EMAGE mouse gene expression spatial database

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EMAGE:31122

Slc27a2 ( MGI:1347099)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_011400_01	euxassay_011400_02	euxassay_011400_03	euxassay_011400_04	euxassay_011400_05

euxassay_011400_06	euxassay_011400_07	euxassay_011400_08	euxassay_011400_09	euxassay_011400_10

euxassay_011400_11	euxassay_011400_12	euxassay_011400_13	euxassay_011400_14	euxassay_011400_15

euxassay_011400_16	euxassay_011400_17	euxassay_011400_18	euxassay_011400_19	euxassay_011400_20

euxassay_011400_21	euxassay_011400_22	euxassay_011400_23	euxassay_011400_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vomeronasal organ

strong

regional

strong expression: see section 13 16

renal cortex

strong

regional

strong expression: see section 10 22 23 moderate expression: see section 21 weak expression: see section 11

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 15 16 17 18 19 20

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

pituitary gland

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

cochlea

strong

regional

strong expression: see section 05 22 moderate expression: see section 09 20 21 weak expression: see section 06 07 08 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30099
Entity Detected:	Slc27a2, ( MGI:1347099)
Sequence:	sense strand is shown >T30099 CATCGTGGTTGGGGCTACTTTAGCTTTGCGGAGCAAATTCTCAGCCAGCCAGTTTTGGGACGACTGCAGG AAATACAACGTCACGGTCATTCAGTACATTGGTGAACTGCTTCGGTACCTGTGCAACACACCGCAGAAAC CAAATGACCGGGACCACAAAGTGAAAAAAGCCCTGGGAAATGGCTTACGAGGAGATGTGTGGAGAGAGTT CATCAAGAGATTTGGGGACATCCACGTGTATGAGTTCTACGCATCCACTGAAGGCAACATTGGATTTGTG AACTATCCAAGGAAAATCGGTGCTGTCGGGAGAGCAAACTACCTACAAAGAAAAGTTGCAAGGTATGAGC TGATCAAGTATGACGTGGAGAAGGACGAGCCGGTCCGTGACGCAAATGGATATTGCATCAAAGTCCCCAA AGGTGAGGTTGGACTCTTGGTTTGCAAAATCACACAGCTCACACCATTTATTGGCTATGCTGGAGGAAAG ACCCAGACAGAGAAGAAAAAACTCAGAGATGTCTTTAAGAAAGGCGACATCTACTTCAACAGCGGAGACC TCCTGATGATCGACCGTGAGAACTTCGTCTACTTTCACGACAGGGTTGGAGATACTTTCCGGTGGAAAGG AGAGAACGTAGCTACCACAGAAGTCGCTGACATCGTGGGACTGGTAGATTTTGTTGAAGAAGTGAATGTG TATGGCGTGCCTGTGCCAGGTCATGAGGGTCGAATTGGGATGGCCTCCCTCAAGATCAAAGAAAACTACG AGTTCAATGGAAAGAAACTCTTTCAACACATCGCGGAGTACCTGCCCAGTTACGCGAGGCCTCGGTTCCT GAGGATACAAGATACCATTGAGATCACTGGGACTTTTAAACACCGCAAAGTGACCCTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4159081), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 16855. Forward Primer - name:016855_F_IRAV20-23_N17_Slc27a2, sequence:CATCGTGGTTGGGGCTAC; Reverse Primer - name:016855_R_SP6_IRAV20-23_N17_Slc27a2, sequence:TCAGGGTCACTTTGCGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_011400 same experiment
	EMAGE:31275 same embryo
	EMAGE:29357 same embryo
	EMAGE:31210 same embryo
	EMAGE:29348 same embryo
	EMAGE:31248 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source