EMAGE mouse gene expression spatial database

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EMAGE:31106

Dazap2 DAZ associated protein 2 ( MGI:1344344)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002922_01	euxassay_002922_02	euxassay_002922_03	euxassay_002922_04	euxassay_002922_05

euxassay_002922_06	euxassay_002922_07	euxassay_002922_08	euxassay_002922_09	euxassay_002922_10

euxassay_002922_11	euxassay_002922_12	euxassay_002922_13	euxassay_002922_14	euxassay_002922_15

euxassay_002922_16	euxassay_002922_17	euxassay_002922_18	euxassay_002922_19	euxassay_002922_20

euxassay_002922_21	euxassay_002922_22	euxassay_002922_23	euxassay_002922_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

moderate

regional

moderate expression: see section 09 10 11 12

neural retina

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 24

submandibular gland primordium

strong

regional

strong expression: see section 08 09 10 19 20 21 moderate expression: see section 11

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 04 05 24 moderate expression: see section 03

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15 16

vibrissa

moderate

regional

moderate expression: see section 06 07 08 09 22 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 16 19 20 21 weak expression: see section 17 18

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 19 20 21 22 23 24 weak expression: see section 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4294
Entity Detected:	Dazap2, DAZ associated protein 2 ( MGI:1344344)
Sequence:	sense strand is shown >T4294 GAGAAAAATAACCGTCCGCGACGCCGAGTGGGAGCGGACTCAGAGCCACCATGAACAGCAAAGGTCAATA TCCAACACAGCCTACCTACCCTGTGCAACCTCCTGGGAATCCAGTATATCCTCAGACCTTACATCTTCCT CAGGCTCCACCCTATACTGATGCTCCACCTGCATATTCAGAGCTCTATCGTCCAAGCTTTGTTCATCCAG GGGCTGCCACAGTCCCCACCATGTCAGCTGCATTTCCTGGCGCCTCATTGTATCTGCCTATGGCCCAGTC TGTGGCTGTTGGGCCTCTAGGCTCCACAATCCCCATGGCTTATTATCCAGTTGGTCCCATCTATCCACCT GGCTCAGCAGTGTTGGTGGAAGGAGGGTATGATGCAGGTGCCAGATTTGGAGCTGGTGCCACTGCTGGCA ACATTCCTCCTCCACCTCCTGGATGCCCTCCCAACGCTGCTCAGCTCGCAGTCATGCAGGGAGCCAACGT CCTTGTAACTCAGCGCAAGGGAAACTTCTTCATGGGTGGCTCAGATGGTGGCTACACCATTTGGTGAGGA GCCATGGCCACTCCTGTGCCGGGGAAGACATCACATACCTTCAGCACTTCTCACAATGTAACTGCTTTAG TCATATTAACCTGAAGTTGCAGTTTAGACACATGTTTTTGGGGTGTCTTTCTGGTGTCCAAACTTTTGAG GCACTTTTCAAATTTAATAAGGAACCATGTAAGGGTAGCAGCCCCTCCCTAAAGCATTCTGAGGTAAGGA GGTATCCATTCTTCAAATGAATGTGAGTGAAGCAGCCCAAGGCTCTTCCTTAATTCCTCTGGAGTGATGC TGTGCCATGCTGGTCTTTGCTTCTAGTAATAAAACATCAAATTAGGTTTGGAGGGAACTTTGATCTTCCT AAGAATTAAAGTTGCCAAATTATTCTGATTGGTCTTTAATCTCCTTAAGTTTTTGATTTATCTTACTTGG TAAATGAAATGCATTAGCTGTCTGCCTTTTCCTTCCCATCCTCTGCCCAGCCTGTCCACTGTCCCTGCTC CTCCACCCCCTCCCATCACTATCCATAGAATCAGTGGTGCAGGACCGAAAGCCAGACTCAGCCCCTTGTC TCCCTGCACCTGTCCCACTTGACATTTTTTAACTCATCTTTCATAAGGATCCTCTGAAGCCCCCTGTGTG ACCTGAGCACAGACTCCATTTCTTCAGGTTTTGTATATCTCCAGGCAAGGTGAAGGGTACCCTTCGGACC CGCCTCATAAACCATCTCTGCATCACCATGAACCCAGACTAGCTGTGGTGCCAGAAACCTGAACTTAAAC AACAGTTCTCAGTGCAAACTGTCCTAGGAAAATTTAAAGATGACAGGCAGGGGAGAAGATGCATATCAAA AGCTTGGCTTTAAAGTCTAAGGGAGTTTTAGATCAAAAACTAGCTATCACTTCCTGAGTGTCTCATGTAG CCACTCAGGCTCCCAGGAGTTTCCTGAGGTAGGTTAGTAGGGTCACGTGAATGCCTCACTATGACATGAC TTGAGTCCAGTGAGATCTCATTAGGATTAATATTTCAGGGATCCTTAATATTTTGATTTGTTTTCCGAGA TTGGATTTTATTTTATCTGCTATTTCAGTTCATCTAAATCTTGTGTTCTGTACATGTGATGTTTGACTGT ACCATCGACTGTTATGGAAGTTCAGCGTTGTATGTCTCTCTACACTGTGGTGCACTTAACTTGTGGATTT TTTATACTAAAAATGTAGAATAAAGACTATTTTGAAGATTTGAATAAAGTGATGAAGTTGCATTACACCT CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4506124 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4506124 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002922 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source