EMAGE mouse gene expression spatial database

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EMAGE:31104

Gabrg2 gamma-aminobutyric acid (GABA) A receptor, subunit gamma 2 ( MGI:95623)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002924_01	euxassay_002924_02	euxassay_002924_03	euxassay_002924_04	euxassay_002924_05

euxassay_002924_06	euxassay_002924_07	euxassay_002924_08	euxassay_002924_09	euxassay_002924_10

euxassay_002924_11	euxassay_002924_12	euxassay_002924_13	euxassay_002924_14	euxassay_002924_15

euxassay_002924_16	euxassay_002924_17	euxassay_002924_18	euxassay_002924_19	euxassay_002924_20

euxassay_002924_21	euxassay_002924_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex marginal layer

strong

regional

strong expression: see section 08 09 10 13 14 15 16

cervico-thoracic ganglion

weak

regional

weak expression: see section 10 16

spinal cord

strong

homogeneous

strong expression: see section 09 10 11 12 13 14 15 16 17

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 moderate expression: see section 06 weak expression: see section 17 18

thoracic ganglion

weak

regional

weak expression: see section 13 14

telencephalon

strong

regional

strong expression: see section 01 02 03 04 05

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 13 16 17 18 weak expression: see section 07

facial vii ganglion

strong

regional

strong expression: see section 03 04 19 20 21

cornea stroma

strong

regional

strong expression: see section 01 02 22

midbrain

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

vagus x ganglion

moderate

regional

moderate expression: see section 07

diencephalon

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 16 17 18 19 20 21

telencephalon marginal layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

cervical ganglion

weak

regional

weak expression: see section 08 16

telencephalon mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

hindbrain

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4277
Entity Detected:	Gabrg2, gamma-aminobutyric acid (GABA) A receptor, subunit gamma 2 ( MGI:95623)
Sequence:	sense strand is shown >T4277 GTATCTGCAAGCGTTTGTACTGATCTTTATTTCTGCCCCCTGAATATTAATTTTTAAGCTGGTAGCAATA CATCTCTGAGTGACGGGACCTACTAGAGGCAGGTGGGGGGAGCCGGCATCAAATCATCAGCATAATAATA ATACAAAGGGGAGGGATTCTTCTGCAACCCAGAGGCGAGAGGCGAGAGAAAAAAAAAAAGCGATGAGTTC GCCAAATACATGGAGCATTGGAAGCTCAGTCTACTCTCCTGTATTTTCACAGAAAATGACGCTGTGGATT CTGCTCCTGCTATCGCTCTACCCAGGCTTCACAAGCCAAAAGTCAGATGATGACTATGAAGATTACGCTT CTAATAAAACATGGGTGTTGACTCCAAAAGTTCCAGAGGGTGATGTCACTGTCATCTTAAACAACCTGCT GGAAGGGTATGACAACAAACTTCGACCTGACATCGGAGTGAAACCAACATTAATTCATACAGATATGTAT GTGAACAGCATTGGTCCAGTGAATGCTATCAATATGGAATATACAATTGATATTTTTTTTGCCCAAACCT GGTATGACAGACGTTTGAAATTTAACAGTACCATTAAAGTTCTCCGGTTGAATAGCAATATGGTGGGGAA AATCTGGATTCCAGACACTTTCTTCAGGAACTCCAAAAAGGCTGATGCTCACTGGATCACCACTCCCAAC AGGATGCTGAGAATTTGGAATGATGGTCGAGTTCTCTACACCTTAAGGCTAACAATTGATGCTGAGTGCC AGTTGCAATTACACAACTTCCCAATGGATGAACACTCCTGCCCCCTGGAGTTCTCCAGTTATGGATATCC TCGTGAAGAAATTGTTTATCAATGGAAGCGCAGTTCTGTTGAAGTGGGTGACACAAGATCATGGAGGCTG TATCAATTTTCCTTTGTTGGATTGAGGAATACAACTGAAGTAGTGAAGACAACTTCTGGTGACTATGTGG TGATGTCTGTGTACTTCGATCTGAGCAGAAGAATGGGCTACTTCACCATCCAGACTTACATTCCCTGCAC ACTCATCGTGGTCCTGTCCTGGGTGTCCTTCTGGATCAATAAGGATGCTGTTCCTGCCAGAACATCTTTA GGAATCACTACTGTCCTGACCATGACAACTTTAAGCACCATAGCCAGAAAATCTCTGCCCAAGGTCTCCT ATGTCACAGCAATGGATCTCTTTGTATCTGTTTGCTTCATCTTTGTGTTTTCTGCTTTGGTGGAGTATGG CACCCTGCATTATTTTGTCAGCAACCGGAAGCCAAGCAAGGATAAAGACAAAAAGAAGAAAAACCCTCTT CTTCGGATGTTTTCCTTCAAGGCCCCTACCATTGATATTCGTCCCAGATCAGCAACCATTCAAATGAACA ATGCCACACACCTTCAAGAGAGGGATGAAGAATATGGCTATGAGTGTTTGGATGGCAAGGACTGTGCCAG TTTCTTCTGCTGTTTTGAAGATTGCCGAACAGGAGCCTGGAGACATGGGAGGATACATATTCGCATTGCC AAAATGGACTCCTATGCTCGGATCTTCTTCCCTACCGCCTTTTGCTTGTTCAATCTTGTTTACTGGGTCT CCTATCTTTATCTGTAAAGAGGTATGGGTTTCATTGATAGGGGTGTTATTCGCTGAATCTTATGGAGACA AAATGCACTTTCTAAGTCCAACGATATAATCCCCTATGTGGTCACCGAATGTGTTTCTCTGTCTCAACGT AGTAATACATATAATGTCATACTATCCATGCCCAATCCGTGGTTATTGTAATTTGAATTTCAGCGTGCAC TGTCCTTCAAACCTGCAAGGCAAAGTAAAGTTAGAACAAGAGCTGTTACACTGAGCAAGATACCTTTGAG CAACAGCAATGAAAACAGTGGAAGCTGGGAGGGTTTAAAGTGGCATTATCAGTCTTTGACTCATATATAG TTTAGTACAAAAGGTATAAAGCTGTGTACTATGTTCACTTGGGATCAAGTTGTGGTAAACTGGAAAAAAT TAGAATACCTCCCGTATCTGTGAAGAATCACAATAGAAAAGCCTAGATTAGAATTCTATTATACCTTTAT TCCAAGGTAGAAAGCAAATTTCCTTCATATCATATGTACAGTGATGTAAATATTTCAGTAATGTAGAATG CTTCAGGTTTAATGCATGCATCTCTTTAGAGCCAAAATAAATGAACTCATGTTATCATCAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4504205 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4504205 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002924 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source