EMAGE mouse gene expression spatial database

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EMAGE:31174

Mfap2 microfibrillar-associated protein 2 ( MGI:99559)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001133_01	euxassay_001133_02	euxassay_001133_03	euxassay_001133_04	euxassay_001133_05

euxassay_001133_06	euxassay_001133_07	euxassay_001133_08	euxassay_001133_09	euxassay_001133_10

euxassay_001133_11	euxassay_001133_12	euxassay_001133_13	euxassay_001133_14	euxassay_001133_15

euxassay_001133_16	euxassay_001133_17	euxassay_001133_18	euxassay_001133_19	euxassay_001133_20

euxassay_001133_21	euxassay_001133_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

skeleton

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1055
Entity Detected:	Mfap2, microfibrillar-associated protein 2 ( MGI:99559)
Sequence:	sense strand is shown >T1055 TCNAGNCTGTTGGCCTACTGGACTTTCAGCCGCACTAGAGCACAGCGCAGAGGNGCCAACAGCTGTCCTC AACGCCACCACCGCCTCCTCACTCCATGGCCATGAGAGCCGCCTGCCTCTTCCTGCTGTTCATGCCTGGC CTGCTGGCTCAGGGCCAATATGACCTGGATCCTCTCCCCCCATTCCCGGACCATGTCCAGTACAACCACT ATGGCGACCAGATTGACAACGCAGACTACTATGACTACCAAGAAGTGAGTCCTCGGACCCCTGAAGAGCA GTTCCAGTCCCAGCAGCAAGTTCAACAGGAAGTCATCCCAGCCCCTACCCCAGAACCAGCAGCTGCAGGG GACCTGGAGACTGAGCCTACCGAGCCTGGCCCTCTTGACTGCCGCGAAGAACAGTACCCGTGTACCCGCC TCTACTCCATCCACAAGCCTTGCAAACAGTGTCTCAACGAGGTCTGCTTCTACAGCCTCCGCCGAGTGTA TGTGGTCAACAAGGAAATATGTGTCCGCACTGTCTGTGCCCACGAGGAACTTCTCCGAGCTGACCTGTGC CGAGACAAGT
Notes:	The probe template was PCR amplified from IMAGE:2088435 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2088435 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001133 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source