EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31164

Lancl1 LanC (bacterial lantibiotic synthetase component C)-like 1 ( MGI:1336997)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_012275_01	euxassay_012275_02	euxassay_012275_03	euxassay_012275_04	euxassay_012275_05

euxassay_012275_06	euxassay_012275_07	euxassay_012275_08	euxassay_012275_09	euxassay_012275_10

euxassay_012275_11	euxassay_012275_12	euxassay_012275_13	euxassay_012275_14	euxassay_012275_15

euxassay_012275_16	euxassay_012275_17	euxassay_012275_18	euxassay_012275_19	euxassay_012275_20

euxassay_012275_21	euxassay_012275_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 07 13

facial vii ganglion

weak

regional

weak expression: see section 02 03 18 19

vagus x ganglion

strong

regional

strong expression: see section 06 15

thymus primordium

weak

regional

weak expression: see section 09 10 12

trigeminal v ganglion

weak

regional

weak expression: see section 01 02 03 04 05 06 15 16 17 18 19 20

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 12 13 14 moderate expression: see section 08 09 15 weak expression: see section 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 15 16 17 moderate expression: see section 04

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 moderate expression: see section 16 weak expression: see section 04

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30413
Entity Detected:	Lancl1, LanC (bacterial lantibiotic synthetase component C)-like 1 ( MGI:1336997)
Sequence:	sense strand is shown >T30413 ACAGTGAGAAGCAGGCCGAGGAGTGCATCACACGGCTCATTCACCTAAATAAGATTGATCCCCACGTACC AAATGAAATGCTCTATGGCCGCATAGGCTACATCTTTGCTCTGCTTTTTGTCAATAAGAACTTTGGAGAG GAGAAGATTCCTCAGAGCCATATTCAGCAGATTTGTGAAAACATCTTAACCTCTGGGGAAAACCTATCTA GAAAGAGAAACTTGGCGGCAAAGTCTCCACTGATGTATGAGTGGTACCAGGAATATTACGTGGGGGCTGC TCATGGCTTGGCAGGCATTTATTACTACCTGATGCAGCCCAGCCTTCAGGTGAACCAAGGAAAGTTGCAT AGTTTGGTGAAGCCCAGTGTAGACTTTGTCTGCCGGCTGAAGTTTCCTTCCGGCAATTACCCTCCATGTT TGGATGATACCAGAGACCTGCTTGTCCATTGGTGCCACGGTGCTCCTGGGGTCATCTATATGCTCATCCA AGCATACAAGGTGTTCAAAGAAGAGCGTTACCTGTGTGATGCCCAGCAGTGTGCTGACGTGATCTGGCAG TACGGGCTACTGAAGAAGGGCTACGGGCTGTGCCATGGTGCTGCAGGGAATGCCTACGCTTTCCTGGCAC TCTACAACCTCACACAGGATCTGAAGTATCTGTACAGGGCCTGCAAGTTTGCTGAGTGGTGCTTAGACTA CGGGGAGCACGGATGCAGGACAGCGGACACCCCCTTCTCCCTCTTTGAAGGGATGGCTGGAACAATATAT TTCTTGGCTGACCTGTTAGTCCCCACAAAGGCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6315377), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60182. Forward Primer - name:060182_F_IRAV104_a09_Lancl1, sequence:ACAGTGAGAAGCAGGCCG; Reverse Primer - name:060182_R_SP6_IRAV104_a09_Lancl1, sequence:TGGCCTTTGTGGGGACT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_012275 same experiment
	EMAGE:29836 same embryo
	EMAGE:29837 same embryo
	EMAGE:30152 same embryo
	EMAGE:31471 same embryo
	EMAGE:31470 same embryo
	EMAGE:29833 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source