EMAGE mouse gene expression spatial database

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EMAGE:31141

Vit vitrin ( MGI:1921449)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002936_01	euxassay_002936_02	euxassay_002936_03	euxassay_002936_04	euxassay_002936_05

euxassay_002936_06	euxassay_002936_07	euxassay_002936_08	euxassay_002936_09	euxassay_002936_10

euxassay_002936_11	euxassay_002936_12	euxassay_002936_13	euxassay_002936_14	euxassay_002936_15

euxassay_002936_16	euxassay_002936_17	euxassay_002936_18	euxassay_002936_19	euxassay_002936_20

euxassay_002936_21	euxassay_002936_22	euxassay_002936_23	euxassay_002936_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

strong

regional

strong expression: see section 09 10 15 16 17 moderate expression: see section 11

ventral grey horn

strong

single cell

strong expression: see section 11 12 13 weak expression: see section 14

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14

pons ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 moderate expression: see section 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 18

cochlea

strong

regional

strong expression: see section 01 02 22 23 24

spinal cord ventricular layer

strong

regional

strong expression: see section 11 12 13

dorsal root ganglion

weak

regional

weak expression: see section 08 09 10 16 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 17 18 19 20 21 22

telencephalon ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 11 12

midbrain ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

hypothalamus ventricular layer

strong

regional

strong expression: see section 10 13 moderate expression: see section 11 12

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 10 13 moderate expression: see section 11 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4336
Entity Detected:	Vit, vitrin ( MGI:1921449)
Sequence:	sense strand is shown >T4336 GAACTGGATAGGAATGTTTGCTTGGCTCGCCTGAGAAGAGAAAAGATTCCTCGACCTCCTCTTGCTTCTT CTTTAGAATAATTTGGATGGGATTTGTGATGCAGAAAAGCTTAAAGAAAAAAGGATAACCGTTCTTTGTG GTGGAAAGTTTAGGGGGGAAAAAACCCACTGCTTTCTTCAAACAAGGGTGCCATTCTGATATTTATGGGA ATTGTTGTTCCCACTATGAAGGCATCTGTTATTGAAGTGTTGCTTGTTTTGCTGGTAACTGGAATACATT CAAATAAAGAAACGCCAAAGAAGACTAAAAGGCCCAAACTCACTGTGCCTCAGATCAACTGCGATGTCAA AGCTGGAAAGATCATCAATCCTGAGTTCATGGTGAAATGTCCAGCGGGCTGCCAGGACCCCAAATACCAT GTTTACGGCACTGGCGTCTACGCGTCTTACTCCAGCGTGTGTGGCGCCGCAATTCACAGCGGTGTGCTGG ATAATTCAGGAGGGAAAATACTTGTTCGGAAAGTTGCTGGGCAGTCTGGTTACAAAGGAAGCTACTCGAA TGGGGTTCAGTCTTTATCTTTACCCAGATGGCGAGAGTCCTTCATCGTCGCAGAAAGTAAGCCCCAAAAG GGTGTAGCCTACCCATCGACTCTTACATATTCATCATCGAAAACTGCAGCTGCTAAAGCAGGTGAGACCA CAAAAGCCTATGAGAAGCCATCTATTCCAGGGACAACCATACAGCCTGTCACCCTGACACAGGCTCAAGC TACCCCTGTAGCTGAGGTCACCCACAGATCCACCTCAAAGCCATTTGCAGCATCTGTCACCAACAGCCCC AGACCCCAACCTGTGGGCCACAGGAGTCAGGAGATGGAAGAGGTGGACGGGTGGAAGCCTGGACCAGTCC TTTTAGATTCAGGATTTGTGCCAAAAGAAGAACTCAGCACACAGTCTTCGGAGCCAGTGCCGCAGGGAGA TCCAAACTGCAAAATTGACTTGTCCTTCTTAATTGATGGGAGCACCAGCATCGGCAAGCGCCGCTTCCGG ATCCAGAAGCAGTTCCTAGCGGACGTCGTCCAGGCTCTTGACATCGGCCCTGCCGGTCCCCTCGTGGGTG TTGTTCAGTACGGAGACAATCCTGCTACCCAGTTTAACCTCAAAACTCACATGAATTCTCAAGATCTGAA GACAGCTATAGAGAAAATTACCCAGAGAGGAGGCCTTTCTAATGTAGGCCGTGCCATCTCCTTTGTAACC AAGACCTTCTTTTCCAAAGCCAATGGGAACAGAGGTGGTGCTCCCAATGTGGCTGTGGTCATGGTGGATG GCTGGCCCACAGACAAGGTGGAAGAGGTGTCACGAGTTGCCAGAGAGTCTGGGATCAACGTCTTCTTCAT CACCGTCGAAGGGGCCGCCGAAAGGGACATACAGCATGTGGTGGAGCCCGGTTTTGCCAGCAAGGCAGTG TGCAGAACAAACGGGTTCTACTCCTTCAATGTGCAGAGCTGGCTCAGCCTTCACAAAACAGTGCAGCCCC TGGTGAAGCGGGTCTGCGACACGGACCGCCTAGCCTGCAGCAAGACCTGTTTAAACTCTGCGGACATAGG CTTTGTCATCGATGGGTCTAGCAGCATGGGGACAAGCAACTTCCGCACGGTCCTGCAGTTCGTGGCCAAC CTCAGCAAGGAGTTTGAGATTTCAGACACCGACACACGCGTCGGGGCCGTGCAGTACACCTACGAGCAGC GGCTCGAATTTGGGTTCGACAAGTATAACAGCAAAGCCGACATCCTCAGTGCCATCAGGAGGGTGGGGTA CTGGAGCGGAGGCACCAGCACAGGGGCTGCCATCCAGTATGCCCTCGAACAGCTCTTCAAGAAGTCCAAA CCGAACAAGAGGAAGGTAATGATCATTATCACTGACGGCAGGTCTTACGATGACGTGCGGATCCCAGCCA TGGCTGCCTACCAGAAGGGGGTGATCACGTATGCAATAGGCATTGCGTGGGCTGCTCAGGACGAGCTGGA GGTAATGGCCACTCACCCCGCCAAGGACCATTCCTTCTTTGTGGATGATTTTGACAACCTCTACAAAATT GCCCCCAGGATCATCCAGAATATTTGTACAGAGTTCAACTCACAGCCTCGAAACTGAGCTGGGACCAGAC AGAGGATGGCAAACACTGTCTTACGGATCCTTGGGACCATCCCTCGCCTTGTGCCTATGGGTGGGGGTGG AGTGGGGGGTAGGGGGAGTTTCTGACAGAGCTTGGGAAAAGGCGTTTGGTGTTACTATTCTTTGCCATTG GTGGTGTGCATGCTTCAAAACCTACCAGGTAAAAGGCTGGCTAGGAGCTCTCAGGTTGGGTTCAAATACT GCATCGCTTTGTGTGCACTGAAGAATCCACATTTTGACAGTTGTTTTCAAAATAGTTAATCCAGAGGAGA GTCCAGCTCTTAGGACAGGAAAGACCTGAGAGGTTATAGGAAGTTTGTTTACATTTCCCATTAGAACTCT GTAATTGTCCATAAGTATCTTTTTATTGTTTTCTTCCCCTTTTTTACTTTCTTTTTCTTCTTTTTTGTCA TGAGAATGTGGGAGTTGCTGAAATAAATGTTTCAAAGAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4527027 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4527027 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002936 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source