EMAGE mouse gene expression spatial database

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EMAGE:31333

Tubg2 tubulin, gamma 2 ( MGI:2144208)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003153_01	euxassay_003153_02	euxassay_003153_03	euxassay_003153_04	euxassay_003153_05

euxassay_003153_06	euxassay_003153_07	euxassay_003153_08	euxassay_003153_09	euxassay_003153_10

euxassay_003153_11	euxassay_003153_12	euxassay_003153_13	euxassay_003153_14	euxassay_003153_15

euxassay_003153_16	euxassay_003153_17	euxassay_003153_18	euxassay_003153_19	euxassay_003153_20

euxassay_003153_21	euxassay_003153_22	euxassay_003153_23	euxassay_003153_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 18 19 20

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 10 16

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19

thoracic ganglion

moderate

regional

moderate expression: see section 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 16 17 18 19 20

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 21 22

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 16 17 18 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 09 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 18 19 20 21 22 23

vibrissa

moderate

regional

moderate expression: see section 06 07 21 22 weak expression: see section 08 20

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 13 15 16 17 18 19 20

cervical ganglion

moderate

regional

moderate expression: see section 09 10 weak expression: see section 17

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4213
Entity Detected:	Tubg2, tubulin, gamma 2 ( MGI:2144208)
Sequence:	sense strand is shown >T4213 CAGAGAGAGGCTATGAGAGAAAATGATGCCCAGGATGGGCTCTGCCGCCCGCCGGCTGCGGAGAGGCCCA CACTGCACACGCGCAGACCCAGCGCCCGCGTCAAAACATCAGTCGACAGCGCGTTCCCCATGTCCTGCGC CTCCGGCTGGCAGGAGTTCCTCTCAGTCGTGACCCCCGCCGCCAGGCCGGGGCGGACGAGCTAGGTCTGA TCGGCGATGCCCCGGGAGATCATCACCCTGCAGCTGGGCCAGTGCGGCAACCAGATTGGGTTCGAGTTCT GGAAACAGCTGTGTGCTGAACATGGAATCAGCCCCGAGGGCATCGTAGAGGAGTTCGCCACCGAGGGCAC TGACCGAAAGGATGTCTTTTTCTACCAGGCAGATGATGAGCACTACATCCCCCGGGCTGTGCTGCTGGAC CTGGAGCCCCGGGTGATCCATTCCATCCTCAACTCCTCCTATGCTAAGCTCTACAACCCGGAGAACATCT ACCTGTCGGAGCATGGCGGAGGAGCTGGCAACAACTGGGGCAGAGGATTCTCCCAGGGTGAGAAAATTCA CGAGGACATCTTTGACATTATAGACCGAGAAGCAGATGGAAGTGACAGTCTAGAGGGATTTGTGCTATGT CACTCCATTGCTGGGGGGACAGGTTCTGGTCTGGGCTCCTACCTCTTAGAGCGACTGAACGACAGGTACC CCAAGAAATTGGTGCAGACATACTCAGTGTTTCCCAACCAGGACGAGATGAGTGATGTAGTGGTCCAGCC CTACAACTCCCTCCTCACACTAAAGAGGCTGACCCAGAATGCGGACTGCGTGGTGGTGCTGGACAACACA GCCCTGAACCGGATCGCCACAGACCGCCTACACATCCAGAACCCATCCTTCTCCCAGATCAACCAGCTGG TGTCCACCATCATGTCAGCCAGCACCACCACCCTGCGCTACCCTGGATACATGAACAATGACCTCATCGG CCTCATCGCCTCGCTCATTCCCACCCCTCGGCTCCACTTCCTCATGACTGGCTACACCCCCCTCACCACG GACCAGTCAGTGGCCAGTGTGAGGAAGACAACAGTCCTGGATGTCATGAGGCGCCTGCTACAGCCCAAGA ATGTGATGGTGTCCACAGGTCGGGATCGTCAGACCAACCACTGCTACATCGCCATCCTCAACATCATCCA GGGAGAGGTAGACCCCACCCAGGTCCACAAGAGCCTGCAGAGGATCCGGGAAAGGAAACTGGCCAACTTC ATTCCCTGGGGCCCAGCCAGCATCCAGGTGGCCCTGTCAAGGAAGTCTCCCTACCTGCCCTCAGCCCACC GGGTCAGCGGGCTCATGATGGCCAACCACACCAGTATCTCCTCGCTTTTTGAAAGTTCCTGCCAGCAGTA TGACAAACTGTGGAAGCGGGGGGCCTTCCTGGAACAGTTTCGCAAGGAGGACATCTTCAAGGACAACTTT GAGGAGATGCACAGATCGAGGGAGGTGGTGCAGGAACTCATTGACGAGTACCACGCGGCCACCCGGCCGG ACTACATCTCCTGGGGTACCCAGGAGCAGTGACTCTACTCTCTGCTGTCCCCTGGACAAGAAGCACAGTC CCCACCACGCCCAGACCCTCTGACCCAACTTCGCCTCACATTTTTGTGTTCATGTGCAATTTAGGAACTG TTCCTGTCTTCCTTTTCACCCCTGGCGCTTGGCTGGCTTGGTTAAGTTCTAATAAATAAAGCGTGATGAT GCATTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4501003 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4501003 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003153 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source