EMAGE mouse gene expression spatial database

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EMAGE:31259

Anpep ( MGI:96749)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004850_01	euxassay_004850_02	euxassay_004850_03	euxassay_004850_04	euxassay_004850_05

euxassay_004850_06	euxassay_004850_07	euxassay_004850_08	euxassay_004850_09	euxassay_004850_10

euxassay_004850_11	euxassay_004850_12	euxassay_004850_13	euxassay_004850_14	euxassay_004850_15

euxassay_004850_16	euxassay_004850_17	euxassay_004850_18	euxassay_004850_19	euxassay_004850_20

euxassay_004850_21	euxassay_004850_22	euxassay_004850_23	euxassay_004850_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

mandible

strong

regional

strong expression: see section 03 04 05 06 07 08 09 16 17 18 19 20 21 moderate expression: see section 14 15

liver right lobe

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 20 21 22 23

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain meninges

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17

stomach

strong

regional

strong expression: see section 03 04 05 06 07 08

kidney calyx

strong

regional

strong expression: see section 05 06 07 08 09 13 14 15 16 17

diencephalon meninges

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

rectum

strong

regional

strong expression: see section 11

telencephalon meninges

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 18 19 20 21 22 23 24

midgut

strong

regional

strong expression: see section 10 11 12 13 14 15 16

midgut loop

strong

regional

strong expression: see section 06 07 08 09 10 14 15

hindbrain meninges

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

renal cortex

strong

regional

strong expression: see section 05 06 07 08 09 13 14 15 16 17

spinal cord meninges

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 not examined expression: see section 05

hindgut

strong

regional

strong expression: see section 11

maxilla

strong

regional

strong expression: see section 04 05 06 07 08 09 16 17 18 19 20 21 moderate expression: see section 14 15

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6565
Entity Detected:	Anpep, ( MGI:96749)
Sequence:	sense strand is shown >T6565 CTGGCCCCGGGGCTGCTGTTCTTTCCTCTTGGCCTGAGCTATTCCGAGCTCCCTGTCCACCGGCATCATG GCCAAGGGGTTCTACATTTCCAAGACCCTGGGCATCTTGGGCATCCTGTTGGGTGTGGCAGCTGTGTGTA CCATCATAGCTCTGTCGGTGGTCTACGCTCAGGAGAAGAATAGGAATGCAGAGAACTCTGCCACAGCCCC CACGCTCCCGGGCAGCACCTCAGCCACCACCGCAACCACCACCCCTGCTGTAGATGAAAGCAAGCCTTGG AACCAGTATCGCTTGCCTAAGACTCTTATACCTGACTCCTACCGGGTGATCCTGAGACCCTACCTCACCC CCAACAATCAGGGCCTGTACATCTTCCAAGGCAACAGTACTGTTCGCTTTACCTGCAACCAGACCACGGA TGTCATTATCATCCACAGCAAAAAGCTCAACTACACCCTCAAAGGAAACCACAGGGTGGTGTTGCGAACC CTGGACGGCACTCCGGCACCTAACATTGACAAAACGGAACTGGTAGAGCGTACTGAGTACCTGGTGGTGC ACCTGCAGGGGTCCCTGGTAGAGGGCCGTCAGTACGAGATGGACAGCCAGTTCCAGGGGGAACTGGCTGA TGACCTGGCTGGCTTCTACCGCAGCGAGTACATGGAAGGAGACGTCAAGAAAGTGGTGGCTACAACGCAG ATGCAGGCTGCTGATGCTCGGAAATCCTTTCCATGTTTTGATGAGCCAGCCATGAAGGCCATGTTCAACA TCACACTCATCTACCCCAACAACCTCATAGCTCTGTCTAATATGCTTCCCAAAGAGTCCAAGCCCTATCC GGAAGACCCTTCCTGCACCATGACTGAGTTCCACTCCACCCCTAAGATGTCCACATACCTGCTGGCCTAC ATCGTGAGCGAGTTCAAAAATATAAGCTCCGTCTCAGCCAATGGTGTCCAGATTGGAATCTGGGCTCGGC CCAGTGCCATTGATGAGGGCCAGGGTGATTACGCACTGAACGTTACAGGCCCCATCCTAAATTTCTTTGC CCAACATTATAATACATCCTACCCTCTACCAAAGTCTGACCAGATTGCCCTGCCTGACTTCAACGCTGGA GCCATGGAGAACTGGGGTCTGGTGACCTACCGTGAGAGCTCCCTGGTCTTTGACTCTCAGTCCTCCTCCA TTAGCAACAAGGAGCGGGTGGTCACTGTGATTGCTCACGAGCTGGCCCATCAGTGGTTTGGCAACCTGGT GACTGTGGCTTGGTGGAATGATCTGTGGCTGAACGAGGGCTTTGCCTCCTACGTGGAATATCTGGGTGCT GACTATGCAGAGCCTACCTGGAATCTGAAAGACCTCATGGTACTGAATGATGTGTACCGTGTGATGGCCG TGGATGCCCTTGCCTCCTCCCACCCACTGTCCAGTCCTGCTGACGAGATCAAAACACCAGACCAGATCAT GGAGCTGTTTGACAGCATCACCTACAGCAAGGGAGCCTCAGTCATCAGGATGCTGTCCAGTTTCCTGACA GAGGACCTGTTTAAGAAGGGCCTTTCATCTTATCTCCACACCTACCAGTACTCGAACACCGTTTATCTGG ACCTGTGGGAACACCTGCAAAAGGCCGTGAACCAACAGACAGCTGTCCAACCCCCGGCCACGGTGCGCAC TATCATGGACCGCTGGATTCTACAGATGGGCTTTCCCGTTATCACTGTGAACACCAATACAGGAGAAATC TCCCAGAAACACTTCCTCCTGGATTCCAAGTCCAACGTTACCCGCCCCTCCGAGTTTAATTACATCTGGA TCGCGCCCATTCCATTTCTCAAAAGTGGACAGGAGGATCACTACTGGCTGGATGTCGAGAAAAACCAGAG TGCAAAGTTCCAGACATCCTCCAATGAATGGATCTTACTGAACATTAACGTAACCGGCTACTACCTGGTT AACTATGATGAGAACAACTGGAAGAAGCTTCAGAATCAGCTGCAAACAGACCTTTCTGTTATCCCTGTCA TCAACCGAGCACAGATTATCCACGACTCCTTCAACCTGGCCAGTGCTAAAATGATACCCATCACCCTGGC GCTGGACAACACCCTCTTCCTGGTCAAAGAGGCGGAGTACATGCCCTGGCAGGCTGCCCTGAGCAGCCTC AACTACTTCACACTCATGTTCGACCGCTCGGAGGTCTACGGCCCCATGAAGAGGTATCTGAAGAAGCAAG TTACGCCCCTCTTCTTCTACTTCCAAAATAGAACCAACAACTGGGTCAACCGTCCTCCAACGCTGATGGA GCAGTACAATGAAATTAACGCCATCAGCACCGCCTGTTCCAGTGGTCTCAAAGAGTGTAGGGACCTGGTC GTTGAGCTCTATAGTCAGTGGATGAAAAACCCTAATAATAACACGATCCACCCCAACCTTCGGTCTACTG TCTACTGCAATGCCATTGCTTTCGGTGGCGAAGAAGAGTGGAACTTTGCTTGGGAACAGTTCCGGAATGC AACTCTGGTGAACGAAGCGGACAAACTCCGGTCAGCCTTGGCCTGTAGCAAAGATGTGTGGATTTTGAAC AGGTACCTGAGTTACACTCTGAACCCGGACTACATCCGGAAGCAGGACACCACCTCCACCATCATCAGCA TTGCCAGCAACGTGGCTGGGCACCCTCTGGTTTGGGACTTTGTCCGAAGCAACTGGAAGAAACTGTTTGA GAATTACGGTGGAGGATCTTTCTCCTTTGCCAATCTCATCCAGGGAGTGACCCGGCGCTTCTCCTCTGAG TTCGAGCTGCAGCAGCTGGAGCAGTTTAAAGCGGATAACTCAGCCACAGGCTTTGGCACCGGCACTCGGG CTCTGGAGCAAGCCCTGGAGAAGACGAGAGCCAACATCGACTGGGTGAAGGAGAACAAAGATGCGGTATT CAAGTGGTTCACAGAGAACAGCAGTTAGTTCCTGGTTCTGAGAACCACTTGTCCCAGTATGACACCTCTT ACTATCTCAGCAGCCTGTGCAGGGTCTCTGTCCTCAGAGCTCCAGACACCAGCATCCTACTCTCAAGGAT GAAGTCTCCAGCCTGTGGAGCCAGCCTAGCTCCTAACTGTCAGGCTGACGGACACCTCCCAGGTCTTGCA CCCTCATGCCAACTCTGCCCCAGGTCCAGGCCTCTGGGGCTGATCTCAGGGAAGCCCAGCTCTGAAGCTA GATTTACTGGACAAAGGGCAGCCTGGAAAGAGACTCCCTGAATGCTTTACTATCCCTGCCCCCTACCCCC ACCCCTACCCCCCACGAGATCCAGAACCAAAGAATCAACAGGGCACAAGATCTATATATATTTTTAAGAG AAAATGTAAATAAAGAATTTCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3593307 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3593307 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004850 same experiment
	EMAGE:30835 same embryo
	EMAGE:30228 same embryo
	EMAGE:30254 same embryo
	EMAGE:31243 same embryo
	EMAGE:29349 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source