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EMAGE:31124

Pde9a phosphodiesterase 9A ( MGI:1277179)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31124 EMAGE:31124 EMAGE:31124 EMAGE:31124 EMAGE:31124
euxassay_005191_01 euxassay_005191_02 euxassay_005191_03 euxassay_005191_04 euxassay_005191_05
EMAGE:31124 EMAGE:31124 EMAGE:31124 EMAGE:31124 EMAGE:31124
euxassay_005191_06 euxassay_005191_07 euxassay_005191_08 euxassay_005191_09 euxassay_005191_10
EMAGE:31124 EMAGE:31124 EMAGE:31124 EMAGE:31124 EMAGE:31124
euxassay_005191_11 euxassay_005191_12 euxassay_005191_13 euxassay_005191_14 euxassay_005191_15
EMAGE:31124 EMAGE:31124 EMAGE:31124 EMAGE:31124 EMAGE:31124
euxassay_005191_16 euxassay_005191_17 euxassay_005191_18 euxassay_005191_19 euxassay_005191_20
EMAGE:31124 EMAGE:31124 EMAGE:31124 EMAGE:31124
euxassay_005191_21 euxassay_005191_22 euxassay_005191_23 euxassay_005191_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
oral epithelium
weak weak
regionalweak expression: see section 09 10 11 12 13 14 15 16 17 18 19
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 05 06 07 17 18 19 20 21
metanephros
moderate moderate
regionalmoderate expression: see section 07 08 09 11 17 18 19 21 weak expression: see section 10 20
cervico-thoracic ganglion
weak weak
regionalweak expression: see section 16
spinal cord
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15
rectum
moderate moderate
regionalmoderate expression: see section 15 16
trigeminal v nerve
moderate moderate
regionalmoderate expression: see section 10
bladder
moderate moderate
regionalmoderate expression: see section 14 15 16 17
lower jaw molar
weak weak
regionalweak expression: see section 08 09 20
upper jaw molar
weak weak
regionalweak expression: see section 08 21
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 04 05 06 07 08 18 19 20 21 22
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 05 10 11 12 13 14 15 16 17 18 19 20
cervical ganglion
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 17
naris
weak weak
regionalweak expression: see section 13 14 15 16 17 18
tongue epithelium
weak weak
regionalweak expression: see section 12 13 14 15 16 17 18
mesenchyme
moderate moderate
regionalmoderate expression: see section 13 weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24
stomach
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 weak expression: see section 03 04 05 06 07
brain
strong strong
regionalstrong expression: see section 02 03 04 05 moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 07 18
retina
strong strong
regionalstrong expression: see section 05 moderate expression: see section 02 03 04 24
midgut
strong strong
regionalstrong expression: see section 17 moderate expression: see section 13 14 15 16 18 weak expression: see section 19 20 21 22
tongue mesenchyme
weak weak
regionalweak expression: see section 12 13 14 15 16
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 07 08 10 11 12 13 15 16
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 08 09 17 18 19 20 weak expression: see section 10 11
tail
strong strong
regionalstrong expression: see section 17 18 19 moderate expression: see section 16 20 weak expression: see section 14 15
limb
moderate moderate
regionalmoderate expression: see section 09 10 weak expression: see section 01 02 03 04 05 06 07 08
lower jaw incisor
weak weak
regionalweak expression: see section 13 17 18
upper jaw incisor
weak weak
regionalweak expression: see section 13 17
lung
moderate moderate
regionalmoderate expression: see section 05 07 08 09 15 16 17 18 19 21 22 weak expression: see section 02 03 04 06 10 11 13 14 20 23
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9625
Entity Detected:Pde9a, phosphodiesterase 9A ( MGI:1277179)
Sequence:sense strand is shown

>T9625
AAGGTGAAAAAGTCCAAGTGCAGCTGCGGGCGCGATGGGGGCCGGCTCCTCAAGCTACCGGCCCAAGGCC
ATCTACCTAGACATCGATGGACGCATCCAGAAGGTGGTTTTCAGCAAGTACTGCAACTCCAGTGACATCA
TGGACCTGTTCTGTATCGCCACCGGCCTGCCTCGGAACACCACCATCTCCCTTTTAACCACAGACGACGC
CATGGTCTCCATCGATCCCACCATGCCTGCGAATTCAGAGCGCACTCCCTACAAGGTGAGACCTGTGGCT
GTGAAGCAAGTGTCTGAACGAGAAGAACTTATCCAGGGCGTGCTGGCCCAGGTGGCGGAACAATTTTCCA
GAGCGTTTAAGATCAACGAGCTGAAAGCCGAAGTTGCAAATCACCTGGCCGTGCTGGAGAAACGGGTGGA
ATTGGAAGGACTTAAAGTGGTGGAGATCGAAAAATGCAAGAGTGACATTAAAAAGATGCGGGAGGAGTTG
GCAGCTAGGAACAGCAGGACCAACTGTCCATGTAAATACAGTTTTTTGGATAACAAGAAGTTGACACCTC
GACGTGATGTCCCCACTTACCCCAAGTACCTGCTCTCCCCAGAGACCATCGAAGCCCTACGGAAGCCCAC
CTTTGATGTCTGGCTTTGGGAACCCAACGAGATGCTGAGCTGTTTAGAACATATGTACCACGACCTTGGT
CTGGTCAGGGACTTCAGCATCAACCCAATCACGCTCCGCAGGTGGCTGCTCTGTGTGCATGACAACTACA
GGAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGTGTGACACAGATGATGTACAGTATGGTCTGGCT
CTGTGGCCTCCAGGAGAAGTTTTCCCAGATGGACATCTTGGTCCTGATGACTGCAGCCATCTGCCATGAC
CTGGACCACCCAGGGTACAACAATACATACCAGATCAATGCCCGCACGGAACTCGCTGTGCGCTACAACG
ACATCTCACCGCTGGAGAACCACCACTGCGCCATTGCCTTCCAGATCCTGGCAAGACCTGAGTGCAACAT
CTTCGCCAGTGTGCCACCCGAGGGCTTCAGGCAGATCAGGCAGGGGATGATCACATTGATCCTGGCTACC
GACATGGCAAGGCATGCTGAAATTATGGATTCTTTCAAAGAAAAGATGGAGAATTTTGACTACAGCAACG
AGGAGCACCTGACCCTGCTGAAGATGATTCTCATAAAATGCTGTGATATCTCCAATGAAGTCCGTCCCAT
GGAGGTGGCAGAACCGTGGGTGGACTGTTTACTGGAAGAATATTTTATGCAGAGTGACCGTGAGAAGTCC
GAAGGCCTTCCTGTGGCCCCATTCATGGACCGAGACAAAGTGACCAAAGCAACAGCCCAAATTGGGTTCA
TCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCGTTGTTGAGGAGACCATGCT
GCGGCCGCTCTGGGAGTCCCGAGAACACTACGAGGAGCTGAAGCAGCTGGACGATGCCATGAAGGAGTTG
CAGAAGAAGACAGAGAGCTTAACATCTGGGGCCCCGGAAAACACCACAGAGAAGAACAGAGATGCAAAAG
ACAGTGAAGGCCATTCTCCTCCAAACTGATGGACATCTCAATGTGTGCAACTGTACCAGTTAGAGCAGAT
GAATTGTGGCCTGTGAGTGGACAGAGCCAAGCGAGGCTTCCCAGGATCTTCCACACAAGGATGGTCACGC
CCAGACGACCCCTGATGACCTGCTACAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATATAAAAAC
AGGAATTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6533665 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6533665 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_005191 same experiment
 EMAGE:29173 same embryo
 EMAGE:29596 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS