EMAGE mouse gene expression spatial database

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EMAGE:31033

Th tyrosine hydroxylase ( MGI:98735)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007178_01	euxassay_007178_02	euxassay_007178_03	euxassay_007178_04	euxassay_007178_05

euxassay_007178_06	euxassay_007178_07	euxassay_007178_08	euxassay_007178_09	euxassay_007178_10

euxassay_007178_11	euxassay_007178_12	euxassay_007178_13	euxassay_007178_14	euxassay_007178_15

euxassay_007178_16	euxassay_007178_17	euxassay_007178_18	euxassay_007178_19	euxassay_007178_20

euxassay_007178_21	euxassay_007178_22	euxassay_007178_23	euxassay_007178_24	euxassay_007178_25

euxassay_007178_26	euxassay_007178_27	euxassay_007178_28

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

metencephalon

strong

single cell

strong expression: see section 08 09 10 11 12 13 22 23 24 25 26 moderate expression: see section 07 20 21

medulla oblongata

strong

single cell

strong expression: see section 09 10 11 12 13 14 15 17 22 23 24 26 moderate expression: see section 20 21

cervico-thoracic ganglion

strong

regional

strong expression: see section 14 15 16 17 24 25

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 09 10 11 26 27 moderate expression: see section 28

thoracic ganglion

strong

regional

strong expression: see section 17 18 19 20 21 22 23 24

dorsal root ganglion

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19 24 25 26 27 28

esophagus

strong

regional

strong expression: see section 18 19 20

olfactory lobe

strong

single cell

strong expression: see section 11 13 14 15 16 19 20 21 22 23 24

vagus x ganglion

moderate

regional

moderate expression: see section 10 11 26

heart

moderate

spotted

moderate expression: see section 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 23 24 25 26 27 28

spinal cord floor plate

strong

regional

strong expression: see section 18 19 23

adrenal gland

strong

regional

strong expression: see section 13 14 15 16 24 25 26 27 28

telencephalon ventricular layer

strong

single cell

strong expression: see section 12

cervical ganglion

strong

regional

strong expression: see section 12 13 23 24 25

telencephalon mantle layer

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28

midbrain mantle layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19 20 23 24 25 moderate expression: see section 22 26

diencephalon lateral wall mantle layer

strong

single cell

strong expression: see section 10 11 12 13 14 15 18 19 20 moderate expression: see section 08 09 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50020
Entity Detected:	Th, tyrosine hydroxylase ( MGI:98735)
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. .The reverse primer contains a 5' extension containing . T7 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template usi. T7 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007178 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source