EMAGE mouse gene expression spatial database

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EMAGE:31179

Dmbt1 deleted in malignant brain tumors 1 ( MGI:106210)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_010081_11	euxassay_010081_12	euxassay_010081_13	euxassay_010081_14	euxassay_010081_15

euxassay_010081_16	euxassay_010081_17	euxassay_010081_18	euxassay_010081_19	euxassay_010081_20

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View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

hindgut

moderate

regional

moderate expression: see section 11 12 13 14 15 weak expression: see section 10

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T32098
Entity Detected:	Dmbt1, deleted in malignant brain tumors 1 ( MGI:106210)
Sequence:	sense strand is shown >T32098 TGGAGGTTTCCTGACCCAACCCTCTGGGCAATTTTCTAGCCCATTCTACCCTGGGAATTATCCTAATAAT GCCAGATGTCTGTGGAACATCGAGGTCCCAAACAACTACCGTGTGACTGTGGTCTTCAGAGATTTGCAGC TGGAAAGGGGCTGCAGCTATGATTATATAGAGATTTTTGATGGCCCCCACCACAGTTCACCTCTCATCGC CCGGGTTTGTGATGGGTCCCTGGGCTCTTTCACCTCAACATCCAACTTCATGTCAATTCGCTTCATCACT GATCACAGTATTACTGCGAGAGGGTTCCAGGCTCACTACTACTCCGACTTTGACAATAACACCACCAATC TCCTTTGTCAGTCAAATCACATGCAAGCCAGCGTGAGCAGGAGCTACCTTCAGTCCATGGGCTATTCTGC CAGGGATCTTGTCATTCCTGGTTGGAACTCCAGCTACCACTGTCAGCCCCAGATAACACAGAGGGAGGTC ATATTCACAATTCCCTACACAGGCTGTGGCACAATCAAACAGGCTGACAACGAGACCATCAACTACTCCA ACTTCCTCAGAGCGGTTGTTTCAAACGGCATCATCAAAAGGAGAAAGGACCTGAATATCCACGTCAGCTG CAAGATGCTGCAGAACACCTGGGTCAACACCATGTACATCACCAACAACACAGTGGAGATCCAGGAAGTT CAGTATGGCAATTTCGACGTAAACATTTCCTTCTACACATCCTCCTCCTTCTTGTTTCCAGTGACCAGCA GCCCATACTACGTGGATCTGGACCAGAATTTGTACCTTCAGGCCGAAATC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6396511), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58149. Forward Primer - name:058149_F_IRAV91_d10_Dmbt1, sequence:TGGAGGTTTCCTGACCCA; Reverse Primer - name:058149_R_SP6_IRAV91_d10_Dmbt1, sequence:GGATTTCGGCCTGAAGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_010081 same experiment
	EMAGE:29372 same embryo
	EMAGE:29403 same embryo
	EMAGE:29325 same embryo
	EMAGE:29405 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source