EMAGE mouse gene expression spatial database

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EMAGE:31046

Lysmd4 LysM, putative peptidoglycan-binding, domain containing 4 ( MGI:1922349)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002979_01	euxassay_002979_02	euxassay_002979_03	euxassay_002979_04	euxassay_002979_05

euxassay_002979_06	euxassay_002979_07	euxassay_002979_08	euxassay_002979_09	euxassay_002979_10

euxassay_002979_11	euxassay_002979_12	euxassay_002979_13	euxassay_002979_14	euxassay_002979_15

euxassay_002979_16	euxassay_002979_17	euxassay_002979_18	euxassay_002979_19	euxassay_002979_20

euxassay_002979_21	euxassay_002979_22	euxassay_002979_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 17 18 weak expression: see section 08 09 10 15 16

facial vii ganglion

weak

regional

weak expression: see section 04 05 20 21

vagus x ganglion

weak

regional

weak expression: see section 08 09 17

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 18 19

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4264
Entity Detected:	Lysmd4, LysM, putative peptidoglycan-binding, domain containing 4 ( MGI:1922349)
Sequence:	sense strand is shown >T4264 GAGCGAGTTAAGAGAAAATGAGGCAGAAGGAAGTGTTAGCCAAGAGCTTCCAAGGTCCAGCTGCTGTCTG CAGGACTCCAAACAGCCACGTTTACATGTTCAACAATGGCAGCGGGGACTCTGGGGACTCCTCTGAGGAA GAGTCACATCAGGTGGTGTTACGACCCCGGGGCAAGGAGCACCAGAAGAACAGCAGCCAACGGCCAGGAG CAGGCACCATGGTTCTGCTGCAGCGGGAGCTGGCTCAGGAAGACAGCCTCAACAAGCTGGCTCTCCAGTA TGGCTGCAAACACTCAGAGTGATTAACAGTGGCAAAGTTGCAGATATCAAGAAAGCAAACAACTTCATCA GAGAGCAAGACCTGTATGCTTTGAAGTCAATCAAGATTCCAGTGAGAAATCATGGGATTCTCACGGAGAC CCACCAAGAACTAATGCCCCTTGGGGCTTCATCCTCTGAGACCAGGGTGACCCTGGTAGACCTGCCTGAA GATGAAGATGCTGGTGGAGCAACCACTCAGGGCAACCAGCTGACAGACTTCTTCAAGGGCATTGATGAGA ACATTGAGCGGGCTGTGCATTCTGACGTCTTCCACGGTGACAGCTGCTGTGTGGAGGCCCCAGATCAGCT GCTGCTCCCCATAACCCAGAAGCCAGTAGCCGATGGTGCCGACTGTGGGATTCAGTGGTGGAATGCCGTC TTCCTTATGCTTCTCATTGGGATCGTATTGCCAGTGTTCTATCTCGTCTACTTCAAAATACAGGCCACCG GGGAGCCCTCAAATGGCTTGAACGCAACTGTTGTCCCCAATGGCTCGATGACACTGAGCCCAGTTCCAGG GCAGGCTCCCAGGTTGGCAATCCCAGTGCCTACCCTCCCCGCTTCAGACAGCCAGGTCAGCCCTACCACC CAGGCAGGGGCCTAAGCGCATTGTTTCCTAGAGTTGGCAGACTTTGGCAGTTGGCCCTTGTGTAACTGTC ACCAGCTGTTTGCATTTCGCTTCCTGAGATGTGTGGACACCATGGCAATCTGGATGGTCTAGACTGAGGA ATAGCTTGGCCTAACTCAAGGATGCAGGAGCCCAGACATCCACCATCCCAGCAAGAGAGACATTTATATG AAACTCACAGCTCTTGAAGACATGTAGGGGCCGGCTGTGAGTTCCTGTGCAGCACTCTGTGCAGGCTCCT AAGTAGAATGTGACAAAGGTTCTGCACTATTGCTGCCCCCAGTGTTGAATAGGGTTTCAATGGTGGGCTC AGAGACATGCTCTGTGTCTAGATCAAGGTGCTATATAGTATGTCACTGAGTTTGTCAGTTGACAGCCTGA CATGTTATGTTTGCCTCTTCACTTGGCCCCACTGAGGCAGTGCTTCAGAAATGAGAACCTGAAATCCAAA CCGCCACCCTCTAATTTAAGCTCTACCCCCAGAGTCACCCTAAGAGCATCTCTGTGGTGGTGGCTCCCTG GGTTCCCCAGTTGGCATCTGGGAAGCAGCTGGTATTGGCGAATGGTTTGAAGGGACTACATCCACCATTA AGCTTCTGTCACATGGACCTTTGAAGCAAACTGGTTGCCATGCCAGCCCAGCATTGTTAATAACAGTGAG TCCCACCCCACCCTGATCATGTGCGCCCAAAGCAGAGTTCTCCCACTGCCTCCTTGACTCATCTATTCTT TGGGTCCTTAGAGTAAACTTGCACATGCTGTGTCTCAGTGTGAAAACCAGACACAATGTCAGTCAGCCGT CTTTTACTGTAACAAAATCATCTCCAGTGGGAAAGGTTTATCTTGGCTCATGGTTTCAATCCATGGCCTG ATTTGGGGCTGGGTGGCTGGGGGCTATGGTAAGAACACCATGAAGGAGAGTGTGGTAAAGCAGATTGTTC ATGTGGCAGGGAGAGAGAAGGGGAGAGGAGCTTGGGCGCAGGCATCACCTTCAGAGTTCTGCCTGCAGTG GCCTGACTTCCTCCCACTGGGTCCCATTTCTTAAAGGACCCATTCCTTCCCAACACTGAGATGAGGACCA AGCCTTTAAGATACTATCCAAACCCCACCAAGGTAACTCTGGCTCCGAAAGGCTGGAGAGAGAGATGTAG GAGGGTACAAAATGCTCAGGGTTTCCTCAGCCACTCCCTGAGAGCAGTTGGCCATCAGTCCTGTTAGGGA TGTTGGGGGGAGGGCTAAGTCAGCCTGCCTTGTCACAGCAGGGGCAGGCAGAGGTGGCTGGGAGCTCAGC CTGGGGAGGACAGGCTAGCAGGCTTCTGGGATGGTTAGCAACTTGACACATAGGCCTAGAAATCTGACCA GAGGAGCAGGACCTTACTTGAGCTGGGCCTCCTGTCTGAAAAACAACCCACCCAGGGTGCAGATAGCAAA TTTAAATCATCTTCAGAATTGAGTATTTTGAGGCAGAAAATGAACCCATGAATCTTGGTACAATAAACAT CTTGATTATGTAAGTGATTTCTATCTGGATGCTCAAAGACCGTTGTGGCTGTGAAGTTTCTCTTAAATGT TATCATTAACTTCATCAGGCAGGGCTCAGAGTCCCGTAGGGAAATGATTCTTTTGGCAGACAGAGAATGT CTATATTTTTTCTATCTTCTGTCTCAAGTGTTGGGGACTTATTACCACCTGTATATAGCCCTGCGTGGGT TCCTAAGTAAAAGGTGACAAGGGATCTGCAGTGTCAGGGCTCCTGAGGTTGGATGAGGTCAAATGACTAT CACAGTGTCCCTGCCTGCTGAGGGCAGGGGTGGGCTTGGCTCAGAGATTTGTGTTCTGTGTCTAGGCAGA CCATGGTGCTATGCAATAGTATCAGTATGTCACTGAGTTTTAGACATGTTACAGTTTTAACTTGTATTTC TGTATTTTTCTCAAGTGGAATAAAACAATTTCTTCTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4504057 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4504057 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002979 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source