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EMAGE:31342

Wee1 ( MGI:103075)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
weak weak
regionalweak expression: see section 06 07 09 17
telencephalon ventricular layer
moderate moderate
homogeneousmoderate expression: see section 09 10 11 weak expression: see section 01 02 03 04 05 06 07 08 14 15 16 17 18 19 20 21 22
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 09 10 11 weak expression: see section 12 13 14
hindgut
weak weak
regionalweak expression: see section 11 12 16
hypothalamus ventricular layer
weak weak
regionalweak expression: see section 11 12
olfactory cortex ventricular layer
moderate moderate
homogeneousmoderate expression: see section 10 11 weak expression: see section 15 16
stomach
weak weak
regionalweak expression: see section 03 04 05 06
lung
weak weak
regionalweak expression: see section 04 05 06 07 08 09 10 12 13 14 15
midgut
weak weak
regionalweak expression: see section 11 12 13
diencephalon lateral wall ventricular layer
weak weak
regionalweak expression: see section 11 12
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63154
Entity Detected:Wee1, ( MGI:103075)
Sequence:sense strand is shown

>T63154
AATGCTGTCTCTGAGGAAGGAGATGAAGATGACTGGATATCCAATAAAGTTATGTTTAAAATAGGTGATC
TTGGGCATGTAACAAGAATCTCTAGTCCTCAAGTTGAAGAAGGTGATAGTCGTTTTCTAGCAAATGAAGT
TTTACAAGAGAACTATAGCCATCTACCGAAAGCAGATATTTTTGCTCTTGCTCTCACAGTCGTATGTGCT
GCTGGTGCTGAACCCCTTCCCAGAAATGGAGAGCAGTGGCACGAGATCCGGCAGGGTCGGTTACCTCGGA
TCCCACAAGTGCTTTCCCAGGAGGTGACAGAGTTGCTGAGAGTCATGATTCATCCTGACCCAGAGAGAAG
GCCTTCAGCAATGGAGCTGGTGAAGCATTCAGTGTTGTTGTCTGCATCTAGAAAGAGCGCAGAGCAGTTA
CGAATAGAATTGAATGCTGAAAAATTCAAAAATTCTCTTTTGCAGAAAGAACTCAAGAAAGCCCAAATGG
CAGCCAAAGTTGCAGCTGAGGAACGGGCACTCTTCACAGATCGGATGGCCACTAGATCCACCACCCAGAG
TAATAGAACTTCTCGACTTATTGGAAAGAAAATGAACCGCTCTGTCAGCCTTACTATATACTGAACTGCT
CACATTCCCCAGCCCTTCCCCACTCAAAGGAAAAAACTGTGACAAGAGGAAGCTAGGTTGGAATCACTGC
TAGAGCCCACTTCGTGATTACTTGCCCATTGGTATCAGTAGTTTTCCTAAAACGTAACGCCTTCTGTGAC
TTTGAGAATTACAGTTGAGAGCTGTATTCTGACTTGGCTCTGTCAGGCTTTTGTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 143040. Forward Primer - name:143040_F_cDNA_Wee1, sequence:AATGCTGTCTCTGAGGAAGGAG; Reverse Primer - name:143040_N_SP6_cDNA_Wee1, sequence:GACAAAAGCCTGACAGAGCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_016462 same experiment
 EMAGE:30365 same embryo
 EMAGE:30348 same embryo
 EMAGE:30337 same embryo
 EMAGE:30357 same embryo
 EMAGE:30356 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS