCTCTGGCTATAAGCATGACATCAAGTACAACTGCTG TGAAGAGATTTACACGGACATAACCTACTCCTTCTACATCAGGAGGTTGCCCATGTTTTACACCATCAAC CTCATCATCCCCTGCCTCTTCATTTCCTTCCTCACGGTGCTGGTTTTTTACCTTCCCTCCGACTGTGGCG AGAAAGTGACTCTTTGCATCTCCGTTCTGCTTTCTCTCACTGTCTTTTTGCTGGTGATTACAGAGACCAT CCCATCCACATCTCTCGTGATCCCACTGGTGGGTGAGTATCTACTGTTCACCATGATCTTTGTCACGCTG TCCATTGTGGTGACCGTGTTCGTGCTGAACATACACTACAGGACCCCAGCAACGCATACCATGCCCAAGT GGGTGAAGACCATCTTCCTTCAGGCCTTCCCCTCGATTCTGATGATGAGGAAACCTCTGGACAAGACAAA GGAGGCAGGAGGTGTTAAGGACCCCAAAAGCCATACCAAGAGGCCTGCCAAGGTCAAATTTACTCATCGA GGAGAATCCAAACTTCTAAAGGAATGCCACCACTGCCAAAAATCAAGTGACATAGCACCTGGAAAGAGAA GATCAAGCCAGCAGCCTGCACGGTGGGTGGCAGAGAATTCAGAGCACTCGTCCGATGTTGAAGATGTCAT CGAGAGTGTTCAATTCATAGCAGAAAACATGAAGAGCCACAATGAAACAAACGAGGTAGAAGACGACTGG AAATACATGGCTATGGTGGTGGACAGAGTCTTCCTTTGGGTATTTATAATTGTCTGTGTGTTTGGAACTG TGGGGCTATTTCTGCAGCCACTGCTTGGGAACACAGGAAAGTCTTAATTGGTATTGTCCCTTTACCTTCT GAATCTTTTAGATGTTGTGTTTTTTCCCGAGCTAGTCCCCACAAGAAAAAGCCAAGTACACAGACTCCTA AACCAAGACAAGAAGGGAGTGGGCATTGCTGCAGCCTTAGGAGCCTGAATACCTGTCTTCCTTACACTGG GTGTTTTATCACCTTGGAGCCCAGAGCTGGCAGTTGTCCTAGGCTAGGAGGACTGAGTCAGCTTCTCTAT AATTTGCATTCTTTGTTTCAAGAAACACAAATGGTCCCTCGTAGAGCTAGGCCCCATAGTCAAGAGTCAG TCCACTCTGAAAGGTGATTGGCTTGTATCCGAAGCTCCTGCTGGTTATTGCAAGTGATGTGTATGGTTAT CAAGAAACTCGGGAGCGCTGCTGACACTTGCAACACACCTGTCTGCTACTTTCTTGTGCATGTCCCTGTG ATGTAGCTCACACTCATATCTGACATGTGCAATGGTCAAAGAAGAAAGCAGATAGCATCTCTCTTAGCAG ACTATTCAATTCGTGGTGCAATGGGTTTTCTGTCCGTGTGAAAATCACTGAACTGATAGGCGTAAAAGGA TGACGTTTATATGAGCTCATGGATTTGGAGGACTCTGCCCATGATCACTAGGTCCCATTGCCTCTGGGCC TTTGATGAGGCAAAGCATCATGCCTAGAAGAGGATGGCTGCCCACTTAAAGATAGCTACAAAGAAAGCAA ACAGAGAGGAAGGCTCTGGAGTGCCAATATTCCTTTCACAGTCACAGCCCAATGACTTCATGTCTTCCTA CAAGACCTTCCATCCTAGAAGCTCCACTACCTCCTGATAGCGTGGAGGAATCAGTCTCTAACACTTGTCC TTGGGGCTGATCCAAACCACAGCAGTATTGTCCTGGCAGGACATTTGGCTGGCTCCAGTACTGTGCTAGG TGCTACAAGGGGATACATAAGAGTCTTTCCCTTATAAAGTTACAGCCTTTTAGAAAAGCCGTATATGCAA AGAGCCACAGGATCAGGTAGTAGAGGATATAGCTAGAGCAACCCAAATGGGAGAAACTAATCGGACAAAC CTAAACTCACTCTTGTGTTTCAAACAGATATCTGTGTAGCTTTTTCCCACGAGCCCTTCTCTAGCTCACT GCATGGAGATGAAATAAAATCGATATGAAATTTTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4501558 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4501558 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002965 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source