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EMAGE:31324

1110001A07Rik ( MGI:1913390)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 17 18
thymus primordium
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15
telencephalon ventricular layer
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
midbrain ventricular layer
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16 17
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 16 17 weak expression: see section 15
right lung
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17 18 19 20 21 22
metanephros
moderate moderate
regionalmoderate expression: see section 06 07 08 09 15 16 17
left lung
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12
retina
moderate moderate
regionalmoderate expression: see section 01 02 03 04 21 22 23
diencephalon lateral wall ventricular layer
strong strong
regionalstrong expression: see section 13 14
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35333
Entity Detected:1110001A07Rik, ( MGI:1913390)
Sequence:sense strand is shown

>T35333
GACTAATCACCCACATTCAGCAGGAGAGAAAAATGCAGTTACAGTTTTAAAGGAATTATCAGCGATAAAG
TCTCGCTATCAAGCTTTATGTGCACGCTTTAAGGCAGTTTCTGTTGAGCAAAAGGAGACCAAGAGCTGCA
TTTGTGCTACTTTGAACAAGACAATGACCATGATACAAGAACTACAAAAGCAAACAAACCTGGAGCTAAC
TCTACTGACTGAAGAAGAGAAAGCTGCAACAGAGCCATTAAAATCTCATATGCCGGACTGATAAAAAATG
TTAAAGAGGAAGGCTGGTGGAAAGAAGACCAACTGCAGAGATTGGGCGAGACGTCTAGAGGCGGGGCTGA
TGAGGAAGCCTGACATTTCCCTTGACCGAGAGGATCGTGATGCATTTCAGATGAGGAAATTGTAAGAAGC
GTAGATGTCCTGTGACTGAAAACCAAAAGGATGGGATGGAAATAAAGCAATGTAAATACTGATACCCAGC
ATTCATTTTAGGCAATGAAATCCCTTAGTACTACTTTTTTTTTTATTTTCTGTTTTCTTTTTGTATAGTG
TTGGCATAATTTTAGATGTTTTGGGATTTTGTAAAATAATATTTATGTACATTTTGTTCAGTATGTAAAT
GGGCAAAATACTTGTATAAAAATTCAGGTGTTGTTTGCTCTTTTGAGACAAGGTTTCACTATGTAGCTCT
GGCTGGCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 64540. Forward Primer - name:064540_F_cDNA_1110001A07Rik, sequence:GACTAATCACCCACATTCAGCA; Reverse Primer - name:064540_N_SP6_cDNA_1110001A07Rik, sequence:GGCCAGCCAGAGCTACATAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_007512 same experiment
 EMAGE:30319 same embryo
 EMAGE:30050 same embryo
 EMAGE:31329 same embryo
 EMAGE:30326 same embryo
 EMAGE:31326 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS