EMAGE mouse gene expression spatial database

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EMAGE:31275

Ash2l ash2 (absent, small, or homeotic)-like (Drosophila) ( MGI:1344416)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

lower jaw molar

strong

regional

strong expression: see section 08 20

vomeronasal organ

moderate

regional

moderate expression: see section 13 16 17

upper jaw molar

strong

regional

strong expression: see section 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 15 16 17 18 19 20

seminiferous cord

moderate

regional

moderate expression: see section 07 08 09 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30100
Entity Detected:	Ash2l, ash2 (absent, small, or homeotic)-like (Drosophila) ( MGI:1344416)
Sequence:	sense strand is shown >T30100 GAGCTGCACGGTTTCCATTAACTTTGGACCATCCTTCAAATACCCTCCAAAGGATCTCACGTACCACCCT ATGAGTGACATGGGCTGGGGCGCTGTGGTAGAACACACACTGGCTGATGTTTTGTATCACGTGGAGACAG AAGTGGACGGAAGACGTAGTCCACCCTGGGAACCCTAACCAGTCCTTGCTTCTGGTGACACTGTAAAATA ATCCTGGGGGTTTTGTTTTTGTTTTTATAAAGTGTCAAATGTTTTCCAAGAGATGCTGCAGTACACTTTG ATCAAGGTAAAAGGGTGGGTGGGACTGCAGCAGTGCCTGCTCTTCAGCATCCCTGCCCCACACAACTACT TACTCTGAGTGCCATAAATCAGCCGCTCCCTGTAAGGCTGCTGCTGTGCCCAGACCTGCCAGGGAGGACC TGTTACTTTCCTGTGTCTGTGTGTTCCAAAGAGCGAGTAATCATGCTCAGACAGCTTGCTTGAGGATGTT GGATCCCCTGGTTTCTGGGGAGGCAAGCTTGTTTGAGTGGCTGAAATCAATGGCATTCCATTCCAGAGCA GTCTAAAGGCAGTCTTTTTGGTGTGGAGTTTGGGGATCATCTGGGAAGCTAGTGGGTTCTATAAACTATA ATTGTGAAAGGAATATGCCTACAGGGTTTAAAAGATAGATCTTTCCTCCAATAAAACTTGTTTTCAAGCT TGTATGGTACTTGGCTTCACTGGAAGTGAAGTTCCTGTTGACCCTGTTAGCAGTGTGAGCAGTGTGACGG GGACAACACTGGCAGGGTGGGACAGGCGCTTTTATTGCTGTCCTCCCCGGGTCACCAGGCGCTTTCACTG CCCTGTGTACAGTGGACTCTCAGGGAGAGCAAGACTGTAGCAGGCCTCCAGTCCGTGTCTTGGAACTTGG TGCTGATATGTGCGGCTTGTCCTCTTGCCTGCCTTTCTAGTAGCCAGCCAGGGGTTAGGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4211433), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 16575. Forward Primer - name:016575_F_IRAV20-23_N18_Ash2l, sequence:GAGCTGCACGGTTTCCAT; Reverse Primer - name:016575_R_SP6_IRAV20-23_N18_Ash2l, sequence:ATCCTAACCCCTGGCTG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_011361 same experiment
	EMAGE:29357 same embryo
	EMAGE:31210 same embryo
	EMAGE:31122 same embryo
	EMAGE:29348 same embryo
	EMAGE:31248 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source