EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31266

Scoc short coiled-coil protein ( MGI:1927654)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002885_01	euxassay_002885_02	euxassay_002885_03	euxassay_002885_04	euxassay_002885_05

euxassay_002885_06	euxassay_002885_07	euxassay_002885_08	euxassay_002885_09	euxassay_002885_10

euxassay_002885_11	euxassay_002885_12	euxassay_002885_13	euxassay_002885_14	euxassay_002885_15

euxassay_002885_16	euxassay_002885_17	euxassay_002885_18	euxassay_002885_19	euxassay_002885_20

euxassay_002885_21	euxassay_002885_22	euxassay_002885_23	euxassay_002885_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 13 18 19 20

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 07 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 09 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 18 19 20 21 22 23 24

cervical ganglion

moderate

regional

moderate expression: see section 10 18

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 12 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 19 20

thoracic ganglion

moderate

regional

moderate expression: see section 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1092
Entity Detected:	Scoc, short coiled-coil protein ( MGI:1927654)
Sequence:	sense strand is shown >T1092 GTTGGCCTACTGGTTTGCTCATGAGCAAGATGGACGGATTGAGGACAGGGGAGGAGGAAGACAGCACGTT CACCAGCATTTCTCTTGAAGATGACACAGACCATTCTTTAAAGAGTTGGCGTTCGAGGGCTGAAAGTCTG CTACCCAAGATGATGAATGCTGACATGGACGCAGTTGATGCTGAAAATCAGGTGGAACTGGAAGAAAAGA CTCGACTTATTAATCAGGTGTTGGAACTCCAACACACACTTGAAGATCTTTCTGCAAGAGTAGATGCAGT TAAGGAAGAAAATCTGAAGCTAAAGTCGGAAAATCAAGTTCTCGGACAATATATAGAAAACCTCATGTCT GCTTCTAGTGTTTTTCAAACAACTGATACAAAAAGCAAAAGGAAGTAAAGGGCTCACCTCCCTCTCTTCT ATGGATTGCTGCTTTTTTTCTTCCCCCTTAAGGCTTGGATATGATCCAAAAGTTAGAGTATCTTTGTTCT TCCCTGAGTATTTATAAAGAGAATGTCACATCTAGGTAACACTAATGCTGAACAGGAGCATCTCTGAGTT AAC
Notes:	The probe template was PCR amplified from IMAGE:2123211 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2123211 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002885 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source