EMAGE mouse gene expression spatial database

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EMAGE:31303

Mcm4 minichromosome maintenance deficient 4 homolog (S. cerevisiae) ( MGI:103199)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_013775_06	euxassay_013775_07	euxassay_013775_08	euxassay_013775_09	euxassay_013775_10

euxassay_013775_11	euxassay_013775_12	euxassay_013775_13	euxassay_013775_14	euxassay_013775_15

euxassay_013775_16	euxassay_013775_17	euxassay_013775_18	euxassay_013775_19	euxassay_013775_20

euxassay_013775_21	euxassay_013775_22	euxassay_013775_23	euxassay_013775_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6425
Entity Detected:	Mcm4, minichromosome maintenance deficient 4 homolog (S. cerevisiae) ( MGI:103199)
Sequence:	sense strand is shown >T6425 GCGGCTGGCGGGAATCGCTTCACCCTGCGGGCCCAGGAACGTTCTCCTGGGTGGCGCAGAACCCTGACTC GCCGTTTTGTGCGCGTTTGCAACTAACCCGGATCCGAGGTACGAGAGGTCCCGGAGCGGGACAACTGAGA GCCAGATTTCTTTTGTCGCGCAGCAACCACAGCACCATGTCGTCCCCGGCATCCACCCCGAGCCGCCGCA GCAGCCGACGCGGACGAGTCACCCCAACCCAGTCCCTTCGAAGTGAGGAAAGCAGGTCGTCACCCAATCG GAGACGTAGAGGCGAAGATTCTTCCACCGGAGAGCTACTGCCAATGCCCACCTCACCAGGAGCCGACCTG CAGAGCCCACCTGCACAGAATGCCTTGTTTTCCAGCCCTCCTCAGATGCATTCTTTAGCTATTCCTTTGG ACTTTGATGTTAGTTCACCATTGACATATGGCACTCCCAGCTCGCGAGTGGAAGGAACCCCAAGAAGTGG GGTGAGAGGCACACCTGTAAGGCAGAGGCCAGATCTGGGCTCAGCACGAAAGGGTTTGCAGGTGGATCTG CAGTCTGATGGCGCAGCAGCAGAAGACATCGTACCAAGTGAACAGTCTCTAGGCCAAAAGCTTGTGATTT GGGGAACAGATGTGAATGTGGCAACATGTAAAGAGAATTTTCAGAGATTCCTTCAGTGTTTTACTGATCC TCTGGCCAAAGAAGAAGAAAATGTTGGCATAGATATTACTCAACCTTTGTACATGCAACAACTTGGAGAG ATTAATATTACAGGAGAGCCATTTTTAAATGTGAACTGCGAACACATAAAATCATTTAGCAAAAATCTGT ATAGACAGCTCATCTCCTACCCACAGGAGGTTATACCAACCTTTGACATGGCTGTCAATGAGATCTTCTT TGACCGTTATCCTGACTCCATCTTAGAACATCAGATTCAAGTCAGACCTTTTAATGCGTTGAAGACAAAG AGTATGAGAAACTTGAATCCAGAAGACATTGATCAGCTCATCACCATCAGTGGCATGGTCATCAGAACAT CACAGCTGATTCCGGAGATGCAGGAGGCCTTTTTCCAATGCCAAGTCTCTGCCCACACCACCCGGGTGGA GATAGATCGAGGCAGAATTGCTGAGCCCTGCAGTTGTGTGCACTGCCACACTACCCACAGCATGGCACTG ATCCACAACCGATCATTCTTCTCTGACAAGCAAATGATCAAACTTCAAGAGTCTCCTGAAGACATGCCTG CTGGGCAGACACCTCACACTATTGTCCTTTTTGCCCACAATGACCTTGTTGACAAGGTTCAACCAGGGGA CAGAGTGAACGTCACAGGCATATATCGAGCAGTACCAATTCGAGTTAATCCAAGAGTGAGCAACGTGAAG TCTGTCTATAAAACCCACATTGATGTCATTCATTATCGGAAAACGGATGCAAAACGTCTGCATGGCCTTG ATGAAGAAGCAGAACAGAAACTTTTTTCAGAGAAACGTGTGAAATTGCTTAAGGAACTTTCCAGGAAGCC AGATATTTATGAGCGGCTTGCTTCAGCCTTGGCTCCCAGCATTTATGAACATGAAGATATCAAAAAGGGA ATCTTACTTCAGCTCTTTGGTGGAACAAGGAAGGATTTCAGTCACACTGGGAGGGGTAAATTCCGTGCTG AGATCAACATCCTTCTGTGTGGGGACCCTGGCACCAGCAAGTCCCAGCTGCTACAGTATGTGTACAACCT GGTCCCCAGAGGCCAGTACACGTCTGGAAAAGGCTCCAGTGCGGTCGGCCTCACCGCCTATGTGATGAAA GACCCTGAGACCAGGCAGCTTGTCCTCCAGACAGGTGCCCTCGTCCTGAGTGACAATGGGATATGCTGCA TCGATGAGTTTGACAAAATGAATGAAAGCACAAGGTCTGTGCTGCATGAGGTCATGGAACAGCAGACTCT GTCCATTGCAAAGGCTGGGATCATCTGTCAGCTCAATGCGCGCACCTCTGTCCTGGCAGCAGCAAATCCT ATTGAGTCTCAGTGGAATCCTAAAAAAACAACCATTGAAAATATCCAACTACCGCACACATTGTTGTCAA GGTTTGATCTCATTTTCCTCATGCTAGACCCTCAGGATGAGGCATATGACCGGCGTCTAGCTCATCACCT GGTTTCATTGTACTACCAAAGTGAGGAGCAAGTGGAGGAGGAGTTCCTGGACATGGCCGTGCTGAAAGAC TACATTGCATATGCCCATAGTACCATCATGCCCCGACTGAGTGAGGAGGCCAGCCAGGCTCTCATTGAGG CTTATGTAAACATGAGGAAGATTGGGAGTAGCCGGGGGATGGTTTCTGCTTACCCTCGACAGCTAGAGTC ATTAATTCGCTTAGCAGAAGCCCATGCTAAAGTAAGATTTTCAAACAAAGTTGAAGCAATTGATGTGGAA GAGGCAAAACGCCTCCACCGGGAGGCTCTGAAGCAGTCTGCAACTGACCCTCGTACTGGCATTGTGGATA TTTCTATTCTTACTACAGGAATGAGTGCCACTTCTCGTAAACGGAAAGAAGAATTAGCTGAAGCATTGAG AAAACTTATTTTATCTAAGGGTAAAACACCAGCCTTAAAGTACCAACAGCTGTTTGAGGATATTCGGGGA CAGTCTGACACAGCAATTACCAAGGACATGTTTGAAGAAGCCCTGCGAGCTTTGGCTGATGATGATTTCC TAACAGTGACTGGGAAGACTGTCCGCCTGCTCTGAGCTGCATGGCCCTCGGACTAGAGTCACTCACTGTT GTTATTGCTGCAAAAGGAGTCTGAAAGGCAGTATTCCACTATATAAACCTCTTCTATTCCTGGAATGTAT TTTGTTAGTAAATGTATGTTTTCACCTTTTTTTAACATTTTAAATTATCAACATATAGTAGAGTTTATAT TCTCAATATGCCATATTTTTTAAGCAGCTTGCTTCTTACCTGTTTGTACACAGAGAGCCTAAGCCCAGTT TTGGAGGTTTTTTTTAATGTAGGGGCTGTGCCTTTGTTGTGTTGCAATGCTGGGGAGGAAGGTAAGAGTA CCATGCATGCTGTATGTAGAGGCTATGTCCTTACTGTGTGGAGCATTGTGTGCTTGGTATAACTGGCAGC CGTACATCCTTTACTGTGATATGATGGGCTAATGATTTTTATAGCACCTGGACTTAACTTTCTTTACAGA GACTTTTTCCCATAACTATTATTAGAACAAAGATATGCTTGAAAAATTATTAAATCAAGTTGCCTTCTGT TTTTTCCTAATATGTAAAACATTTTCCTAAATAAATGATAGAAAAGTAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3584640 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3584640 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_013775 same experiment
	EMAGE:31282 same embryo
	EMAGE:31280 same embryo
	EMAGE:30305 same embryo
	EMAGE:31284 same embryo
	EMAGE:31283 same embryo
	EMAGE:31277 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source