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EMAGE:31289

Cpm carboxypeptidase M ( MGI:1917824)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_012219_01 euxassay_012219_02 euxassay_012219_03 euxassay_012219_04 euxassay_012219_05
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euxassay_012219_11 euxassay_012219_12 euxassay_012219_13 euxassay_012219_14 euxassay_012219_15
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euxassay_012219_16 euxassay_012219_17 euxassay_012219_18 euxassay_012219_19 euxassay_012219_20
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euxassay_012219_21 euxassay_012219_22 euxassay_012219_23 euxassay_012219_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
pelvic girdle musculature
weak weak
regionalweak expression: see section 11 18 19
cornea
weak weak
regionalweak expression: see section 03 04 23 24
stomach
moderate moderate
regionalmoderate expression: see section 07 08 09 weak expression: see section 04 05 06
humerus
weak weak
regionalweak expression: see section 03 04 05 20 21 22
hindlimb digit 4 mesenchyme
weak weak
regionalweak expression: see section 04 21
fibula
weak weak
regionalweak expression: see section 02 03
rib
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23 24
tarsus
weak weak
regionalweak expression: see section 04 05 22
hindlimb digit 2 mesenchyme
weak weak
regionalweak expression: see section 04 21
midgut
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 weak expression: see section 06 14 15 16 17
submandibular gland primordium
strong strong
regionalstrong expression: see section 07 08 09 10 17 18 19
hindlimb digit 3 mesenchyme
weak weak
regionalweak expression: see section 04 21
thymus primordium
moderate moderate
regionalmoderate expression: see section 11 14 15 weak expression: see section 12 13
tibia
weak weak
regionalweak expression: see section 02 03 24
femur
weak weak
regionalweak expression: see section 07 08 09 20 21
vibrissa
weak weak
regionalweak expression: see section 06 21 22
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 09 10 11 12 15 16 17 18 19 moderate expression: see section 08
hindgut
weak weak
regionalweak expression: see section 14
naris
strong strong
regionalstrong expression: see section 13 15 16
glans of male genital tubercle
moderate moderate
regionalmoderate expression: see section 13 14 15 16
diencephalon lateral wall ventricular layer
strong strong
regionalstrong expression: see section 12 13 14 15
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37643
Entity Detected:Cpm, carboxypeptidase M ( MGI:1917824)
Sequence:sense strand is shown

>T37643
AGTGGAGGTCACTGCTTAGCTCATGCTGCCCACACAAACGCCACCCCGAAGGAGTCCCAAACACTTGTAA
GAAAGTTCAGAAGCAAAACAGATATCCCTACAAGAAAAACAATTTGTCTACGGAGAGCCACGTGGATCAG
AGCCGCAAGGACGGGCACTTTCTGGTCACTTGCTGTGTTACGACCTTTCGTGTTATTTGTAGAGAGCTGA
TATTGTCTATAAGTGGTTCTCCCCAAAGGAAGAAATTTCTAGATAACGGGACTAAGAGCATTTACTCTTG
CCAATGGTCTAAGGCCAAATGAGAGAAAATGGACAAACATGTCTGATGTGGGTACAGATGCAGTGGCCTC
TGTCTCCCGTGCCTTTGCCCTTGCCCCCTGCACCTGATGCCACAGTCCTACGAAGATGACCCCTCCTGTA
TAACCCCAGTCCCTTGCCTGAGCCTCAGATGCAACTCCAGCTGCTCACCAGACACACCCACCTGACTACG
ATGCCTAGGAACTGCAGTTCATCCGGTTGACTGTTGAGACGCCATGCAAGGCTGTTCGATTCCCATATCC
AGTCACTGGAAGCTGCTGTATACAGCCTCTCCAGGCCAGTTAGCGTCCCTCCGCCGCTGACCCAGCTGCT
GCTCATGGGTATTTTCGCCAAGTCCGTCTTCCACACAGAACCTGGGGAGCTGGGCTGCCCAAAGCTCTCC
TGCTCCCTAAACACAACACAGTAAGTGCTCTCGGTCCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 82916. Forward Primer - name:082916_F_cDNA_Cpm, sequence:AGTGGAGGTCACTGCTTAGCTC; Reverse Primer - name:082916_N_SP6_cDNA_Cpm, sequence:GGGACCGAGAGCACTTACTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_012219 same experiment
 EMAGE:31208 same embryo
 EMAGE:31301 same embryo
 EMAGE:31232 same embryo
 EMAGE:31206 same embryo
 EMAGE:29459 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS