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EMAGE:31019

1700025G04Rik ( MGI:1916649)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31019 EMAGE:31019 EMAGE:31019 EMAGE:31019 EMAGE:31019
euxassay_004907_01 euxassay_004907_02 euxassay_004907_03 euxassay_004907_04 euxassay_004907_05
EMAGE:31019 EMAGE:31019 EMAGE:31019 EMAGE:31019 EMAGE:31019
euxassay_004907_06 euxassay_004907_07 euxassay_004907_08 euxassay_004907_09 euxassay_004907_10
EMAGE:31019 EMAGE:31019 EMAGE:31019 EMAGE:31019 EMAGE:31019
euxassay_004907_11 euxassay_004907_12 euxassay_004907_13 euxassay_004907_14 euxassay_004907_15
EMAGE:31019 EMAGE:31019 EMAGE:31019 EMAGE:31019 EMAGE:31019
euxassay_004907_16 euxassay_004907_17 euxassay_004907_18 euxassay_004907_19 euxassay_004907_20
EMAGE:31019 EMAGE:31019 EMAGE:31019
euxassay_004907_21 euxassay_004907_22 euxassay_004907_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
sternum
strong strong
regionalstrong expression: see section 11 moderate expression: see section 08 09 10 12 13
pituitary gland
moderate moderate
regionalmoderate expression: see section 10 11 12 13
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 16 moderate expression: see section 07 08 17 18
stomach
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07
metanephros
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 13 14 16
spinal cord
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13
brain
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 07 16 17 18
retina
moderate moderate
regionalmoderate expression: see section 01 02 03 20 21 22 23
midgut
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16
otic capsule
moderate moderate
regionalmoderate expression: see section 06 07 08 09 15 16 17 19
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 07 08 12 13 14 15
pharyngo-tympanic tube
moderate moderate
regionalmoderate expression: see section 01 02
thymus primordium
strong strong
regionalstrong expression: see section 10 11 12 13
trigeminal v ganglion
strong strong
regionalstrong expression: see section 20 moderate expression: see section 03 04 05 06 07 08 16 17 18 19
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 10 11 13 14 15
adrenal gland
strong strong
regionalstrong expression: see section 06 07 13 14 15
vibrissa
strong strong
regionalstrong expression: see section 04 05 06 20 moderate expression: see section 07 18 19
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 08 09 10 11 13 15 16 17
tail
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15
limb
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 16 17 18 19 20 21
lung
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6927
Entity Detected:1700025G04Rik, ( MGI:1916649)
Sequence:sense strand is shown

>T6927
GGGTTTTCGGCTTTTACTCCAGGCGGCGGGGAGGAGGGGGAGCCCCGGCCACACTGTAGGGAGGAGGAAG
AGGAGGACGTGGAGGAGGGAGGAAGAGAAGAGGCGAGGAGGCCAGGGGCGGGGGGCGGGGGGCTTGCCAC
CTTCAGCCCCCCCCCGCGAGCGCCCAAGGTGTACACACTGTTACCAGCACAGCCACAAGGCGGATTTCCT
GTGTGCTTGAAGAGAGAGAAAGCAATGTCCCCGTGTCTGTAGAGATGATTTGCAGTCCAGCCCTGCTGAA
GCTGACCGAATGAGACTATGGGCTGTGCCTCTGCCAAGCATGTCGCTACTGTTCAAAATGAAGAGGAAGC
ACAGAGAGGGAAAAGCTACCAGAATGGAGATGTGTTTGGCGATGAGTATAGGATCAAACCCGTGGAAGAG
GTCAAATACATGAAAAATGGGGCAGAAGAGGAGCAGAAAATAGCAGCCAGAAACCAAGAAAACCTGGAAA
AAAGTGCCAGCTCGAACACAAGGCTTAAAACTAATAAAGAAATCCCTGGATTAGTTCATCAACCCAGAGC
AAACATGCACATCTCTGAAAGCCAACAAGAATTCTTCAGAATGCTGGATGAAAAAATCGAAAAGGGTCGG
GATTACTGTTCTGAAGAAGAGGATATCACATAGCATCGGTTCTCAGTCGGACCAGGAGCTACTACTGTGT
AAATAGGTTGCACCCCAGTTGAAACCTTTGCAAAGGTCGGTTCTCTTCACTGAACAGCACTATAGCGAAC
GAAGACTGTTCCATGTCCTATGCCCCATTAAATTACAGTGTTTCTTAATGAACCTGCAGAGGAATATTGC
TAAACACAAACACACACACACACACAAGAACAAAAACAACAACAACAACAAAAAAACTGTTCTGGGACTT
TCTTTGTTGCTGCTAGTTAAAACTTGTTGCAACTCTTCACTTCTCTCGTGTCCAAGTATGCAGCAAAATT
CTGCAGTTCACTTTAAAGATGTTATTGGTTTCAGGATGCTCTCTAGCCTGTATCCTCGGCGATTGGGTAG
AGGTGAACTCTGATACAGATGTCAGTTCTAGCCTGGTTCTGCTTTGATGTCAAGCATCCCCTCCCTTCTA
TTCTCCTTATCCATCTCTTTCGAGTGGTCCAGAGATGGCCTGAGCCTTGCTTCTCACTGGTAACGTGGGG
CTTTGGACATGGGATGGTGGCCACGGTGAGCCTAGTTTCTCTGCTTGAAAGAGCCACAATTGATTGAAAG
CCTGTTGTAACCTAACCTGACTCCCGGAAGTGACAAAGGAACAAGTGAGCAGTAGGTTGTTCCGCTTACT
AGGACTCTTAGTGCAGGATTAAGCTTGTAGCATTGGCTTTTGCTCAGATGCAAATGGAAGTGTGACCACG
TGACCACAATCATTTTCAACCCCTCCCTCCTCACTTTTTTTTCTAAGAAAATAAACATTTTGCTGTTTTT
CTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3981517 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3981517 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_004907 same experiment
 EMAGE:30956 same embryo
 EMAGE:30118 same embryo
 EMAGE:30448 same embryo
 EMAGE:31157 same embryo
 EMAGE:30133 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS