EMAGE mouse gene expression spatial database

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EMAGE:31286

E130303B06Rik ( MGI:2142593)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_009274_01	euxassay_009274_02	euxassay_009274_03	euxassay_009274_04	euxassay_009274_05

euxassay_009274_06	euxassay_009274_07	euxassay_009274_08	euxassay_009274_09	euxassay_009274_10

euxassay_009274_11	euxassay_009274_12	euxassay_009274_13	euxassay_009274_14	euxassay_009274_15

euxassay_009274_16	euxassay_009274_17	euxassay_009274_18	euxassay_009274_19	euxassay_009274_20

euxassay_009274_21	euxassay_009274_22	euxassay_009274_23	euxassay_009274_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

weak

regional

weak expression: see section 01 02 03 22 23 24

not examined

regional

not examined expression: see section 08

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22

midbrain ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 08 09 10 14 15 16 17

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 09 10 11 15 16

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 10 11 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2439
Entity Detected:	E130303B06Rik, ( MGI:2142593)
Sequence:	sense strand is shown >T2439 TGGCTCGAGCAGATTCGGACGAGGAAANCTGCTCCAGGCTGCGGTAGGATCATGTGCGAGGGCCCGTCCC GCATCTCTGGACCCATCCCCCCAGACCCCACTCTCTGCCCGGACTACTACCGGCGGCCGGCCTCGGCCCA AGGACGCCTGGAAGGAAACGCGTTGAAGCTGGACCTGCTGACTTCAGGTCGGGACCTGGACTCCAGTCCT CCTCGTGGCCCTCGCATCAGGCCTGAAGCCCGAGAGATCCTAGAGCGTGGCCAGCGAGGCGTGGGGGACG TGCTGCTGCAACTGGGGTGCATCTCCCTCGGTTCGGGGGTCTCTCCTAAGAGGAAGAATCCAAAGGACCA TGAGAAGGAAAACTTGAGGCGGATCAAAGAGATTCAGAGGCGCTTCCAAGACCAGGAACGCAGCCGGGAA CAGGGTCAGCCTAAGCCACTGAAGGCACTGTGGCGCTCACCCAAGTATGACAATGTGGAGTCTCGAGTCA AGGCCAGGATGAAGGAGCTTGGCCCCACCTCTGTGACAGAACCTGCCCACTTTCTGCGGGCACAC
Notes:	The probe template was PCR amplified from IMAGE:1245862 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1245862 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009274 same experiment
	EMAGE:30549 same embryo
	EMAGE:30571 same embryo
	EMAGE:29311 same embryo
	EMAGE:29323 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source