EMAGE mouse gene expression spatial database

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EMAGE:31334

Cend1 cell cycle exit and neuronal differentiation 1 ( MGI:1929898)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003154_01	euxassay_003154_02	euxassay_003154_03	euxassay_003154_04	euxassay_003154_05

euxassay_003154_06	euxassay_003154_07	euxassay_003154_08	euxassay_003154_09	euxassay_003154_10

euxassay_003154_11	euxassay_003154_12	euxassay_003154_13	euxassay_003154_14	euxassay_003154_15

euxassay_003154_16	euxassay_003154_17	euxassay_003154_18	euxassay_003154_19	euxassay_003154_20

euxassay_003154_21	euxassay_003154_22	euxassay_003154_23	euxassay_003154_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

strong

regional

strong expression: see section 02 03 04 05

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 08 18 19

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 16 weak expression: see section 09 10

forebrain

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 weak expression: see section 15 16 17 18 19 20 21 22 23 24

spinal cord

moderate

regional

moderate expression: see section 07 08 09 10 11 12 weak expression: see section 16

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 18 19

thoracic ganglion

moderate

regional

moderate expression: see section 13 14 weak expression: see section 12

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 15 16 17 18

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 21 22

not examined

regional

not examined expression: see section 02 03 04 05 24

midbrain

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 weak expression: see section 15 16 17 18 19

vagus x ganglion

strong

regional

strong expression: see section 08

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 18 19 20 21 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 13 14 15 18 19 20 weak expression: see section 10 11 12 16 17 21

cervical ganglion

moderate

regional

moderate expression: see section 17 weak expression: see section 08 09

hindbrain

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 weak expression: see section 15 16 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5408
Entity Detected:	Cend1, cell cycle exit and neuronal differentiation 1 ( MGI:1929898)
Sequence:	sense strand is shown >T5408 GAAGCGAATGCGCAGCGAGAAGCTGGAGGGCCCAAAGAAGACACCCATATAATGCCCAGAGGTGAGCTGT CCTGAGCCACTGTGGTCTAGCCACCTGCCAGTCACACAAGCCCCAAACCAGCTGCCAGCAAAAAACCATC ATGGAATCCCGAGGAAAGTCAGCCAGCAGCCCCAAGCCAGATACCAAGGTGCCTCAGGCCACAGCTGAGG CCAAGGCCACTCCAGCTGCGGATGGGAAAGCTCCCTTGACCAAGCCTGTGAAGAAGGACACCCAAGCAGA GAAGCAGGAGCAAGCAGCAGCCCCTGGGCCAGCCGCTACGAAGAAGACACCAGCCAAGGCAGATCCTGTA CTTCTCAACAACCACAGCAACCTGAAGCCAGCTCCCACAGTGCCGGCAGCCCCCAGCAGTCCTGATGCAA CCTCAGAGCCCAAGGGTCCTGGGGATGGGGCAGAGGAGGACGAGTCCAACACTGGAGGCCGAGGTCCCTG GCCTTGTGAGAACTTGACCCCTTTGCTGGTAGCTGGGGGTGTGGCCGTGGCCACCATAGCTCTAATTCTT GGTGTGGCCTTTCTAGCCCGGAAAAAATGATACCTGGTGTCCAGGTCGGGGCACAGAGCCACTTTCTGTA CAGACCTGAATATATAGTGCATGCAAGGTCCTAGATATTTATCTATACATATACACCTAACAACACACAC ACAACACACACACACACTCACACGTATATGTATGCTACATACATCCGTGCAGCAGGAAAGAAATAGACAG TGAGCCCACCTCCACTCACCCCAGCACACCCTCTCCCAAGTTCTGGGGCAGGAGCCCAGCTCTGCAGTTT GCAGGAAGCACTAGCGTGGGGCAAAGGGGAGAGATGCTGGGTGTGGTCTCTGAAAGATAAGTGATGGAGT GCGCTAGCCCTGTGGGGACATTAAAGGGCATGGCTGTCTTACTCCTCTCACTTGTCTTTTGAGCAGCCAG GAGCAGTCTGTCTGGGCCTGATGCCGGGGGACTGACCTAAGGGTGGGTGGGATTCAAATCTGCAGAGCTA CCTCTACCCATTGTCCCTGTCCTGGTTGGATGGAAAAGGCAAAGTTAACAGCTGAGTTTGGGATGGGGTT CCCACCTCTTCCCTCTGTCAGTGTATGCCCCAGGAGAGCACCGGAGGCTAGGACCACCTTACTTCCTGCC CAGAGCAGCCAATTGTTCCGGGGAGGGGGTGGTTCTGAAAGCCTGGCAGGGAGGCAGGAAGCACGAAGGA GCCACACTACAGTTCTGCCTGCCCAATATTCCCCAATACTGTCCTTCCTGCCTGCACCCAACACCCCCCC CCTTGAGTTTAACAGCGTATAAATGCAATAACACAGGTAGAGTAGAACTGCCCGGTGAGTGGCAATCGCT CACTGCCTGCAGGAAGACCTGCTGTGCAGCCCCTCTTTAGTGCATCTGGCCCCAGCTTGCCCCTTGGTGT GGTGACAGTGAGCAGCTTTCCGGAAAACTGCAGTGGGCTCTGAGGAAACATTGCCCTCACCTGGCCAGTG GCGGCGGGGACCTTGGGCCGGAGTGGAGACTGAGTTTGAAATAAACCGTTACAATATAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5366891 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5366891 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003154 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source