EMAGE mouse gene expression spatial database

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EMAGE:31114

Cck cholecystokinin ( MGI:88297)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000508_01	euxassay_000508_02	euxassay_000508_03	euxassay_000508_04	euxassay_000508_05

euxassay_000508_06	euxassay_000508_07	euxassay_000508_08	euxassay_000508_09	euxassay_000508_10

euxassay_000508_11	euxassay_000508_12	euxassay_000508_13	euxassay_000508_14	euxassay_000508_15

euxassay_000508_16	euxassay_000508_17	euxassay_000508_18	euxassay_000508_19	euxassay_000508_20

euxassay_000508_21	euxassay_000508_22	euxassay_000508_23	euxassay_000508_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon

strong

regional

strong expression: see section 01 02 17 18 19 20 21 23 24

hypothalamus

strong

regional

strong expression: see section 12

midbrain

strong

spotted

strong expression: see section 09 10

diencephalon

strong

regional

strong expression: see section 02 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2142
Entity Detected:	Cck, cholecystokinin ( MGI:88297)
Sequence:	sense strand is shown >T2142 TGCTNCCTTNTCCGCTGGAACTCGCCAAGCCAGCTGATTCCCCATCCAAAGCCATGAAGAGCGGCGTATG TCTGTGCGTGGTGATGGCAGTCCTAGCTGCTGGCGCCCTGGCGCAGCCGGTAGTCCCTGCAGAAGCTACG GACCCCGTGGAGCAGCGGGCGCAAGAGGCGCCCCGAAGGCAGCTGCGGGCTGTGCTCCGGACGGACGGCG AGCCCCGAGCGCGCCTGGGCGCACTGCTAGCGCGATACATCCAGCAGGTCCGCAAAGCTCCTTCTGGCCG CATGTCCGTTCTTAAGAACCTGCAGAGCCTGGACCCCAGCCATAGAATAAGTGACCGGGACTACATGGGC TGGATGGATTTTGGCCGGCGCAGTGCCGAGGACTACGAATACCCATCGTAGTGGGCCAGCGTCTTGGCCC TGCTTGGAGGAGGTGGAATGAGGAAACAACCACACATACGACCCCTCGCCTCTAATGTCTGACGTTTTGA GTATCTATTTATTAAGTCCCCAATGTGAAATCT
Notes:	The probe template was PCR amplified from IMAGE:874454 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:874454 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000508 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source