EMAGE mouse gene expression spatial database

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EMAGE:31204

Rbm47 RNA binding motif protein 47 ( MGI:2384294)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002217_01	euxassay_002217_02	euxassay_002217_03	euxassay_002217_04	euxassay_002217_05

euxassay_002217_06	euxassay_002217_07	euxassay_002217_08	euxassay_002217_09	euxassay_002217_10

euxassay_002217_11	euxassay_002217_12	euxassay_002217_13	euxassay_002217_14	euxassay_002217_15

euxassay_002217_16	euxassay_002217_17	euxassay_002217_18	euxassay_002217_19	euxassay_002217_20

euxassay_002217_21	euxassay_002217_22	euxassay_002217_23	euxassay_002217_24	euxassay_002217_25

euxassay_002217_26

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

strong

regional

strong expression: see section 12 13 14 15 16

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10

kidney calyx

strong

regional

strong expression: see section 06 16 18 moderate expression: see section 08 09 15 19

rectum

strong

regional

strong expression: see section 13

urethra of male

weak

regional

weak expression: see section 13 14

4th ventricle lateral recess

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midgut

strong

regional

strong expression: see section 11 12 13 14 15 17 18

bladder

strong

regional

strong expression: see section 13 14

pancreas

strong

regional

strong expression: see section 07 08 09 10 11 14 15 16

submandibular gland primordium

moderate

regional

moderate expression: see section 06 16 17 weak expression: see section 07 08 18 19

thymus primordium

strong

homogeneous

strong expression: see section 13 moderate expression: see section 09 10 11 12 14

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 14 17 18 moderate expression: see section 15

kidney pelvis

strong

regional

strong expression: see section 17 moderate expression: see section 07

adrenal gland

weak

regional

weak expression: see section 06 07 08 14 15 16

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 12 13 14 16 17 18 19 20

hindgut

strong

regional

strong expression: see section 12 13 14 15

lateral ventricle choroid plexus

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

lung

moderate

spotted

moderate expression: see section 02 03 04 05 06 07 08 09 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4579
Entity Detected:	Rbm47, RNA binding motif protein 47 ( MGI:2384294)
Sequence:	sense strand is shown >T4579 CCCACGCGTCCGGAGGACGGCAGGGCAGAGTGAGCACCAGGTGTTTGGCAGGGTTTGCCTCAGAGGACAC GTCAAGAACTGATCAGAGAAATCCCAAGCGAGCTGCCTGAACCTTCGAGAATTCTCTTGTTCTACCTTCC ATATAAAGAACCAGGACCAATCGCATTGGTGACCTTTGGTTTCCACCAGCTGTGGATGCCTTTGACAGTA TGACTGCTGAAGATTCCGCCACAGCCATGAACAGCGATCCAACAGTGGGGTCCTCCACCAAGGTACCGGA GGGTGTGGCGGGCGCGCCCAATGAGGCTGCACTGTTGGCACTGATAGAGCGCACCGGCTACACCATGGTA CAGGAGAACGGGCAGCGCAAGTACGGTGGACCCCCGCCCGGCTGGGAGGGCCCGCATCCCCAGCGTGGCT GTGAGGTCTTCGTGGGCAAGATCCCACGTGACGTGTATGAAGATGAGCTGGTGCCTGTGTTTGAGACCGT AGGTCGCATCTATGAGCTGCGACTCATGATGGATTTCGATGGCAAGAACCGCGGCTATGCCTTCGTGATG TACTGCCACAAGCATGAGGCCAAGCGAGCCGTTCGGGAACTCAACAACTACGAGATCCGCCCCGGCCGTC TGCTGGGTGTGTGCTGTAGTGTGGACAACTGCCGTCTCTTCATTGGTGGCATCCCCAAGATGAAGAAGCG CGGGGAGATCCTGGAGGAGATCGCCAAGGTCACCGAGGGCGTACTCAACGTGATTGTGTATGCCAGTGCG GCAGACAAGATGAAAAACCGAGGCTTCGCCTTTGTGGAGTATGAGAGCCATCGTGCTGCAGCCATGGCAC GCCGCAAGCTCATGCCGGGTCGGATCCAACTGTGGGGCCACCAGATTGCTGTGGACTGGGCCGAGCCAGA GATTGACGTGGACGAAGATGTGATGCAGACAGTGAAGATCCTCTACGTGCGCAACCTCATGATCGAGACC ACCGAGGAGACCATCAAGAAGAGCTTTGGCCAGTTCAACCCAGGCTGCGTGGAAAGGGTCAAGAAGATCC GTGACTACGCCTTCGTGCACTTCACTAGCCGTGAGGATGCTGTGCATGCTATGAACAACCTCAACGGCAC AGAGCTGGAGGGATCCTGCTTAGAAGTCACGCTGGCCAAGCCCGTGGACAAGGAACAGTACTCCCGCTAC CAGAAGGCAGCCAAGGGTGGTGGTGGCTCGGCTGAGGCCGTAGCGCAGCAACCCAGCTACGTGTACTCCT GTGACCCCTACACGTTGGCCTACTACGGCTACCCCTACAACGCTCTCATCGGGCCCAACCGGGACTACTT CGTGAAAACAGGCAGCATAAGAGGCCGAGGGAGAGGCGCAGCTGGAAACAGGACCCCAGGGCCCAGGGGT TCCTACCTCGGAGGATATTCCGCTGGCCGTGGTATATATAGCCGATATCACGAAGGCAAAGGCAAACAGC AGGAGAAAGGATATGAACTTGTGCCGAATTTGGAGATCTCTCCTGTCAATCCAGTTGCCATTAAACCTGG CACAGTGGCCATCCCTGCCATCGGTGCTCAGTACTCCATGTTCCAGGCAGCCCCAGCCCCGAAAATAATC GAAGATGGCAAGATCCACACAATGGAACACATGATCAGCCCCATTGCGGTGCAGCCTGACCCCGCCACTG CTGCTGCCGCTGCAGCCGCTGCAGCCGCTGCTGCCGTCATTCCTGCGGTATCCACACCGCCACCTTTCCA GGGCCGTCCAATAACTCCTGTGTACACCGTGGCACCAAATGTTCAGAGAATCCCCACCGCCGGCATCTAC GGGGCCAGCTACGTCCCCTTTGCTGCTCCCGCCACTGCCACAATCGCCACACTACAGAAGAACGCCGCGG CTGCCGTGTATGGGGGCTACGCCGGCTACATACCTCAGGCCTTCCCCGCTGCTCTCCAGGTGCCCATCCA CGACGTCTACCAGACTTACTGAGAACGGGGGACCGGAGCCAAGGCAAGCCACCAAACCCCGCTGAAGGAA CACTTTATTATTTATGAAGAAAGAACGTGTCGGGTTGGTACACGTGCAAAACCTGCAGGTGAAGAAGAAT GTTATCAAATAGCACACTGAAATATTTTATTATATCCATGAAGTTTTCACTCGCTTTTTTTTTTTTTTTT TTTTTGAAAATGACTTTTCAATTTAGCAGGCCCGCGTTCATACATTTCTAAAAGACTTGCGACGCCTCAC GCCTTAATGCCATCTCTTAAAGTTTGTATGCTCTTCTGCTTTTGTACAGAGGTTTGGTTTTTTGTTTTTT AAGGATATATTTTCAGTATGAAGGTTATTTTCTTAACTTCTGCACTCCAGAGTTTTCTATTTTGTAGAAC CTTTCAAAGTATATCAGCTATATGTATGTATATCAAAAAAAAAAAAAAAAAAAAGCACACCCGCGCATCT AGGGAACTATTTTGAAAACTATATTCAATATTTAAAGACACAAACTGTAGTGCTCTGAAATACTACATTA TTTTATTTTTTAAAGTGATGCTGGAAAGTGCAATTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4984936 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4984936 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002217 same experiment
	EMAGE:31725 same embryo
	EMAGE:29890 same embryo
	EMAGE:31476 same embryo
	EMAGE:31441 same embryo
	EMAGE:31865 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source