EMAGE mouse gene expression spatial database

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EMAGE:31248

Hsd17b4 ( MGI:105089)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_011373_01	euxassay_011373_02	euxassay_011373_03	euxassay_011373_04	euxassay_011373_05

euxassay_011373_06	euxassay_011373_07	euxassay_011373_08	euxassay_011373_09	euxassay_011373_10

euxassay_011373_11	euxassay_011373_12	euxassay_011373_13	euxassay_011373_14	euxassay_011373_15

euxassay_011373_16	euxassay_011373_17	euxassay_011373_18	euxassay_011373_19	euxassay_011373_20

euxassay_011373_21	euxassay_011373_22	euxassay_011373_23	euxassay_011373_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 5 mesenchyme

moderate

regional

moderate expression: see section 06 23 24

hindlimb digit 1 mesenchyme

moderate

regional

moderate expression: see section 06 24

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14 15

hindlimb digit 4 mesenchyme

moderate

regional

moderate expression: see section 04 06 23 24

pons ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

hindlimb digit 2 mesenchyme

moderate

regional

moderate expression: see section 06 24

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

spinal cord ventricular layer

moderate

regional

moderate expression: see section 14 15 16

hindlimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 04 06 23 24

medulla oblongata alar plate ventricular layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

vibrissa

moderate

regional

moderate expression: see section 05 06 07 08 21 22 23 24

telencephalon ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

upper lip

strong

regional

strong expression: see section 07 08 09 10 11 12 19 20 21 22 23

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14 15

lower lip

strong

regional

strong expression: see section 07 08 09 10 11 12 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30098
Entity Detected:	Hsd17b4, ( MGI:105089)
Sequence:	sense strand is shown >T30098 TGGATAAAGGCTCTGGCGTAGTGATTGTTATGGACGTCTATTCTTATTCTGGGAAGGAACTTATATGCTA TAATCAGTTCTCTGTCTTTGTTGTTGGCTCTGGGGGCTTTGGTGGAAAACGGACATCAGAAAAACTCAAA GCAGCTGTAGCTGTACCAAATCGACCTCCAGATGCTGTACTGAGAGATGCCACCTCACTGAATCAGGCCG CGCTGTACCGCCTCAGCGGAGACTGGAATCCTCTACACATTGACCCGGACTTTGCGAGCGTTGCCGGTTT TGAGAAGCCCATATTACATGGACTATGTACCTTTGGATTTTCTGCAAGGCATGTTTTACAGCAGTTTGCA GATAATGATGTATCAAGATTCAAGGCGATTAAGGTTCGTTTTGCCAAACCAGTGTATCCAGGACAGACTC TACAAACTGAGATGTGGAAGGAAGGAAACAGAATTCATTTTCAAACCAAGGTCCACGAGACTGGAGATGT TGTCATTTCAAATGCGTACGTGGATCTCGTGCCTGCATCTGGAGTTTCAACCCAGACACCTTCAGAGGGT GGAGAGCTCCAGAGTGCTCTTGTGTTTGGGGAGATAGGCCGCCGCCTCAAGAGTGTTGGCCGTGAGGTGG TAAAGAAAGCGAATGCTGTGTTTGAATGGCATATCACGAAAGGTGGGACTGTTGCAGCCAAGTGGACCAT TGACCTGAAGAGCGGCTCAGGGGAGGTGTACCAAGGCCCCGCAAAGGGCTCTGCTGATGTGACCATCATC ATTTCCGATGAGGATTTTATGGAAGTGGTCTTCGGCAAGCTTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4209394), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17235. Forward Primer - name:017235_F_IRAV20-23_N06_Hsd17b4, sequence:TGGATAAAGGCTCTGGCG; Reverse Primer - name:017235_R_SP6_IRAV20-23_N06_Hsd17b4, sequence:TCAAGCTTGCCGAAGAC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_011373 same experiment
	EMAGE:31275 same embryo
	EMAGE:29357 same embryo
	EMAGE:31210 same embryo
	EMAGE:31122 same embryo
	EMAGE:29348 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source