EMAGE mouse gene expression spatial database

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EMAGE:31349

Slc5a1 solute carrier family 5 (sodium/glucose cotransporter), member 1 ( MGI:107678)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_019738_01	euxassay_019738_02	euxassay_019738_03	euxassay_019738_04	euxassay_019738_05

euxassay_019738_06	euxassay_019738_07	euxassay_019738_08	euxassay_019738_09	euxassay_019738_10

euxassay_019738_11	euxassay_019738_12	euxassay_019738_13	euxassay_019738_14	euxassay_019738_15

euxassay_019738_16	euxassay_019738_17	euxassay_019738_18	euxassay_019738_19	euxassay_019738_20

euxassay_019738_21	euxassay_019738_22	euxassay_019738_23	euxassay_019738_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

hindgut

moderate

regional

moderate expression: see section 17

metanephros

strong

regional

strong expression: see section 10 11 22 23 24 moderate expression: see section 12 weak expression: see section 13

midgut

strong

regional

strong expression: see section 09 10 11 moderate expression: see section 03 04 05 06 07 08 12 13 17 18 19 20 21 22 23 weak expression: see section 14 15 16 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T56061
Entity Detected:	Slc5a1, solute carrier family 5 (sodium/glucose cotransporter), member 1 ( MGI:107678)
Sequence:	sense strand is shown >T56061 CGGACGCGTGGGGCTCGGTCTTCTTAAGGCACTGCACACCTAGGAGCTGCTTCCCGACGGTGCTGTGCAG CGCAGCAGCACAGGGGCCCAGCGTTCCTGCCGCGCAGCCCCACTGTCGCGGGTCCCCTCCCTCGGGGCTG ATCATTAACTCAAAAGCAGTATAAGGAGTGAGTGGCCCCGGTGAGCCTGTGAGCCGAGAGGGACTCGTCG CCACCGCCATGGACAGTAGCACCTTGAGCCCCGCGGTTACTGCCACCGATGCACCCATCCCGTCCTACGA GCGCATTCGCAATGCGGCTGACATCTCAGTCATCGTCATCTACTTCGTGGTGGTGATGGCTGTCGGGCTG TGGGCCATGTTTTCCACTAATCGTGGTACCGTTGGAGGCTTCTTCCTGGCAGGCCGAAGTATGGTGTGGT GGCCGATTGGAGCCTCTCTCTTTGCCAGTAACATTGGAAGTGGTCACTTTGTGGGGCTGGCAGGCACAGG AGCAGCCGCAGGCATTGCCATGGGTGGCTTTGAATGGAACGCCTTGGTTTTGGTGGTTGTGCTGGGCTGG ATATTTGTCCCGATTTACATCAAGGCCGGGGTGGTGACGATGCCGGAGTATCTGCGGAAGCGGTTTGGAG GCAAGAGAATTCAGATCTACCTTTCCGTTCTGTCCCTGCTCCTCTACATCTTCACAAAGATCTCTGCAGA CATCTTCTCCGGGGCTATATTCATCAATCTAGCCTTGGGCCTGGATATATACTTGGCCATTTTCATCCTG CTAGCAATCACTGCCCTTTATACAATCACAGGGGGGCTGGCAGCTGTAATTTACACAGATACCCTGCAGA CCGCGATCATGCTGGTGGGGTCTTTTATCCTGACTGGGTTTGCTTTCAATGAAGTAGGAGGATATGAAGC CTTCATGGACAAGTACATGAAAGCTATTCCAACTAAGGTTTCTAACGGAAATTTTACCGCCAAGGAAGAA TGCTACACACCGAGGGCTGACTCATTCCACATATTCAGAGACCCCATTACTGGAGACATGCCGTGGCCTG GGCTCATTTTTGGCCTGGCCATCCTCGCCCTGTGGTACTGGTGTACGGATCAGGTCATTGTGCAGCGATG CCTCTCGGCCAAGAACATGTCTCACGTGAAGGCTGGCTGTACCCTGTGTGGCTATCTGAAGCTACTGCCC ATGTTCCTCATGGTGATGCCAGGAATGATCAGCCGGATTCTATATACAGAAAAAATCGCCTGTGTCCTCC CTGAAGAATGTCAGAAATACTGCGGCACACCAGTGGGCTGTACCAACATCGCCTACCCCACGCTCGTGGT GGAACTCATGCCTAATGGACTGCGAGGCCTGATGCTGTCTGTCATGATGGCTTCCCTCATGAGCTCCTTG ACCTCCATCTTCAACAGCGCCAGTACTCTCTTCACCATGGACATCTACACCAAGATCCGGAAGAAGGCAT CTGAGAAGGAGCTCATGATTGCAGGAAGGTTGTTCATCCTCGTGCTGATTGGCATCAGCATTGCCTGGGT ACCCATTGTACAGTCTGCTCAAAGTGGGCAGCTTTTTGATTACATCCAGTCTATCACCAGTTACCTGGGG CCGCCCATTGCAGCTGTCTTCCTGCTTGCCATTTTCTGCAAGAGAGTCAATGAACAAGGAGCCTTCTGGG GACTGATCCTGGGCTTCCTGATTGGAATCTCCCGTATGATCACTGAGTTTGCCTATGGAACTGGGAGCTG CATGGAGCCCAGCAACTGCCCCAAGATCATCTGTGGGGTCCACTATTTGTACTTTGCCATCATCCTCTTC GTCATCAGCGTCATCACCATCTTGATCATCTCCTTCCTCACCAAGCCCATTCCAGACGTGCACCTGTACC GTTTGTGTTGGAGTCTACGCAACAGCAAGGAAGAGCGAATCGACCTGGATGCAGGAGAGGAGGAGGACAT CCCAGAGGACTCCAAGGACACAATTGAAATAGACACAGAAGCCCCCCAGAAGAAGAAAGGATGCTTCAGG AGAGCATATGACCTGTTCTGTGGGCTGGACCAAGATAAAGGACCAAAGATGACTAAGGAGGAGGAGGAAG CCATGAAGATGAAGATGACAGACACTTCTGAGAAGCCTTTGTGGAGGACAGTGGTGAACATCAACGGTAT TATCCTGCTGGCGGTAGCTGTCTTCTGCCATGCCTATTTTGCCTGAGCCCTGCCTTCTGGTGTAGACTCG GCAACTCTAGCATTGTTCTTCCCCCTTTTGACCCACTCTGTAGTGGCAAGGGGAAGATGTCCACTTGTAA ATTTCACGCATTCGGATAAAAGGTGTATAAGTGTGATTATAGGCTTGCTGGAGGAAAGTGATTACATTGC TGTGAACTTATGCATTTGAAGCCAGTGTGCCACACTCATCTGCCATGCCAGAGCTTTAGAAGTGAAGTTG ACTCAGATATAGGAAAAGTCATGCCAAGACACAGAAGCCCTGTGTTGTCTGATGTCACTCTGTCTCAGCT TGTAATTATTGTTTATAATCCTTAAATACTATTTTTTAAAATTGTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. .
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_019738 same experiment
	EMAGE:13375 same embryo
	EMAGE:13376 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source