EMAGE mouse gene expression spatial database

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EMAGE:31168

Tmprss2 transmembrane protease, serine 2 ( MGI:1354381)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_010099_01	euxassay_010099_02	euxassay_010099_03	euxassay_010099_04	euxassay_010099_05

euxassay_010099_06	euxassay_010099_07	euxassay_010099_08	euxassay_010099_09	euxassay_010099_10

euxassay_010099_11	euxassay_010099_12	euxassay_010099_13	euxassay_010099_14	euxassay_010099_15

euxassay_010099_16	euxassay_010099_17	euxassay_010099_18	euxassay_010099_19	euxassay_010099_20

euxassay_010099_21	euxassay_010099_22	euxassay_010099_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

stomach fundus

moderate

regional

moderate expression: see section 02 03 04 05 06 07 weak expression: see section 01 08

kidney pelvis

weak

regional

weak expression: see section 08

hindgut

weak

regional

weak expression: see section 11 12 13 14 15 16 17

kidney calyx

weak

regional

weak expression: see section 07 16 17 18 19

not examined

regional

not examined expression: see section 14 15 16

bladder

moderate

regional

moderate expression: see section 11 12 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T32130
Entity Detected:	Tmprss2, transmembrane protease, serine 2 ( MGI:1354381)
Sequence:	sense strand is shown >T32130 GCATTTGCGGGAATTCTGAGACAGTCTCTCATGTTCTATGGAAGTAGACACCAGGTAGAAAAAGTAATTT CCCATCCAAATTACGACTCTAAGACCAAGAATAACGACATTGCTCTCATGAAGCTGCAGACACCTTTGGC TTTTAATGATCTAGTGAAGCCAGTGTGTCTGCCGAACCCAGGCATGATGCTAGACCTAGACCAGGAATGC TGGATTTCGGGGTGGGGGGCCACCTATGAGAAAGGGAAGACCTCGGACGTGTTGAATGCTGCCATGGTAC CCTTGATCGAGCCCTCCAAATGTAATAGTAAATACATATACAACAACCTAATCACACCAGCCATGATCTG TGCCGGCTTCCTCCAGGGGTCTGTCGACTCTTGCCAGGGAGACAGTGGAGGGCCGCTGGTTACTTTGAAG AATGGGATCTGGTGGCTGATTGGGGACACGAGCTGGGGCTCGGGCTGTGCCAAGGCACTCAGACCTGGAG TATACGGGAACGTGACGGTATTTACAGATTGGATCTACCAGCAAATGAGGGCGAACAGCTAATCCACGTG GCTTTGTCCCAGACTTCCTTTGTCTTCAACAACCTTCTGCAAGAAAACCAAGGGCCTGAATTTTAACTTC CTGTGCACAATGTACCTTTTGAGATGATTCGAAGGGCCTTTCACTTTTATTAAACAGTGACTTGTTTGAC TGTGCTCCCTGGTCCTGTGAGGGCTTCAGTGCCCCACCCCTGGGCCACTTCTGCAGCTCCCACCAGAATG GATGACCAGATTCTGTTGGGTTTGGGCACATAGGGCCAAAGGCAGAGGAGGGTGGCACTCTCATGTTGGA ACTTCTTTTGGGCTCATGCTCAGGCCTTTTTTGGATCACTAAGGACTATGACCTCTGAGTAACCTGATGA CCTGAGAAAGAGTAAGGAGGCCAGGCAGGGCCTTGGGCCCAGGAACAGGTACCTTGAGAGTGAGAGCTAC CCATTGCCTGTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:2648889), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59200. Forward Primer - name:059200_F_IRAV94_e09_Tmprss2, sequence:GCATTTGCGGGAATTCTG; Reverse Primer - name:059200_R_SP6_IRAV94_e09_Tmprss2, sequence:GCCACAGGCAATGGGTA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_010099 same experiment
	EMAGE:29418 same embryo
	EMAGE:29371 same embryo
	EMAGE:29324 same embryo
	EMAGE:31190 same embryo
	EMAGE:29404 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source