EMAGE mouse gene expression spatial database

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EMAGE:31050

Pla2g5 phospholipase A2, group V ( MGI:101899)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002977_01	euxassay_002977_02	euxassay_002977_03	euxassay_002977_04	euxassay_002977_05

euxassay_002977_06	euxassay_002977_07	euxassay_002977_08	euxassay_002977_09	euxassay_002977_10

euxassay_002977_11	euxassay_002977_12	euxassay_002977_13	euxassay_002977_14	euxassay_002977_15

euxassay_002977_16	euxassay_002977_17	euxassay_002977_18	euxassay_002977_19	euxassay_002977_20

euxassay_002977_21	euxassay_002977_22	euxassay_002977_23	euxassay_002977_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

metencephalon basal plate

strong

regional

strong expression: see section 08 19

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 21 weak expression: see section 08 09 19 20

testis

strong

regional

strong expression: see section 06 07 08 09 10 11 21 22 moderate expression: see section 23 weak expression: see section 24

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 22 23

vagus x ganglion

strong

regional

strong expression: see section 10 weak expression: see section 19

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 19 20 21 22 23 24

telencephalon ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 17 18 19 20 21 22 23 24

midbrain ventricular layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18

telencephalon marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 18 19 20 21 22 23 24

midbrain mantle layer

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 moderate expression: see section 07 08 09 10 19 20

olfactory cortex ventricular layer

strong

regional

strong expression: see section 11 12 17 18 19

ventral grey horn

strong

single cell

strong expression: see section 13 14 15 16 17 18

olfactory cortex marginal layer

strong

regional

strong expression: see section 11 12 13 14 17 18 19

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18

pons ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 moderate expression: see section 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 09 20 21

pons mantle layer

strong

regional

strong expression: see section 09 10 11 16 17 18 19

hypothalamus mantle layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 13 17 18 19 20

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 09 10 11 12 13 15 16 17 18 19

hypothalamus ventricular layer

strong

regional

strong expression: see section 12 13 14 15

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4234
Entity Detected:	Pla2g5, phospholipase A2, group V ( MGI:101899)
Sequence:	sense strand is shown >T4234 ACTTTCTGCCACCATGAAGGCTGCTGGGGGCTGAGCGGGATGCTTGCTGGCCAGAGTTCATTGCGTGCTC CCATGATCAGAGTCTGTCCTCCTGTGTTGCAGCTTGTAGACCCTGATGCTTGTGCTGCCGGCCATAAACG CAGGGATCCAGACTCTGTCTACTGAGGCTGCTCCTAGGAACGGGCCCTGGGAACAGGCGCTGAGACCAGG CTACAAAGAACCCAAGAGATGAAGGGTCTCCTCACACTGGCTTGGTTCCTGGCTTGCAGTGTGCCTGCAG TCCCAGGGGGCTTGCTAGAACTCAAGTCCATGATTGAGAAGGTGACCAGGAAGAATGCCTTTAAGAACTA TGGCTTCTACGGCTGCTACTGTGGCTGGGGCGGTCGCGGGACACCCAAGGATGGCACTGATTGGTGCTGT CAGATGCACGACCGTTGTTATGGGCAACTGGAGGAAAAAGACTGTGCCATTCGGACCCAGTCCTATGACT ACAGATACACAAATGGCCTAGTCATCTGCGAACACGACTCCTTCTGTCCAATGAGGCTCTGTGCCTGTGA CCGGAAGCTGGTCTACTGCCTGCGGAGAAACCTCTGGACTTACAACCCTCTTTACCAGTATTACCCCAAC TTCCTCTGCTAATGTCCGCTGTGCGCTCTGCCCTGGGGGCACCTCCCACGGTGGCAGTCTCCCTCTGCTG TATTCCTGATGTGTCCCGCCTCCCCCACAAGGTCTTGGATCTGCCTTCCTCTGTGCACCAGTGGACTGGA CAGAGCCCCCCCCCCCCCCCAGGGCTACACTCTTCCCTCCAGAGTCCGAGGAAGGGACTTTGATCTAGAG TTGGCAGACTCTCAGGATGGCTGAGCCTGCACTTGCTAAGTTGGCTCTGGGCCCTGAAGCTCCCCCGGCT CAGGCCAGCCGTGTTGACTTTTCTTTCAATTTCTGGAACCCAACTGTCATTAGCACCCTCCAGAGACCTT TTACTGGAGGAGAAGCCAAATTAACTCTATAAATCTGCTATGTAGATATTAAATAAAACCCATTCTCAAG GCTAGAAAAACACCACCCCAGCACTTCCTCTGACGGGGCTGGGGTGGGATTGCCCACGCTTTTCCAACCC CCCCCCCCCCCCGAGGCATCTGTATGCTCTCGGGGATGGAGGTCAGGAAACAGGTGGGAGCCTTGACGCC TTGATGCCTTTCATGGTTTGTCTTGAGTTCATTTTCTTCAACCTTAGGCTCCTTCAAGCCAGACCTGGGG TTTATGTTTCTTCTGGAGGAAGATGAGACACAGCCCTATGCCACCTTGAGCTCCTGGCTAGAGAGGGCAA CAATCAGCTCCCAGCCCTGGCTTCTGGCAGCTGTATGGTCTTTTAAACCTTTGGATAGTCCGAATCTCTC TGGCTCTCCTCAAAATATAAAACAAGGCTCCTTCCAGGAGTATACTGGTGCACACCCCAATCCCAGCACC TGGAAGGTGGAGGCAAGAGAATCGGGAGTTCAAGGCCAGCCTCTGCTCCCTAGCAGGGATAGTAGCCTAG ACTATGTGAGACTGTGTTTCAGAAAGGACCCTGGTACAGATACAGGGGAGCTGGGGTTCTGAGATGTCAC TCAGTGAGAAAAATGGTTTAAATAAGATTCGGGGGGGGGGGGTAGAGATATATCAATGGCGGAATGTCTG TCTATTTTTCACCGAGTTAGGGGCTCTGAAAATCTCAAGTCATCTATCGCTTTATAAAAGACAGAACTTT CTCATAGCAACAAGACAGGAGGAAGAGATGTATTCAACAGTAAAGCAGTGTCCTTCCGGAGGAAGGGTCT AGGCTTCTTCAAATGGAGCTCCAGAATGTGCACGGGACACATCCGATCTATATTTAGAGACCTTTAGTGT TCAAACATTTTCTAAATAAAGCTAATTATATGAGACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4502326 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4502326 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002977 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source