EMAGE mouse gene expression spatial database

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EMAGE:31157

Nsf ( MGI:104560)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004886_01	euxassay_004886_02	euxassay_004886_03	euxassay_004886_04	euxassay_004886_05

euxassay_004886_06	euxassay_004886_07	euxassay_004886_08	euxassay_004886_09	euxassay_004886_10

euxassay_004886_11	euxassay_004886_12	euxassay_004886_13	euxassay_004886_14	euxassay_004886_15

euxassay_004886_16	euxassay_004886_17	euxassay_004886_18	euxassay_004886_19	euxassay_004886_20

euxassay_004886_21	euxassay_004886_22	euxassay_004886_23	euxassay_004886_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 13 14 15 16 17

vagus x ganglion

moderate

regional

moderate expression: see section 08 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vibrissa

weak

regional

weak expression: see section 06 07 19 20 21

spinal cord

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 weak expression: see section 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6898
Entity Detected:	Nsf, ( MGI:104560)
Sequence:	sense strand is shown >T6898 CGGACGCGTGGGCGCGCGCGGCGGAGGCGGGCTCAGCAGAGTGCGGAGCTGCAACCTGGGAGCCGGACGT GTCCGCGAAGATGGCGGGCCGGACTATGCAAGCTGCGAGATGCCCTACTGATGAATTATCTTTAAGCAAC TGTGCGGTTGTGAACGAAAAAGATTTCCAGTCTGGCCAGCATGTGATGGTGAGGACGTCCCCCAATCACA AGTACATATTTACGCTGAGGACCCATCCGTCAGTGGTTCCAGGATGCATCGCGTTCAGCTTGCCTCAGCG AAAATGGGCTGGACTTTCTATTGGACAGGACATAGAAGTTGCCTTGTATTCGTTTGACAAAGCCAAGCAG TGCATCGGCACAATGACCATCGAGATCGACTTCCTGCAGAAGAAGAACATCGACTCCAACCCTTACGACA CCGACAAGATGGCCGCCGAGTTCATCCAGCAGTTCAACAACCAGGCCTTCTCAGTGGGGCAGCAGCTTGT CTTTAGCTTCAATGATAAGCTCTTTGGATTACTGGTGAAGGACATTGAAGCCATGGATCCCAGCATCCTG AAGGGAGAGCCTGCATCAGGCAAAAGGCAGAAGATTGAGGTAGGCCTGGTTGTTGGAAACAGTCAAGTTG CATTTGAAAAAGCAGAAAATTCGTCGCTCAATCTTATCGGCAAAGCTAAAACCAAGGAAAATCGCCAGTC CATCATCAATCCCGACTGGAACTTTGAGAAAATGGGGATCGGCGGTCTGGATAAGGAGTTCTCGGACATT TTCCGAAGAGCATTTGCTTCTCGGGTGTTCCCCCCGGAGATCGTGGAACAGATGGGTTGCAAACATGTTA AAGGCATCCTGTTATACGGACCCCCTGGTTGTGGTAAAACTCTCTTGGCTCGACAGATTGGCAAGATGCT GAATGCGAGAGAGCCCAAGGTGGTCAATGGGCCAGAAATCCTTAACAAGTATGTGGGAGAATCAGAGGCT AACATTCGTAAACTCTTTGCTGATGCCGAAGAGGAGCAAAGGAGGCTTGGTGCTAACAGTGGCTTGCACA TCATCATCTTTGATGAAATCGATGCCATCTGCAAGCAGAGAGGGAGCATGGCCGGCAGCACTGGAGTGCA TGACACCGTGGTCAACCAGCTGCTGTCCAAGATCGATGGCGTTGAGCAGCTGAACAACATCCTCGTTATC GGAATGACCAATAGACCAGATTTGATAGACGAAGCTCTCCTTCGACCCGGAAGACTGGAAGTTAAAATGG AGATAGGCTTGCCAGATGAGAAGGGTCGACTCCAGATCCTTCATATCCACACAGCAAGGATGAGAGGGCA CCAGTTACTGTCTGCAGATGTGGACATCAAGGAGCTGGCTGTGGAGACTAAGAATTTCAGTGGCGCTGAG CTGGAGGGTCTGGTGCGAGCAGCGCAGTCCACAGCCATGAACAGACATATCAAGGCCAGCACCAAAGTAG AAGTAGACATGGAGAAGGCAGAGAGCCTGCAGGTGACAAGAGGGGACTTCCTTGCTTCCCTGGAGAACGA TATCAAACCAGCATTTGGCACCAACCAAGAGGATTATGCAAGTTACATTATGAATGGGATCATCAAGTGG GGTGACCCCGTGACCCGAGTCCTAGATGACGGAGAGCTGCTGGTCCAGCAAACGAAAAACAGTGACCGGA CACCACTCGTTAGTGTCCTCCTAGAAGGGCCTCCTCACAGCGGGAAGACTGCCCTGGCTGCAAAGATTGC CGAGGAATCCAACTTCCCCTTCATCAAGATCTGCTCACCTGATAAGATGATTGGCTTCTCCGAGACAGCC AAGTGTCAGGCCATGAAGAAGATCTTTGATGATGCTTACAAATCTCAGCTCAGCTGTGTGGTCGTGGATG ACATTGAGAGGCTGCTCGATTATGTCCCCATTGGTCCCCGGTTCTCCAACCTGGTATTGCAGGCTCTTCT TGTGTTACTGAAAAAGGCGCCTCCTCAGGGCCGCAAGCTTCTTATCATCGGGACCACCAGCCGCAAAGAT GTCCTGCAGGAAATGGAGATGCTGAACGCCTTCAGCACCACCATCCACGTGCCCAACATTGCCACAGGAG AGCAGCTGCTGGAAGCTCTGGAGCTTTTGGGCAACTTCAAAGATAAGGAACGCACCACAATTGCTCAGCA AGTCAAAGGGAAGAAAGTCTGGATAGGAATCAAGAAGTTACTAATGTTGATTGAGATGTCCCTGCAGATG GATCCTGAGTACCGTGTGAGGAAATTCTTGGCCCTCATGAGAGAAGAAGGCGCTAGTCCCCTGGACTTTG ATTGAAAACGGACTGTTTACAACAGTGACCAAGGGAAGCGACCAAGGTGCAGATGGCCTGGGATCGTCAC TCCTCACACTCAAGACACTGGACTACGTGAAGTGTCCGCCTCTCTCCACACACTCGCTCTGCATGACTAG TGCAGTGAACTCCCTCGTGCTTTTGAGATGTTCTCGTGTTCCCTGGACTGCTGGGCTTCCCTCCCGTGCT AGCTAAGATGGTTCCTGCTTATGTGGGAGGCATGTCACACCCGTGCACGCTGCCCACACCGCGATCCCTA CCACCTGCCCGAAGGTCAACTTCTACATGAGACCAAGAGCAAAGGTAGAGGTTCCAAGGGAGTCCCTTGG CGCTCCCCATGCTAACAGCTGTGTATCCCGCAGATTAGGTCATTGTCTGTTCCTAAAGAACTAGGCTTTG AATATCTAAGATGCTGTTCTCAATCATTTTCAGAGATATTTCCAAGTTGCAATCAGGAGAGCTTTTCTAA TCGGAAGTCAAATCATTCCTGGGGTGGCTGTGATATGCTCTGAGTGTAATTAATTTGCTCTGTGGTAAGA GATATGCTAATCATTGCCACTTGGTAGATGCCGTTAAAACCTTATGAAAGATACATATGGAAATCTGTAC ATCCCTAAACAGAAAAAAAATTAAAATAATTTACAAGCCTCTCAATTCTAATTAGCGTCCGGCTCTAAGT GGGTCAGTTCCTTAGGATGACGTTAGAGGCCTATTAGTGCCATCGTCTTCCTTTTCCTCAGCCTGATGAC TTACAATTCATCCTCCTTGTATCTCAAGTGATCTGTAGTGTCTGCAGCACATAAATAAAACACTACTCTC CAGCAGAACAAGCCACCCCCTAGTCCTCTCCCTCAGCCCTCGAATATCCATTCCAGCGTACTCAACACAC CTCTTCCCGAAGAGGAACTCAGCGAGCCCTTTGGAGGGTGGTTTGCCTAATCAGTGGGTTAGAAGGAATG CAATTAGGTCGTCACGTCATTCGTGGAGATGAGTCTTAGGGCGCATGCCACTCTCCCCGCTGCTGCTTCA AACACAAAGACAGCCATCAGAAGCCGCCCGGGAGGCTTGCTGCCGCAGGGGCACCTGGGTGATACTCTGA CACTGTCCCCACAGTAGAAGGCAAATCATCTCCTAGTCTTGCCGTCTGTATACCTTGTTTGGGGTTTTCA TCCCTGGCTTATTTTGTGGCTTTGAAAGAGTGAATTGTGTTGTGAAACTGTGATTCCCGTGGCTAGTACC GTGGTTCCCTGTCCACCACCGTGGTCCCTGTTGTCTGCAGTTCTGCCTTTCTCCTCCCAACAAAGTCCTA CATGAATTAATTTATGTTTCTTGTTCAACGAAGTTAATGTCTTGTGTTTTTTGTCCAAAATTATATTGAA AAAGATATTATATTGTGTAATGCAAATTGAAGGAATGCAATAAAGAGTAAATATACTTGAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3968733 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3968733 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004886 same experiment
	EMAGE:30956 same embryo
	EMAGE:30118 same embryo
	EMAGE:30448 same embryo
	EMAGE:30133 same embryo
	EMAGE:31019 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source