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EMAGE:31265

Ephb4 Eph receptor B4 ( MGI:104757)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31265 EMAGE:31265 EMAGE:31265 EMAGE:31265 EMAGE:31265
euxassay_000222_01 euxassay_000222_02 euxassay_000222_03 euxassay_000222_04 euxassay_000222_05
EMAGE:31265 EMAGE:31265 EMAGE:31265 EMAGE:31265 EMAGE:31265
euxassay_000222_06 euxassay_000222_07 euxassay_000222_08 euxassay_000222_09 euxassay_000222_10
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euxassay_000222_11 euxassay_000222_12 euxassay_000222_13 euxassay_000222_14 euxassay_000222_15
EMAGE:31265 EMAGE:31265 EMAGE:31265 EMAGE:31265 EMAGE:31265
euxassay_000222_16 euxassay_000222_17 euxassay_000222_18 euxassay_000222_19 euxassay_000222_20
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euxassay_000222_21 euxassay_000222_22 euxassay_000222_23 euxassay_000222_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
mesenchyme
moderate moderate
single cellmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T428
Entity Detected:Ephb4, Eph receptor B4 ( MGI:104757)
Sequence:sense strand is shown

>T428
GTTAAATAATATGTAAAATGAAAAAAAAAAAATCTCCAGATGATGTCTGGCCAGGCAGGTCTTCTGGTCC
CTCCTCAGTATCTTCACTGAGCCAAAGTGCTCCCTAATTTAAGGCCTAGTTCTTTACCTGTGTGGTCCCT
GTCTGTATTTCTTTCTCCTCCAAGGCAACCTCTGCAGCTTCTTCCCCCTAAAATCTCTCTGAGGACTCTC
TCTTTCCTTCTTCTACGGAGGCCGCTCAGTAAATGTTTGGTGATTACTCTTGCCTTTCTCCTCCTGTGGC
CTCCCTGCTTGGCTGGTCCCGGGACTCCTCCAACCTTCTGTTTTTCCACAGGGACCTTCTCCGAATTGGA
GTCACTCTGGCAGGACACCAGAAGAAAATCTTGGCCAGTGTGCAGCATATGAAGTCCCAAGCTAAGCCAG
GAGCCCCTGGTGGGACAGGGGGACCAGCCCAGCAGTTCTGACCTCCAAGGACTCACCACCGTGGCAGATT
CTTCTTT
Notes:The probe template was PCR amplified from IMAGE:3158277 using vector specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3158277 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000222 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS