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EMAGE:31283

Gm1582 ( MGI:2685472)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_013785_21 euxassay_013785_22 euxassay_013785_23 euxassay_013785_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
neural retina
strong strong
regionalstrong expression: see section 02 03 04 05 23 moderate expression: see section 24
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 03 05 06 07 16 17
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 09
spinal cord
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16
brain
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 04 05 06 17
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 11 12
retina
strong strong
regionalstrong expression: see section 01
dorsal root ganglion
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
facial vii ganglion
strong strong
regionalstrong expression: see section 03 05 06 19 20 21
vagus x ganglion
strong strong
regionalstrong expression: see section 07 16
trigeminal v ganglion
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 17 18 19 20 21 22
cervical ganglion
moderate moderate
regionalmoderate expression: see section 08 09 16
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37852
Entity Detected:Gm1582, ( MGI:2685472)
Sequence:sense strand is shown

>T37852
GTTCTCTGGGTGCTCTCCTAAAGTCTTCACTTCCAGAAGAATCCCAAGGGAGCGAGAGTGACTGGGAGGA
CCTGGACGAGCCCGTGGATAGGGATGAGGTGCTCAGGTGGACACGCTCAGCGAGACAGCCTCTTGCGGCA
GAACCCTGAGGGTGTCGGCATGGACATCCTTTTGGTGGAAGAGCCCTCAGCTGATTTTCATCCAGAAAAT
GTACTTGCTTGCTCTGTACTCTGAAAGAAGCATCGTCAAGACAATGGGCTGGGGTGGAGCTCCTGGGCTA
GCTATGAAGTGCTAACTTCTAAGAGTTTGTGCCTGTTACTTCTGAGGTTCGTTATCATTAGTAGCTACAA
CTTCCTCTTCAGTTCTGAAGTTTAAGTCAAGCAAATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGGAGTG
GGGTCCCCCTCATGACCTAAACTGGGAACTAATTTCTTTAGGTGAGATAAGCTGGAGCAGGGAAAGTATC
TGGCTGCTACACTGGGGCACCATTTTCCAAGATGGATGCCACAGTCTGGTTTAACTGTTGATTCCTCATT
GGTCAAAGGACTCTGTGCTTATCCAAGTGTCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 84581. Forward Primer - name:084581_F_cDNA_LOC380890, sequence:GTTCTCTGGGTGCTCTCCTAAA; Reverse Primer - name:084581_N_SP6_cDNA_LOC380890, sequence:GAGACACTTGGATAAGCACAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_013785 same experiment
 EMAGE:31284 same assay
 EMAGE:31282 same embryo
 EMAGE:31280 same embryo
 EMAGE:30305 same embryo
 EMAGE:31284 same embryo
 EMAGE:31277 same embryo
 EMAGE:31303 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS