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EMAGE:31209

Slc22a6 ( MGI:892001)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31209 EMAGE:31209 EMAGE:31209 EMAGE:31209 EMAGE:31209
euxassay_012001_01 euxassay_012001_02 euxassay_012001_03 euxassay_012001_04 euxassay_012001_05
EMAGE:31209 EMAGE:31209 EMAGE:31209 EMAGE:31209 EMAGE:31209
euxassay_012001_06 euxassay_012001_07 euxassay_012001_08 euxassay_012001_09 euxassay_012001_10
EMAGE:31209 EMAGE:31209 EMAGE:31209 EMAGE:31209 EMAGE:31209
euxassay_012001_11 euxassay_012001_12 euxassay_012001_13 euxassay_012001_14 euxassay_012001_15
EMAGE:31209 EMAGE:31209 EMAGE:31209 EMAGE:31209 EMAGE:31209
euxassay_012001_16 euxassay_012001_17 euxassay_012001_18 euxassay_012001_19 euxassay_012001_20
EMAGE:31209 EMAGE:31209
euxassay_012001_21 euxassay_012001_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
hip
weak weak
regionalweak expression: see section 08 09 16
temporal bone petrous part
weak weak
regionalweak expression: see section 01 02 03 04 18 19 20 21 22
humerus
weak weak
regionalweak expression: see section 01 02 03 04 19 20 21 22
fibula
weak weak
regionalweak expression: see section 01 22
rib
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 14 15 16 17 18 19 20 21
axial skeleton
weak weak
regionalweak expression: see section 05 06 07 08 09 10 11 12 13 14
viscerocranium
weak weak
regionalweak expression: see section 08 09 10 18 19
otic capsule
weak weak
regionalweak expression: see section 07 08 09 15 16
mitral valve
weak weak
regionalweak expression: see section 12 13
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 04 05 06 08 09 10 11 12 13 14 17 weak expression: see section 07 15 16
basioccipital bone
weak weak
regionalweak expression: see section 08 09 10 11 12 13 14 16
basisphenoid bone
weak weak
regionalweak expression: see section 09 10 11 12 13 14 16
tibia
weak weak
regionalweak expression: see section 01 02 03 22
scapula
weak weak
regionalweak expression: see section 01 02 03 19
femur
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 18 19 20 22
nasal septum
weak weak
regionalweak expression: see section 12 13 14 15 16 17
exoccipital bone
weak weak
regionalweak expression: see section 01 02 03 04 18 19 20 21 22
orbito-sphenoid
weak weak
regionalweak expression: see section 01 02 03 04 06 07 08 09 18 19 20 21 22
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30072
Entity Detected:Slc22a6, ( MGI:892001)
Sequence:sense strand is shown

>T30072
GGAGCCATGATGTTTGGCTACCTGGCGGACAGGCTGGGCCGTCGGAAGGTGCTGATCTTGAACTACCTGC
AAACAGCTGTGTCGGGAACCTGTGCAGCCTATGCACCCAACTATACTGTCTACTGCATTTTCCGGCTCCT
CTCGGGCATGTCTTTGGCTAGCATTGCAATCAACTGCATGACACTAAACATGGAGTGGATGCCTATTCAC
ACCCGTGCCTATGTGGGCACCTTGATTGGCTATGTCTACAGCTTGGGCCAGTTCCTCCTGGCTGGTATCG
CCTATGCTGTGCCCCACTGGCGCCACCTGCAGCTTGCGGTCTCTGTGCCTTTTTTCGTTGCCTTCATCTA
CTCTTGGTTCTTCATTGAGTCAGCCCGCTGGTACTCCTCCTCTGGAAGGCTGGACCTCACCCTCCGAGCC
CTGCAGAGAGTGGCCCGCATCAACGGGAAACAAGAAGAGGGGGCTAAGCTGAGTATAGAGGTGCTCCAGA
CCAGCCTGCAGAAGGAACTGACTCTAAACAAAGGCCAGGCCTCCGCCATGGAGCTGCTGCGCTGCCCCAC
GCTTCGGCGCCTCTTCCTCTGCCTCTCTATGCTGTGGTTTGCCACTAGCTTTGCCTACTACGGGCTGGTC
ATGGACCTGCAGGGCTTTGGGGTCAGCATGTACCTTATCCAGGTGATTTTCGGCGCTGTGGACCTGCCTG
CCAAGTTTGTGTGCTTCCTAGTCATCAATTCCATGGGCCGCCGGCCTGCACAGTTGGCCTCCCTGCTGCT
GGCAGGCATCTGTATCCTAGTGAATGGCATAATACCGAGGGGCCATACAATCATTCGCACATCCCTGGCT
GTACTAGGGAAAGGCTGTCTGGCTTCCTCTTTCAACTGCATCTTCCTGTACACCGGAGAGCTGTACCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4163278), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17195. Forward Primer - name:017195_F_IRAV20-23_D22_Slc22a6, sequence:GGAGCCATGATGTTTGGC; Reverse Primer - name:017195_R_SP6_IRAV20-23_D22_Slc22a6, sequence:GGGGTACAGCTCTCCGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_012001 same experiment
 EMAGE:31495 same embryo
 EMAGE:31494 same embryo
 EMAGE:31890 same embryo
 EMAGE:31498 same embryo
 EMAGE:31497 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS