EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31318

Frem1 ( MGI:2670972)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_016475_01	euxassay_016475_02	euxassay_016475_03	euxassay_016475_04	euxassay_016475_05

euxassay_016475_06	euxassay_016475_07	euxassay_016475_08	euxassay_016475_09	euxassay_016475_10

euxassay_016475_11	euxassay_016475_12	euxassay_016475_13	euxassay_016475_14	euxassay_016475_15

euxassay_016475_16	euxassay_016475_17	euxassay_016475_18	euxassay_016475_19	euxassay_016475_20

euxassay_016475_21	euxassay_016475_22	euxassay_016475_23	euxassay_016475_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 08 09 10 11 13 15

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 09 weak expression: see section 10 11 13 14

telencephalon mantle layer

strong

regional

strong expression: see section 04 05 21 moderate expression: see section 20 weak expression: see section 03

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38838
Entity Detected:	Frem1, ( MGI:2670972)
Sequence:	sense strand is shown >T38838 CTTTTGAAAGGCCAGACTCACTCTTGGAAAGGATTTTGATGTGACTTTGAGCCTTTAAATCTGTAATATT TGCAGAGACACTCTTCTAATTAGATTCTCCAATAGAGGGTAGCTTATTAATTTATAAACTCAGTTGAGTG AGACATTTTTGTAGCATTCAAGCATGCTGTGCTTCTCCCAACAATCCCTTCTGGAGCCCAGGGTTAATTA TAGGAAGTCAGAACGTCCTTTTTGAAATGTTTATTAAAATTTTTCTTTTCATAATCTCTAAAAAATACAG CTGTGCCAAATATAACCTGTCATATGAGCACCCGTGTAACTTTAGAATTAAATGAGGTGTTCAAGTAGAC TAGAGCTTTAGGGCAGACTTTTTAAATAGAATAATAATATCTCGAGTGTTATAGACACTAAAAATGGCTT AAGAAAGTCGTTCCAGTTTCTGGATATAAAACATGACAGGCTTGCTTCATTTCTGCTCATTTATCAAAGT TTCGGTTCATAGAAATTTTTACCTAAGGTTAGGATAAGACTCAGACATTCTGTCTCTTGCTGAAATCCAG ATGTAATTGCAGAGCCGGGTGCTCTTAGCTTGGAGGTTTGGAGAAGTTTGTAGACCGTAAGTGCAAAGGC CTGTGGAACATAAGGAACCTTTAGTGTTTAACCCAAACAGGTCAAATCCTTCCTCCACTCGGGCTCCAGC CAAGCTCCTTGTAACCTA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 236792. Forward Primer - name:165947_F_cDNA_BC037594, sequence:CTTTTGAAAGGCCAGACTCACT; Reverse Primer - name:165947_N_SP6_cDNA_BC037594, sequence:TAGGTTACAAGGAGCTTGGCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_016475 same experiment
	EMAGE:31319 same embryo
	EMAGE:31322 same embryo
	EMAGE:30320 same embryo
	EMAGE:31320 same embryo
	EMAGE:30038 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source