EMAGE mouse gene expression spatial database

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EMAGE:31151

Rbbp7 retinoblastoma binding protein 7 ( MGI:1194910)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000288_01	euxassay_000288_02	euxassay_000288_03	euxassay_000288_04	euxassay_000288_05

euxassay_000288_06	euxassay_000288_07	euxassay_000288_08	euxassay_000288_09	euxassay_000288_10

euxassay_000288_11	euxassay_000288_12	euxassay_000288_13	euxassay_000288_14	euxassay_000288_15

euxassay_000288_16	euxassay_000288_17	euxassay_000288_18	euxassay_000288_19	euxassay_000288_20

euxassay_000288_21	euxassay_000288_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 moderate expression: see section 06 07 08

metencephalon rest of alar plate ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 14 15 16 17 18 19

cranium

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3585
Entity Detected:	Rbbp7, retinoblastoma binding protein 7 ( MGI:1194910)
Sequence:	sense strand is shown >T3585 GAAATTGTGGATGCTAAAGCAATCTTTACTGGCCACTCAGCTGTTGTAGAGGATGTGGCCTGGCATCTGC TGCATGAGTCCTTGTTTGGATCTGTTGCTGATGATCAGAAACTTATGATATGGGACACCAGATCCAATAC CACTTCTAAGCCGAGCCATTTGGTGGATGCACACACCGCTGAGGTCAACTGCCTCTCATTCAATCCCTAC AGCGAGTTCATTCTGGCAACTGGCTCTGCAGATAAGACTGTAGCTTTATGGGACCTGCGTAATCTGAAAC TAAAACTCCACACCTTTGAATCGCATAAGGATGAAATTTTCCAGGTCCACTGGTCTCCACATAATGAAAC TATTCTGGCCTCAAGTGGTACTGATCGCCGCCTGAATGTGTGGGATTTAAGTAAAATTGGAGAAGAACAA TCAGCAGAAGATGCAGAAGATGGGCCTCCAGAGCTCCTGTTTATTCATGGAGGGCACACTGCCAAGATTT CTGACTTCAGCTGGAATCCCAATGAACCTTGGGTCATTTGCTCTGTGTCTGAA
Notes:	The probe template was PCR amplified from IMAGE:3167347 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3167347 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000288 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source