EMAGE mouse gene expression spatial database

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EMAGE:31148

Gria4 ( MGI:95811)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006297_01	euxassay_006297_02	euxassay_006297_03	euxassay_006297_04	euxassay_006297_05

euxassay_006297_06	euxassay_006297_07	euxassay_006297_08	euxassay_006297_09	euxassay_006297_10

euxassay_006297_11	euxassay_006297_12	euxassay_006297_13	euxassay_006297_14	euxassay_006297_15

euxassay_006297_16	euxassay_006297_17	euxassay_006297_18	euxassay_006297_19	euxassay_006297_20

euxassay_006297_21	euxassay_006297_22	euxassay_006297_23	euxassay_006297_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

strong

regional

strong expression: see section 01 02 03 04 22 23 24

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 17 18

stomach

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10

metanephros

strong

regional

strong expression: see section 05 06 07 08 09 14 15 16 17 18

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 10 15 16

spinal cord

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15

male reproductive system

strong

regional

strong expression: see section 10 11 13

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

trigeminal v nerve

strong

regional

strong expression: see section 09 16 17

thoracic ganglion

strong

regional

strong expression: see section 10 11 12

midgut loop

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19 20 21 22

bladder

strong

regional

strong expression: see section 10 11 12 13

dorsal root ganglion

weak

regional

weak expression: see section 07 08 09 13 14 15 16 17 18

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 19 20 21

not examined

regional

not examined expression: see section 01 02 03 04 22 23 24

lower jaw molar

strong

regional

strong expression: see section 07 19

vagus x ganglion

strong

regional

strong expression: see section 07 08 09 17 18

upper jaw molar

strong

regional

strong expression: see section 07 17 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21

vibrissa

moderate

regional

moderate expression: see section 06 07 19 20

adrenal cortex

strong

regional

strong expression: see section 06 07 08 14 15 16 17

cervical ganglion

strong

regional

strong expression: see section 08 09 16

adrenal medulla

strong

regional

strong expression: see section 07 weak expression: see section 15

not examined

regional

not examined expression: see section 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8816
Entity Detected:	Gria4, ( MGI:95811)
Sequence:	sense strand is shown >T8816 GGGGACTGAGACCATGGGGAGGTGCTAACTTCCTCTCCAACCCCCCAGCCCGGGATCCGGGCTGGCATAC CCGGCCCCCCGGGGTAGTTTCCTGCCAAGCTCAACCAAGAATCCAAAGGCGACTTGGAGGGCATAAAAAG AAAAACACTGGTTCTGAAGAGGGGCCCCCACCTCCAGAGCCGGTTCCTCGGCAAACGCGCGGAAGGTAGG AGGAAGGGGATCAGATACTGGAGACTGTACGCCAAAACCCCAGCCTGAAGACTCGCTCCAGAGCCTCCCT GAAGACCTGGGCGATCTGCGCCATCATCTTCAATGCCTCTCTGAAAATCATTTAGCAAGAGTGAGAGAAA GAGAGGAGAGCGCGCACCAGGGAGAGGAGCAAAGAAGATGAGGATTATTTGCAGGCAGATTGTCTTGTTG TTTTCTGGATTTTGGGGACTCGCCATGGGAGCCTTTCCGAGCAGCGTTCAAATAGGTGGTCTCTTTATCC GAAACACAGACCAGGAATACACTGCTTTTCGGCTAGCTATCTTTCTTCATAACACCAGCCCCAATGCATC TGAAGCCCCTTTCAATTTGGTACCTCATGTGGACAACATTGAGACTGCCAACAGTTTTGCTGTGACAAAT GCATTCTGTTCCCAGTATTCTAGAGGGGTGTTTGCCATTTTTGGACTCTATGACAAGAGGTCAGTGCATA CCTTGACCTCCTTCTGCAGTGCTCTGCACATCTCTCTCATCACACCAAGCTTCCCCACTGAAGGAGAGAG CCAGTTCGTGCTTCAGCTAAGACCTTCATTGAGAGGTGCACTCCTGAGCCTCCTGGATCACTATGAATGG AATTGTTTTGTCTTCCTGTATGATACAGACAGGGGGTATTCAATACTTCAAGCTATAATGGAAAAAGCAG GACAGAATGGATGGCATGTCAGTGCGATATGTGTGGAAAATTTTAACGATGTCAGCTACAGGCAACTACT AGAAGAGCTTGACAGAAGACAAGAGAAGAAATTTGTAATAGATTGTGAGATAGAAAGGCTTCAAAACATA TTAGAACAAATTGTGAGTGTTGGGAAGCACGTCAAAGGCTACCATTATATCATCGCAAATTTGGGTTTCA AAGATATTTCTCTTGAGAGATTTATACATGGAGGAGCAAATGTCACTGGATTCCAGTTAGTAGATTTTAA TACGCCCATGGTGACGAAACTAATGGATCGCTGGAAGAAACTAGATCAACGAGAATATCCAGGATCTGAA ACACCTCCAAAGTACACTTCTGCTCTCACTTACGATGGTGTCTTGGTAATGGCTGAAACTTTCCGAAGTC TCAGAAGACAGAAAATTGATATTTCAAGGAGAGGAAATGCCGGGGATTGTCTGGCAAACCCTGCTGCTCC CTGGGGCCAGGGAATTGACATGGAGAGAACACTGAAGCAGGTTCGAATTCAAGGACTGACTGGGAATGTT CAATTTGACCACTATGGACGTAGAGTTAATTACACAATGGATGTGTTTGAATTAAAAAGCACAGGACCTC GAAAGGTTGGCTATTGGAACGATATGGATAAATTAGTCTTGATTCAAGATGCGCCTACTCTTGGCAATGA CACAGCAGCTATCGAGAACAGAACAGTGGTTGTAACCACAATTATGGAATCCCCCTACGTTATGTACAAG AAAAATCATGAAATGTTTGAAGGAAATGACAAGTATGAAGGCTACTGTGTAGACTTGGCATCTGAAATTG CGAAACATATCGGTATCAAATATAAAATTGCCATTGTCCCTGATGGAAAATATGGAGCAAGGGATGCGGA CACCAAAATTTGGAATGGGATGGTAGGAGAGCTTGTGTATGGGAAAGCAGAGATTGCCATTGCACCTCTG ACAATCACGTTGGTGCGAGAGGAGGTCATCGACTTTTCTAAGCCTTTTATGAGTTTAGGCATCTCTATCA TGATCAAAAAACCTCAGAAATCTAAACCAGGAGTGTTTTCCTTCTTGGACCCTCTGGCCTATGAGATCTG GATGTGCATAGTGTTTGCATACATTGGTGTCAGCGTGGTCTTGTTCCTAGTCAGTAGGTTTAGCCCATAT GAGTGGCACACAGAAGAGCCTGAGGATGGAAAAGAAGGACCCAGTGACCAACCTCCCAATGAGTTTGGCA TCTTTAACAGCCTCTGGTTTTCCCTGGGTGCCTTTATGCAACAAGGATGTGACATTTCACCCAGATCCCT GTCCGGTCGGATTGTTGGAGGCGTATGGTGGTTCTTCACTCTCATCATTATCTCATCCTACACTGCTAAT CTGGCTGCATTCCTGACAGTGGAGAGAATGGTCTCCCCCATAGAAAGTGCAGAAGACCTGGCCAAACAAA CAGAAATTGCCTATGGAACACTTGATTCGGGATCAACAAAAGAATTCTTCAGAAGATCAAAAATAGCAGT ATATGAAAAGATGTGGACCTACATGCGATCGGCAGAGCCATCTGTGTTCACTAGAACTACAGCTGAGGGC GTGGCCCGTGTCCGCAAGTCCAAGGGCAAATTTGCCTTCCTCCTGGAGTCCACGATGAATGAATACATTG AGCAGCGAAAGCCCTGTGACACGATGAAAGTGGGAGGAAACCTGGATTCCAAAGGCTATGGTGTAGCGAC GCCCAAGGGTTCCTCATTAAGAACTCCTGTAAACCTTGCCGTTTTGAAACTCAGTGAGGCAGGCGTCTTA GACAAGCTGAAAAACAAATGGTGGTACGATAAAGGTGAATGTGGACCCAAGGACTCGGGAAGCAAGGACA AGACGAGTGCCTTGAGCCTGAGCAACGTAGCAGGCGTCTTCTACATTCTGGTTGGCGGCTTGGGCTTGGC AATGCTGGTGGCTTTGATAGAGTTCTGTTACAAGTCCAGGGCAGAGGCGAAGAGAATGAAGCTGACCTTT TCCGAAGCCATAAGAAACAAAGCCAGGTTATCCATCACTGGGAGTGTGGGAGAAAACGGCCGTGTGCTGA CCCCCGACTGCCCCAAGGCCGTACACACAGGAACTGCGATTAGACAGAGCTCGGGATTGGCTGTCATTGC ATCGGACCTACCATAAAAACCAAAAAATTAATTGAGTGCCTTAATCAAACTGTGTTGGTGACTGATGGAA ACGCAGCCCCGAGGGACAGGCCAAGCGTGGGTCTTGGCTAAACCCATCCTTTGACTGAGCAGGAAGGACA TCCTGAGTGCCGGACAGAACCAGTGCATGAGTTCCGCACGGAAATGCAGACTCGGATTTTTTTATCAGGA AATCTCACAGTTTAGTTTTTTGGGGGGAGTGGGTGGGGTACCTTAGGTGTATTAACAGCAACAGATCTCA CTTGAGCGCACTTAAAACTAATCAGACTGGCCAGTTAGCGCATTAAACTGTGAAGTTCTTTCTCAGAAAG GTCGTGGTCACCACAGCCAGGGATCAACAGTTAATAGAGGTCAAAGAACCTGCTACCTGTGTCTCCAGAA CACCCATATAGTCCATGGAATAATCTTCCGCTGAGACAAGCCGTCACTAACATGTGATAGGAAAATAATA AATAGATAAAGCCTGTGAACAAACAATGAGATTTAAAGAAAGTATTTACAGTTACTTTGGAAATAAAACA ATACTGAAACATGCTTGCTTTTTAACTGAAGTTAATCCAGTAGAGGACAACGCAATTGTTTTTTCTAACC ATCTTAGGGAACAATACATTGCAATAATTGATAATAGTGCCATCACTGTAATAAACTTTAGAGACTTTTT TTAATGTAAAAGTTGTTGGTCACCTTGTTTCCTGTAACCTTCACTCTGTCACACAAGTTGGCTCATAGGT TGTGTTTGTCTATCAGAAATAAGAAAAACACAAGTGAAGAAAATGTTGGCATGAAGTCATCCATCTGCAA TGAAAAACCTAAAAGACTACGGGTCACTCATGTTATCAATATAATTTATAATCCTGTTCAGTGTACAAAA TTGTGGGTTTTGTACTCACCCAAAAGACTAAAACACCAGTTTTTCTTACAGTATCTATCTACAGAGCTTA TTCTCCCCTATTATTTGGGAAACTCTGAGACTCCATATTGCAGAAGTCAAGGAATAGGCCATATAAGAAA ATGTAGCTTGTTTTTATTATTTCTGCATATTTATTTCTAGATCTTGGGCTCATTTGTTAACAGAATAAGT TGTCAAAGGTAAAGTCCTTGAGTCTGGGAATGAGCCATCGTTCCAAAACCAACACACCCTGTGTGGAAAT TTTACTTGACTCTGTTTTGCTGCATAGAATTCAGTGTCTCTTGGCCATTCCCCCTCATTCCTATACTAAA TTCTTTGAAGACACTGGTAACAGTTTGTGGTAGACTACAGTTGAAAAAACTCAATCCTTATTTCTACCAA GTAATTGTGAGTAAAATTATACCATGATCATTTTTAAGAAAAGAAAACCCTTATGGTTCAAAAGGTCTGG TTCATTTGAAATGATGAATGAGTTCAATGATACAGTTTTTAAAAAACCAAAACAAACAAACAAACAAAAA CAAATCACAGATTGAGGGATCTTTGCAGAAAGCACAAATTAGGATGATGCCCAAAGTGTGGCCTTGAAGA ATGAGTTGAGTTTTCACAGGAGAAGGTGCTGGTAAGATCCTAGGCGTGAGCAGCACACATAGCTGCTTCT TTTATCAGTGTTTCATGTACAAGGCTTGTGCAACCAGGTGGCCTGGTGAGGGCACAGGGTGTGACACCAT GGAAGCTCTGTCAGAAATATGGAGAATTGGTTTCCTTCCCTCAATACTTACAATCCCATTCAATAACTGT GTGTCACTACAATGTAATCCTTATAATACATGTATGCACTGTCCTTTGGGAAAGGTTACTTCTCTGTTGT TGAGCAATGATTCATTTCACAACCTAGGCCACTCCCTGGTATCTTCCTCTTCCTCTCTCACTCCTACCCA AGCTCTCAGCCAGCAAATGGACCTCCACAGCACACGGAACACGGCATCTACTGTGTCTTTGATCTGTAGA TCTTTTTTGTCTTTACCTGACTGTCACACATCCTTCAGCCCTGAAATTGAATTTTATGTGTACTGAGAAG ATTTCCATGATTACCCACCACCCTGCCCATGACACTGAGTTACCTGCCATTCTTAATGTTTCTTACCCAT CATGGCACTCATCGTACTGCATTGTAATTGCCTGTGTACTTGTCTATAAACTACTAGACTGTCAGCTCCT TGAGGGCAGGGACTGTGTCTATTTTGTTCACAGTTGTATCCCCAGCACCCAGCACAGTGCCTGGCATATT GTAGGTGCTTAATAAATATTTGTTGAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6409918 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6409918 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006297 same experiment
	EMAGE:30034 same embryo
	EMAGE:30007 same embryo
	EMAGE:30026 same embryo
	EMAGE:31126 same embryo
	EMAGE:31599 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source