EMAGE mouse gene expression spatial database

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EMAGE:31067

Pcsk9 proprotein convertase subtilisin/kexin type 9 ( MGI:2140260)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002970_01	euxassay_002970_02	euxassay_002970_03	euxassay_002970_04	euxassay_002970_05

euxassay_002970_06	euxassay_002970_07	euxassay_002970_08	euxassay_002970_09	euxassay_002970_10

euxassay_002970_11	euxassay_002970_12	euxassay_002970_13	euxassay_002970_14	euxassay_002970_15

euxassay_002970_16	euxassay_002970_17	euxassay_002970_18	euxassay_002970_19	euxassay_002970_20

euxassay_002970_21	euxassay_002970_22	euxassay_002970_23	euxassay_002970_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

weak

regional

weak expression: see section 10 11 12 13

hindgut

strong

regional

strong expression: see section 15 16 17 18 19

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 17 18

stomach

moderate

regional

moderate expression: see section 10 11 weak expression: see section 07 08 09

upper jaw incisor

moderate

regional

moderate expression: see section 13 17 18

kidney calyx

moderate

regional

moderate expression: see section 09 10 11 12 19 20 21 22

rectum

strong

regional

strong expression: see section 15 16 not examined expression: see section 17

rest of cerebellum marginal layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midgut

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4222
Entity Detected:	Pcsk9, proprotein convertase subtilisin/kexin type 9 ( MGI:2140260)
Sequence:	sense strand is shown >T4222 CCACGCGTCCGGGAGTGGGGATTAAGAGGGGGGAATGTAACAGGTCCCGTTTGCAGCCCAATTAGGATTT GGGGTTTTGTCCTCACTCTGAGCGTCATTTGACGCTGTCTGGGGAGGGCGAGGCCGAAACCTGATCCTTT AGTACCGGGGCCCGTTAATGTTTAATCAGAGAGGATCTTCCGATGGGGCTCGGGGTGGCGTGATCTCCCG GCCCCCAGGCGTCCAGTACCCACACCCCAGAAGGCTTCCACCTTCACGTGGACGCGCAGGCTGCCGGTGG GCTCCCGTTCTCTCTCTCTTTCTGAGGCTAGAGGACTGAGCCAGTCCTTGGCTCCCCAGAGACATCACGG CCCGCAGCCCCGGAGCCAAGTGCCCCGAGTCCCAGGCGTCCATGTCCTTCCCGAGGCCGCGCGCACCTCT CCTCGCCCCGATGGGCACCCACTGCTCTGCGTGGCTGCGGTGGCCGCTGTTGCCGCTGTTGCCGCCGCTG CTGCTGCTGTTGCTGCTACTGTGCCCCACCGGCGCTGGTGCCCAGGACGAGGATGGAGATTATGAAGAGC TGATGCTCGCCCTCCCGTCCCAGGAGGATGGCCTGGCTGATGAGGCCGCACATGTGGCCACCGCCACCTT CCGCCGTTGCTCCAAGGAGGCCTGGAGGCTGCCAGGAACCTACATTGTGGTGCTGATGGAGGAGACCCAG AGGCTACAGATTGAACAAACTGCCCACCGCCTGCAGACCCGGGCTGCCCGCCGGGGCTATGTCATCAAGG TTCTACATATCTTTTATGACCTCTTCCCTGGCTTCTTGGTGAAGATGAGCAGTGACCTGTTGGGCCTGGC CCTGAAGTTGCCCCATGTGGAGTACATTGAGGAAGACTCCTTTGTCTTCGCCCAGAGCATCCCATGGAAC CTGGAGCGAATTATCCCAGCATGGCACCAGACAGAGGAAGACCGCTCCCCTGATGGAAGCAGCCAGGTGG AGGTGTATCTCTTAGATACCAGCATCCAGGGTGCCCATCGGGAGATTGAGGGCAGGGTCACCATCACCGA CTTCAACAGCGTGCCGGAGGAGGATGGGACACGCTTCCACAGACAGGCGAGCAAGTGTGACAGCCACGGC ACCCACCTGGCAGGTGTGGTCAGCGGCCGGGATGCTGGTGTGGCCAAGGGCACCAGCCTGCACAGCCTGC GTGTGCTCAACTGTCAAGGGAAGGGCACAGTCAGCGGCACCCTCATAGGCCTGGAGTTTATTCGGAAGAG TCAGCTAATCCAGCCCTCGGGGCCACTCGTGGTTCTGCTGCCCCTGGCCGGTGGGTATAGCCGCATCCTC AACGCTGCCTGCCGGCACCTGGCGAGGACTGGGGTGGTGCTGGTTGCAGCAGCTGGGAACTTCCGGGACG ACGCCTGCCTCTACTCCCCAGCTTCTGCTCCAGAGGTCATCACAGTCGGGGCCACGAATGCCCAGGACCA GCCAGTTACCTTGGGGACTTTGGGGACTAATTTTGGACGCTGTGTGGATCTCTTTGCCCCCGGGAAGGAC ATCATCGGAGCGTCCAGTGACTGCAGCACATGCTTCATGTCACAGAGTGGGACCTCACAGGCTGCTGCCC ACGTGGCCGGCATTGTGGCTCGGATGCTGAGCCGGGAGCCCACACTTACCCTGGCCGAGCTGCGGCAGAG GCTGATCCACTTCTCTACCAAAGACGTCATCAACATGGCCTGGTTCCCTGAGGACCAGCAGGTGCTGACC CCCAACCTGGTGGCCACACTGCCCCCCAGCACCCATGAGACAGGCGGGCAGCTGCTCTGTAGGACGGTGT GGTCGGCACACTCGGGGCCCACTCGAACAGCTACAGCTACAGCCCGCTGTGCCCCAGAAGAGGAGCTGCT GAGCTGCTCCAGCTTCTCCAGGAGCGGGAGGCGTCGTGGTGATTGGATTGAGGCCATAGGAGGCCAGCAG GTCTGCAAGGCCCTCAATGCATTTGGGGGTGAGGGTGTCTATGCCGTCGCGAGATGCTGCCTGGTTCCCC GTGCCAACTGCAGCATCCACAACACCCCTGCAGCCAGAGCTGGCCTGGAGACCCATGTCCACTGCCACCA GAAGGACCATGTTCTCACAGGCTGCAGCTTCCATTGGGAAGTGGAAGACCTTAGTGTCCGGAGGCAGCCT GCGCTGAGGTCCAGACGTCAGCCTGGCCAGTGCGTTGGCCACCAGGCGGCCAGTGTCTATGCTTCCTGCT GCCATGCCCCAGGGCTGGAATGCAAAATCAAGGAGCATGGGATCTCAGGTCCTTCAGAGCAGGTCACTGT GGCCTGCGAAGCAGGATGGACCCTGACTGGATGCAATGTGCTCCCTGGGGCATCCCTCACTCTGGGAGCC TACAGCGTGGACAACCTGTGTGTGGCAAGAGTCCATGACACTGCCAGAGCAGACAGGACCAGTGGAGAAG CCACAGTAGCTGCTGCCATCTGCTGCCGGAGCCGGCCTTCAGCAAAGGCCTCCTGGGTTCAGTGACAGCC TCAGGCAGGGATGGTGCTTGAGGCTGGGTGCAGAGATATGCCTGCATGGCTCTCTTGTAGCCAAAGGTGG GGAGATTCTGCGTGGGAGAACTTGGTGTCTCACCCTGGGTACCCATTCCTGGTGTATGGAAGCACCTCCT TCACGGTCAGGGGGCCTGTGCTTGGCTTTCTGCCATCAGACATTAAGCTGTAGCTGGCTCTGGCCAGCTG CTCCAGTGTACCAGAACCTGAGGATGCTCGCTGCAAGGCCTCAGTTCTCAGGCCTTAGGGTGTATTTGTC TTTCAGGAAGATCATAATGGACAGAGATCCTTGGAGGTTCAAAGACCAAGTACCAGACTGGAAAATTGAG TCTGAAAGCCACAAGGACAGTCAACTCACAGCCAGCTCACATTGCAGACACCATTTTGGGCTCCCTGATT AAATGCAGATCAGTTCTGCACACCTCCAGGGGTGGATCCAGCTGTAAGGCCATACCTATATCTTCCAGAT GTCCTCATCTGCTGCAGGGCTTTGGCCCTGCTCAGGATAATGTGCTATGAGCCCTCATCTGACTCTCAGT TTGTACTGGAGAACCATACAGGACTTACCGCACCTTACCCCATCCACTACCATGTGCACTGACTGGCCTC ATTTTATGAAGGAAGAGACAGGACCAGAGAGGCGATGTCACACAGCCAGTGATGTCAGGACATAAATTCA GAGTGGCTGGCCCTGAATAATGCCAGGCTGGGCAGCGAGAGGACAGGCTATGGCTTGCTCCTGGACCTAT ACTCCCTTAGCCCCAGTCCCACAGATCAGGTGGAGACTGGAGTGACAGAGGGCGACTGTACCAAGGCCAC ACCAGCTGACCAGCACACCTCTATCCTTTTGAGCTCTTCTGTCTTTTTATAGTAAGCTTCCTCCACCTGT GTTGCTTTTGTAACTTGATATTTATGCAGGGTTTTGTAGTTTTTATTATGTAGTGACTTTTCAGAATAAA AGCAGCTGATGTGACTGACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4502199 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4502199 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002970 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source