EMAGE mouse gene expression spatial database

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EMAGE:31137

Syt13 synaptotagmin XIII ( MGI:1933945)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002933_01	euxassay_002933_02	euxassay_002933_03	euxassay_002933_04	euxassay_002933_05

euxassay_002933_06	euxassay_002933_07	euxassay_002933_08	euxassay_002933_09	euxassay_002933_10

euxassay_002933_11	euxassay_002933_12	euxassay_002933_13	euxassay_002933_14	euxassay_002933_15

euxassay_002933_16	euxassay_002933_17	euxassay_002933_18	euxassay_002933_19	euxassay_002933_20

euxassay_002933_21	euxassay_002933_22	euxassay_002933_23	euxassay_002933_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 09 18 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 09 18 19

thalamus mantle layer

strong

homogeneous

strong expression: see section 09 10 11 12 13 16 17 18 19

hypothalamus mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 12 13 16 17 18 19 20

facial vii ganglion

strong

regional

strong expression: see section 04 05 moderate expression: see section 21 22

cornea stroma

strong

regional

strong expression: see section 02 03 04 05 24

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 06 07 08 09 10 11 12 13 19 20 21 22 23 24

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 18 19 20 23 moderate expression: see section 21 22

upper lip

strong

regional

strong expression: see section 13 14 15 16 moderate expression: see section 12 18 19 weak expression: see section 17

telencephalon mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 19 20 22 23 24 weak expression: see section 17 18 21

midbrain mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

glans of male genital tubercle

strong

regional

strong expression: see section 14 15 16 17 18 19

lower lip

strong

regional

strong expression: see section 13 14 15 16 moderate expression: see section 12 weak expression: see section 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4254
Entity Detected:	Syt13, synaptotagmin XIII ( MGI:1933945)
Sequence:	sense strand is shown >T4254 GAGCGGCTGGAGCCAGGCGGGAGCGCGAGGTGCAGGAGGCCGGCGGGAGGCGGGCGAGCCGCGGGAAGCA GGCACCATGGTGCTGTCGGTGCCTGTGATCGCGCTGGGCGCCACGCTGGGCACTGCCACCAGCATCCTTG CGCTGTGCGGGGTCACTTGCCTGTGCCGACACATGCACCCCAAGAAGGGGTTGCTGCCGCGGGACCGTGA GCCCGACCCGGAGAAGGCAAGGCCCGGTGTGCTTCAGGCGGCCCAACAGTTCAACATTAAAAAGTCCACG GAGCCTGTCCAGCCCCGACCTCTCCTTAAGTTCCCGGACATCTATGGTCCCCGGCCAGCCGTGACGGCCC CAGAAGTCATCAACTACGCAGACTACACCTTGGAGACCACAGAGGAGTCTGCTGCACCTGCCAGCCCCCA GGCCCAGAGTGACAGTCGCCTCAAGAGGCAGGTCACAGAGGAGCTGTCCATCCGTCCTCAGAACGGTGTA GTTGAGGATGTCTGTGTCATGGAGACCTGGAACCCAGAGAAGGCTGCCAGCTGGAACCAGGCCCCCAAAC TCCACTTCCGCCTGGACTATGACCAGAAGAAGGCTGAGTTATTTGTGACAAGCCTGGAAGCTGTCACCAG TGACCATGAAGGAGGCTGTGACTGTTACATCCAAGGCAGCGTGGCTGTCAAGACCGGCTCTGTGGAGGCA CAGACAGCTCTGAAGAAGCGGCAGCTGCACACAACCTGGGAGGAGGGCCTGGCCCTGCCCCTGGGTGAGG AAGAGCTCCCCACAGCTACCCTGACACTCACCCTGCGCACCTGTGACCGCTTCTCCCGCCATAGTGTGAT TGGGGAACTCCGCCTGGGCCTGGATGGAGCATCTGTACCCCTGGGGGCAGCACAGTGGGGTGAGCTGAAG ACCACAGCAAAGGAGCCATCTGCAGGGGCCGGGGAGGTCCTTCTGTCAATCAGCTACCTCCCAGCTGCCA ATCGCCTCCTGGTGGTGCTGATTAAAGCTAAGAACCTCCACTCCAACCAATCCAAGGAGCTGCTGGGCAA GGATGTGTCTGTCAAGGTGACCTTGAAACACCAGGCTCAGAAGCTGAAGAAGAAACAGACAAAACGGGCC AAGCACAAGATCAACCCCGTGTGGAACGAGATGATCATGTTTGAGCTACCGGATGACTTGCTGCGGGCAT CCAGTGTGGAGCTGGAGGTGCTGGGCCAGGGCGAGGAGGGGCCGAGCTGCGAGCTGGGCCACTGCAGCCT AGGCCTGCATGCCTCGGGCTCTGAGCGCAGCCACTGGGAGGAGATGCTAAAAAACCCACGCCGGCAGATC GCCATGTGGCACCAACTGCACCTGTAGTCGGCCACCTGCCACCGGCCACTGACCGTCTTTCTTGGACACA GTCATCTTCCAGGCTCAGCTGTCAGTCATCCCTCGTGCTCTGTCCACATGCCTATACCCAAAGACAGATG CAGACGTGTTGGCTGAGGCCAAGACCTCATTCCTCTCTTACTGTATTCCCAATACTAAAAAGTGAGCGGT GGCGCCAGGACAGTGAGTGGGACTGGCACTCAGAAGACTGTATCATGCTCATCTGTGTTATGGAAAAAGA TATGTGCCAGGTACCCTCAGTCGGCAGCCCCTGCTCACATTGAGCTGAAGCAGTGGTTCTTAACCTGTGT ATCACAACCCTCTATCTCCAAAAAATATTTACATTATGATTCATAACAGTAGCAAAATTACAGTTACAAA GTAGCAATGAGAACAATTCTGTGGTTGGAGATCACCAAAACACAAGAAACTTTCTTATAGGGTCACAGCA CTAGGAAGGTTGAGAATCACTGGCTGGCCAGCAGGTCTCTGGGCCACTGGGGGGTCATGCCACACTGATG TTCCGGTGGAATACACAAGCCTCTGTCCTTAGCTAGCACAGGGGTTCTGGAGCAGAGCAGGAGGGGCTGC GCTTGATAAGATCCCTTCTCCCCTATCCAGGCAGCCTTTCCTCTTGATCTCCGGGGGCCCAGCACCAGGA CAGTGTGAAGGCAGGAACTCTCTGCTTACTTTATTTCTTCTTTTTTATATTTCTGCTGTTCATATTCCAC TCTCGAGCTACATTGTGTGTTTTGACTGTTTTATTTAAGGGATTGGAGTGGGTTGTGTTTGTTTTTTTTT TATCACACGTAGAGTGCCCGTTCCCTGGCAGGCCTAGGTTGCCACTCCTCTGGAGTGTCTGCGGCATTCT GAGGACAGCTGTCATGTCACCCCCCCCCACCCCCAAGCTGACTCCATGTGACAGACCTGTTCTTCTCAGT ATGCCTGTACTTCAGGACGAAGTAGCTCTGAGCTTGGCTTGTTCCATCTGGCTATGTAAATGAGGAGGTA GATGAACTTTAAAGAGAAAACGTGGTCTCTTGATAACATGCCTCCCTCCAGTTTGGGGGACATGTCCTTT AACTTGGAAATAACAAAAAGCTAGGTTTGATTGCCCCTGTTGATATTTCCAATAGATGCCCCACTAGGAA TCAAATAAGTAGCTAGCTGCAGGGGTAGATACTTGTGAGCCGGAATTCGAGTTGTCATTACTTCCTTGCT CCACAGCGCCTCAGTGTGGCCACCTATAGCACAGCACCAATGTCATGGGAGCTTGCCAGTCCAGCTTCAG GAGGTAAGCAAGAGGCATCTTAGCGGGCATCAATTGTTACTGGTCAGTTGGTGGCACTTCCTGGCTCCTC CGTTGGCTGTGCTTCTTCTCCAAAACCTCCTCTGTAAATTGCTCAGAGATGTTAGACTGGGAACCAAAAA CTTTCAGTCACCTTTTACATCGGCTTAATCTGTTTCCAGCCATTCCTGTAAGTCACCTCCGAATCAGCTT GGGAAGGAACCTCTCTATGTCTTTGCCAGGGAGTGATTTCTGGAAAGAGGTGGAAATAAAGTGAGAGGCC ATTCCAGGCTGGGCTACTGCTGGGAGGTTAGGAGTTCACCCCAGTCCTCTGTGAATCCTCTCCCCAGGAC AACTAGGCATCTATTTGTTGAGTGTTTGTCCACCAAGACCGTGCATGTGTCTGTCCATTCTGATTAGAGA AGGCTCTGGGGGCGAGTGAGTAGCTTATCACCTTAACGGAAACATCAGATAAGAGGAGTCCGGGACTGCT GTTCAGGCGGGCAGCTCTCAGATGTAAAGGGGCCCCGGGGGAAGGGGCAGTGTCTGGCCTGAACTGAGGT GGGAATCTTTGGAGGAGAAAGTTGGTCCTGTGCAGTGTTTCTACCCTTGCTGTTTCCTGTTGTTAGATGC TGCGTGTTTGTGATGCCATTCCACATAAAAGCACAAAAAGTCGCATGAGACATCGTGGTCACATGTCTGG TTACACTTTGGAGCACAAAATCTTACAGTGGTAAATAAATCGTTTTCCAATCGAAAAAAAAAAAAAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4503633 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503633 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002933 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source