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EMAGE:31113

Fam83f family with sequence similarity 83, member F ( MGI:2146227)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31113 EMAGE:31113 EMAGE:31113 EMAGE:31113 EMAGE:31113
euxassay_002927_01 euxassay_002927_02 euxassay_002927_03 euxassay_002927_04 euxassay_002927_05
EMAGE:31113 EMAGE:31113 EMAGE:31113 EMAGE:31113 EMAGE:31113
euxassay_002927_06 euxassay_002927_07 euxassay_002927_08 euxassay_002927_09 euxassay_002927_10
EMAGE:31113 EMAGE:31113 EMAGE:31113 EMAGE:31113 EMAGE:31113
euxassay_002927_11 euxassay_002927_12 euxassay_002927_13 euxassay_002927_14 euxassay_002927_15
EMAGE:31113 EMAGE:31113 EMAGE:31113 EMAGE:31113 EMAGE:31113
euxassay_002927_16 euxassay_002927_17 euxassay_002927_18 euxassay_002927_19 euxassay_002927_20
EMAGE:31113 EMAGE:31113 EMAGE:31113 EMAGE:31113
euxassay_002927_21 euxassay_002927_22 euxassay_002927_23 euxassay_002927_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
stomach
moderate moderate
regionalmoderate expression: see section 04 05 06 07 09
rectum
weak weak
regionalweak expression: see section 15 16
urethra of male
weak weak
regionalweak expression: see section 15 16
midgut
moderate moderate
regionalmoderate expression: see section 11 12 13 17 18 weak expression: see section 14 15 16 19 20 21
bladder
weak weak
regionalweak expression: see section 15 16
esophagus
moderate moderate
regionalmoderate expression: see section 14 15
pancreas
moderate moderate
regionalmoderate expression: see section 11 12 13 14 17 18
lower jaw molar
moderate moderate
regionalmoderate expression: see section 07 08 20 21
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 09 10 11 18 19 20 21 22 weak expression: see section 08
pharyngo-tympanic tube
moderate moderate
regionalmoderate expression: see section 01 02 03 04 24
upper jaw molar
moderate moderate
regionalmoderate expression: see section 07 08 20 21
thymus primordium
moderate moderate
homogeneousmoderate expression: see section 13 17 weak expression: see section 14 15 16
vibrissa
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 21 22 23 24
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 13 17
hindgut
moderate moderate
regionalmoderate expression: see section 13 17 18 weak expression: see section 14 15 16
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 12 13 17 18 weak expression: see section 16
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 12 13 17 18 weak expression: see section 16
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T320
Entity Detected:Fam83f, family with sequence similarity 83, member F ( MGI:2146227)
Sequence:sense strand is shown

>T320
GGGACAGCAGGGTCCGAGCCTGGGGCCCCGGGACAGGGCTGGGGCGCCATGGCCGAGTCCCAACTGAGCT
GCCTGGACGAGGCGCACGTGAACGAGAGGGTGACCGAGGCACATGCAGCTTTCTACTACTGCGAGAGGCG
GCGGGCCGCGCTGGAGGCGCTGCTGGGTGGAGGCGAGCAGGCCTACCGCGAACTGGTCAAGAAGGAGCGG
CTGCGGGACTTCCTCTCCAGCCAGGAGCGCCAGGCCCTCTGCGCCGCCTGGAGCCCCTACGAGGAGGCAG
TCTCCAGCACCCGTGCCAAGGCTAAGGCCAAGGCCAAGGTCCCGGCCCAGCCCACTGAGTCCCTGGCCTA
CTGGCCAGACCGCTCGGACACCGAAGTGCCCCCACTGGACCTGGGGTGGACAGACACCAACTTCTACCGC
GGTGTGAGCCGAGTCACGCTCTTCACCCACCCGCCCAAAGAAGAGAAGGCACCGCACCTGAAGCAGGTGG
TCAGGCAGATG
Notes:The probe template was PCR amplified from IMAGE:3153977 using vector specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3153977 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002927 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS