EMAGE mouse gene expression spatial database

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EMAGE:31109

Camk2a calcium/calmodulin-dependent protein kinase II alpha ( MGI:88256)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002921_01	euxassay_002921_02	euxassay_002921_03	euxassay_002921_04	euxassay_002921_05

euxassay_002921_06	euxassay_002921_07	euxassay_002921_08	euxassay_002921_09	euxassay_002921_10

euxassay_002921_11	euxassay_002921_12	euxassay_002921_13	euxassay_002921_14	euxassay_002921_15

euxassay_002921_16	euxassay_002921_17	euxassay_002921_18	euxassay_002921_19	euxassay_002921_20

euxassay_002921_21	euxassay_002921_22	euxassay_002921_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 18 19 weak expression: see section 17

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 16 17 18 19 20 21 22 23

thymus primordium

moderate

homogeneous

moderate expression: see section 12 13 14 15

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 16 17 18 19 20 21 22 23

telencephalon mantle layer

strong

regional

strong expression: see section 03 04 05 12 13 14 15 16 17 moderate expression: see section 01 02 06 07 08 09 10 11 18 19 20 21 22 23

olfactory cortex ventricular layer

strong

regional

strong expression: see section 10 11 16 17

heart ventricle

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17

head mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 09 22 23

olfactory cortex marginal layer

strong

regional

strong expression: see section 15 16 17 moderate expression: see section 10 11 12 18

tongue

moderate

regional

moderate expression: see section 11 12 13 14 15 16

thalamus mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 16 17 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4291
Entity Detected:	Camk2a, calcium/calmodulin-dependent protein kinase II alpha ( MGI:88256)
Sequence:	sense strand is shown >T4291 GTCAAGCCGGTTCTCCGTTTGCACTCAGGAGCACGGGCAGGCGAGTGGCCCCTAGTTCTGGGGGCAGCGC TTCAGCATCCCAGCCCTAGTTCCCAGCCTAAAGCCTCGCCTGCCTGCCCAGTGCCAGGATGGCTACCATC ACCTGCACCCGATTCACAGAAGAGTACCAGCTCTTTGAGGAACTGGGAAAGGGAGCCTTCTCTGTGGTGC GCAGGTGTGTGAAGGTGCTGGCTGGTCAGGAGTATGCTGCCAAGATTATCAACACCAAGAAGCTTTCAGC CAGAGATCACCAGAAGCTGGAGCGTGAGGCCCGCATCTGCCGCTTGTTGAAGCACCCCAATATCGTCCGA CTCCATGACAGCATCTCCGAGGAGGGGCACCACTACCTTATCTTCGATCTGGTTACTGGTGGGGAACTGT TTGAAGACATTGTGGCCCGGGAGTATTACAGTGAAGCTGATGCCAGCCACTGTATCCAGCAGATCTTGGA GGCTGTGCTACACTGTCACCAGATGGGGGTGGTGCATCGTGACCTGAAGCCTGAGAATCTGTTGCTGGCC TCGAAGCTCAAGGGCGCTGCTGTGAAGCTGGCAGACTTTGGCCTGGCCATAGAGGTGGAGGGGGAGCAGC AGGCATGGTTTGGGTTCGCAGGGACACCTGGATACCTCTCCCCAGAAGTGCTGAGGAAGGACCCGTACGG GAAGCCCGTGGACCTGTGGGCCTGTGGCGTCATCCTGTATATCTTGCTGGTTGGGTATCCCCCGTTCTGG GATGAAGACCAGCATCGCCTGTACCAGCAGATCAAAGCTGGTGCCTATGATTTCCCATCACCAGAATGGG ACACCGTCACCCCAGAAGCCAAGGATCTGATCAATAAGATGCTGACCATCAACCCGTCCAAACGCATCAC GGCCGCTGAGGCTCTCAAGCACCCATGGATCTCGCACCGCTCCACCGTGGCCTCCTGCATGCACAGACAG GAGACCGTGGACTGCCTGAAGAAGTTCAATGCCAGGAGGAAACTGAAGGGAGCCATCCTCACCACTATGC TGGCCACCAGGAACTTCTCCGGAGGGAAGAGCGGAGGAAACAAGAAGAACGATGGTGTGAAGGAATCTTC TGAGAGCACCAACACCACCATTGAGGACGAAGACACCAAAGTGCGCAAACAGGAAATTATCAAAGTGACA GAGCAGCTGATCGAAGCCATAAGCAATGGAGACTTTGAGTCCTACACGAAGATGTGCGACCCTGGAATGA CAGCCTTTGAACCAGAGGCCCTGGGGAACCTGGTGGAGGGCCTGGACTTTCATCGATTCTATTTTGAAAA CCTGTGGTCCCGGAACAGCAAGCCCGTGCACACCACCATCCTGAACCCTCACATCCACCTGATGGGTGAC GAGTCAGCCTGCATCGCCTATATCCGCATCACTCAGTACCTGGATGCAGGCGGCATACCCCGCACGGCCC AGTCAGAGGAGACCCGCGTCTGGCACCGCAGGGACGGCAAATGGCAGATCGTCCACTTCCACAGATCTGG GGCGCCCTCCGTCCTGCCGCATTGAAGGACCAGGCCAGGGTCCCTGCGTCCTTGCTTCGCAGAGATCCGC TCTTTGTCCGTGGAATGTGGCTGCTGGTTCTCCTTTGGATTTTGCTGGAATTCTCCCTGTCAGATCACCC TACCATTGCCACCTATGTACTCGCGTCACGAAAACCTGCTTGTTCACAGAAGTCGCCACGACATCACAGT GAACAGCCAGCTCTCCCCAGCTCCGTTGCCCAAGCTCTTCCTGCCAGTGGGGACCTTCTTCCGGCTTAAG TACCCAGGGTGCTGGCCCCAGGAACCCCCACCCCCTACCCACTGTTGTTGGCCTAGCCTAGCTTTAGCTA TAGATGGGGCCTCAGCTGTGCAATTGGCAGGAAGTGAGGAAGAGGCAGGCAAGCTGTGTTGAGGGCACCT CTCATCGATTCCTTCTTTCCTGGGGTTCCCCGGGGAAGCTCACACGAGGCCCTCAGTCTCCAAGCCAACC CCTTATGAGGGAGAGTGAGAGAGGAGCCAACGCCAGTGAGCCAGGAACTGCTGCTCTCATCTGCTCTCCT CTGTGTTGGCCTTGCCTTTGACCAGACCATCCGCTACGAGGGGTGGGCTCTACCGCCCAGGTGCCCCACT CACTCTGCCTCAGTCCTCCTGTGAAGTTTGCCTCCAGTGTTGACCCACCCACCCTGCCCTTCAACGTCCT TGGAGAATTCCAGCTTCATCTGTCTGAGAGGAGATTGGAAGGTGTTTCAGGGGCAAAGCAAGCAACATTT AGGTATCACTTCTACTTGGACGCATGCCTTTTTACAGCCAAACTTCCGTGTATTTCGTAAATGGATTTTG CGTTAACGGACATCTATGTGATAACTAGACCTCTCAAGTTTACTGTAAAGAAGAGTCGGATGGGTTGGGA AGTGGGTGGGAAGAGGAGTGAGAGGAAGTTTTAACCCCATTCCAGAGGGTCTTTTTTTTGGGGGGTGTCC CTTCTGGGGGAGGGTGCTTTCTGAGGTGGCCTCACCCCCCAGGGAGCATGGTTTCCTTCCATTATGGTCC CCAAAGTCAGCTGACAAGATTTCTTCCAGAGCCAGCATGACTAACACACAGTGAGTCAGGTCAGGGGAGG CTATCAGGAAGGGGTTATCTAGACTTGGCATCTCTGGAACTGGAGACCTCACTACCCTACTTCCCAGGAA ATCTTTCCCGTATCCTTGTGCTATCTCTCCTCAGCCCAGCCCCCACTCACCACCTTCTAGGCACATTATT CTCATCACCTCTCTCAACTTGGCCAAGCAAGAGTGCCAGAAACTTATTTTCCTACTTCATGCTGAACTTG GCCTGGTGTGCGCTCTATTGTGCTTGCCTGCCTCCCTGGCCTTCCTCGACTCCCCTGCCCCGACATACAC ACACACACACACACACACACACACACGCACACGCACACGCGCGCACACACACACATGCACACGCACATGC ACACACTCACACCACTTCCTTCCACCACTTCCTTCCTCCCCCCTCTCCCTCCCTTTCTCTTTCTCTTCTC TCCCTCTTCCTGGGTTTGGCTCTTGTATGGAATGCTGTATCTCATTCACGGGGATCTCCTGTCTGCACTG TTTTCTTTGCATGACTTTATATGCAGTAAGTATGTTGAAAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4506005 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4506005 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002921 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source