EMAGE mouse gene expression spatial database

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EMAGE:31330

Syt1 synaptotagmin I ( MGI:99667)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003151_01	euxassay_003151_02	euxassay_003151_03	euxassay_003151_04	euxassay_003151_05

euxassay_003151_06	euxassay_003151_07	euxassay_003151_08	euxassay_003151_09	euxassay_003151_10

euxassay_003151_11	euxassay_003151_12	euxassay_003151_13	euxassay_003151_14	euxassay_003151_15

euxassay_003151_16	euxassay_003151_17	euxassay_003151_18	euxassay_003151_19	euxassay_003151_20

euxassay_003151_21	euxassay_003151_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

strong

regional

strong expression: see section 10 12

pituitary gland

moderate

regional

moderate expression: see section 10 11 12 13 14 15

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 17 18 19

cervico-thoracic ganglion

strong

regional

strong expression: see section 10 15 16

spinal cord

strong

homogeneous

strong expression: see section 09 10 11 12 13 14 15 16

thoracic ganglion

strong

regional

strong expression: see section 11 12

esophagus

moderate

regional

moderate expression: see section 11 12 13

facial vii ganglion

strong

regional

strong expression: see section 04 05 20

not examined

regional

not examined expression: see section 01 02 03 21 22

midbrain

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

vagus x ganglion

strong

regional

strong expression: see section 08 17

diencephalon

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 17 18 19 20 21

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 11 12 14 15 16 moderate expression: see section 07 08 09 10 17 18 19

hindgut

strong

regional

strong expression: see section 10 11 12 13

telencephalon marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

cervical ganglion

strong

regional

strong expression: see section 09 16

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

hindbrain

strong

homogeneous

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 20

neural retina

strong

regional

strong expression: see section 01 02 03 21 22

olfactory cortex marginal layer

strong

regional

strong expression: see section 10 11 12 14 15 16 17

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07

foot mesenchyme

moderate

regional

moderate expression: see section 02 03 04 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 18 19

midgut

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 15 16 17 18 moderate expression: see section 14

hand mesenchyme

moderate

regional

moderate expression: see section 02 03

limb

moderate

regional

moderate expression: see section 02

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5393
Entity Detected:	Syt1, synaptotagmin I ( MGI:99667)
Sequence:	sense strand is shown >T5393 GGGGACCAAGACTCGCACCATCTCCCGGTCGGTCCTCGCTCCAGGAAAAGAAGACTTTGGCGCGCTCGAA CAACCAACATCCGCAGTCTGATCAGAAGACATTGCCCTTGCCGTCCCGAGAGCTCCAGCAGAACATCTCG TTAAGATTGAAGAAAGGAGATTCCAAAAGGAACAAAAAAACCCAAATACTCCAGACTACCCCAGCAGAAC ATTCCGCTTGAACCAAAAATGGTGAGTGCCAGTCGTCCTGAGGCCCTGGCTGCCCCTGTCACCACTGTTG CGACCCTTGTCCCACACAACGCCACTGAGCCAGCCAGTCCTGGGGAAGGGAAGGAAGATGCCTTTTCCAA GCTGAAGCAGAAGTTTATGAATGAACTGCATAAAATCCCATTGCCACCGTGGGCCTTAATTGCCATAGCC ATAGTTGCGGTCCTTCTAGTCGTGACCTGCTGCTTCTGTGTCTGTAAGAAATGTTTGTTCAAAAAGAAAA ACAAGAAGAAGGGAAAGGAAAAGGGAGGGAAGAACGCCATTAACATGAAAGACGTGAAAGACTTAGGGAA GACCATGAAGGATCAGGCCCTTAAGGATGACGATGCTGAAACTGGACTGACTGATGGAGAAGAAAAGGAG GAGCCCAAGGAAGAGGAGAAACTGGGAAAGCTTCAATATTCACTGGACTATGACTTCCAGAATAACCAGC TGCTGGTGGGAATCATCCAGGCTGCTGAACTGCCCGCCCTGGACATGGGAGGCACATCTGATCCATACGT CAAAGTCTTCCTGCTGCCCGACAAAAAGAAGAAGTTTGAGACAAAAGTCCACCGGAAAACCCTCAATCCA GTCTTCAATGAACAGTTTACTTTCAAGGTGCCATACTCGGAATTAGGTGGCAAGACACTGGTGATGGCTG TGTATGATTTTGACCGCTTCTCCAAGCACGACATCATTGGAGAGTTCAAAGTTCCTATGAACACCGTGGA TTTTGGCCACGTCACCGAGGAGTGGCGCGATCTCCAGAGTGCTGAGAAAGAAGAGCAAGAGAAACTGGGT GACATCTGCTTCTCCCTCCGCTACGTCCCTACTGCCGGCAAGCTGACTGTTGTCATTCTGGAAGCCAAGA ACCTGAAGAAGATGGATGTGGGTGGCTTATCTGATCCCTATGTAAAGATTCACCTGATGCAGAACGGCAA GAGACTGAAGAAGAAAAAGACAACGATTAAGAAGAACACACTTAACCCCTACTACAATGAGTCCTTCAGC TTTGAAGTTCCGTTCGAGCAAATCCAGAAAGTGCAAGTGGTGGTAACTGTTTTGGACTATGACAAGATTG GCAAGAACGACGCCATCGGCAAAGTCTTTGTGGGCTACAACAGCACCGGCGCAGAGCTGCGACACTGGTC AGACATGCTGGCCAACCCCCGGCGACCCATCGCCCAGTGGCACACTCTGCAGGTAGAGGAGGAGGTTGAT GCCATGCTGGCTGTCAAGAAGTAAAGGGGAAAAGAAGCCTTTCTGCGTCTGCCCACATAGTGCTCTTTAG CCAGTATCTGTAAATACCTCAGTAATATGGGTCCTTTCAGTTTCCAGCCATGCATTCCTGATACAATCCA GTGGTACTTCAGATCCTGTTTTAATTTGCACAAATTTAAGTGTAGAAAGCCCCTATGCCCTTCATCATAC CACTGCCCTCCAAATCTACTCTTCTTTTAAGCAATATGATGTGTAGATAGAGCATGACTGAAATGTATTG TATCACACCGTTGTATATACCAGTATGCTAAAGATTTATTTCTAGTTTGTGTATTTGTATGTTGTAAGCG TTTCCTAATCTGTGTATATCTAGATGTTTTTAATAAGATGTTCTATTTTAAACTATGTAAATTGACTGAG ATATAGGAGAACTGATAATATATTATATGGTAAATATAGTATCGTCTGCATTCCAGCAAAAATATCAATT TGAAAGGCACTAGTACAGTTAAACCAACATCTTAAAGGACAACTTAAACCTGAACTTTCTATTGAATCCT TTGAGTACCAAGATTTGCTCACATGACATCTTTGATGGGTGAACCCAATTTTGTAGAATTCTTTCACAGG CAAATAGCATGTTCTGAGCAGCATCCATGCTGACCTCAAGGAAGCAAAGCCACAAACCAGAATAGCATCT GTCTGTCTGTCTCTGCAAAGCTAAAAAATCATGGCTTCACTCTTCCAGTCAAGGAAGCAACGAACACGAA GGAGCCAGTGAGTTCCTACGACTGCATCTAGCATAGCATAACCGTGGTGTTCTCTGTGTGTGCGTGTGCA AGTGTGAAAGTGTATGCACGAGTCTTATGTGTGGTGCATGCCTTTGTTCGGGGTTGGGGTGGGAGAGGAG AAGCTGAGAGAAGTCAGCGTTTCTGAAATGTTGCCTGACTACTTAAACAGAAACTAATAGCTCTCCAGTA TCACATTTATATAACACGTGCAACCTGGGAATTCTGGTCCAGATTTCACCCCAATATTGATTCCAAAAGG TATGCACGTGAGACTTTGTAACAAAATATTTTATTATATAAAACCAGATTAGAAGGAATGCAGAATATTT TTAACACAGCAATCTGTGCTTATTCCACAACATTACTTTGTGGTAAACAGACAGTATTGTAATCCCATGA AAAGATGAAATATAACAATTAGCCATAGTTCTGAATGCACTTCGATTAAGCCAAAACAGACAGCTAGTGA TCTTTTTATATGCTCTTTTTACTTAAGTTTTAATTTGTCCTTTAAACAAAGGTGAAAAAAAAAACCAAGA ACAAGTTCTAGCAAACTGAAGCAACCTCTTATGTACACTAGATGCTTGACTTAGGAGGAGTTTTTAAATG TTCTCAATGTTATTCTGTAGTAAATGGCACTATTATGAAGCCACTAGTCATTCCATATGAGTCTTAAGGA CTGCTCTGTGTAACACTGTGACTGCCCTGTGTGCTTAGACACGTAGTTTCCTCAGTGGCTAGCACCCAAC TTACTCCGTAGTGATATTGTAACAATACTGCCATTCCCTCGTACTGCACTGCCCAATGTGTGTGTAGCAC AAACAGTTCTCATTCTTAAGGACCAATTCAGAACTGAACAGCTATGCATAGGACAGAAAGATACATAGAC TGGGGGGGTGGGGAGAACACACAGCATCTTGTCAACACTGTGCACTAGTCCACATTTGTCTCGCTGAGGT AGACAGCCACTTCAGGAAGGTGAGCCTGCTACCTAACACTGCTTCTAGACTCTTCGCCCACTTGATAGTG CGGCCTGTTTTCACCTCCAGGTCACAGAAAACAGCAACATCCTGACGGGAGAGGACCATCTTCACATCTA CCAAAATAAAATGGAGGCATGGAAAGTGTGGCCTCCTGTTTGATCTATAGGATTTAGCTCCATGCTTTGG ACAAAAGTCAAGAGAATCCCAGAAAGGGCTCTTGGTAGACTTTAAACATGGGGTTGGCCGTTCCTCCTGC ACACACAACACAATCTCCAAACCCTGGTTCTTCCCTCATTGGAGAAGAGGCAAAGCCTTTGCTCCTCACC TCCATCCCAGCAAACTCTTCAGAATTAGAACAGATGCAGAGGACAAGGAGAAAATCCTTTCCTGTGGACG AACAGCACATTTTCAACATTAAAACCACAGATGATAGGAAAGGCACACTCATAGGTGAGATTAAACAGGA GACTCAAATGAGGGGCTTTCCTACTTAAGGGACCAAGTCCTACCAAAGAGAAGGAGTTGACTTTAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5365461 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5365461 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003151 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source