EMAGE mouse gene expression spatial database

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EMAGE:31352

Tfip11 ( MGI:1930075)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005104_01	euxassay_005104_02	euxassay_005104_03	euxassay_005104_04	euxassay_005104_05

euxassay_005104_06	euxassay_005104_07	euxassay_005104_08	euxassay_005104_09	euxassay_005104_10

euxassay_005104_11	euxassay_005104_12	euxassay_005104_13	euxassay_005104_14	euxassay_005104_15

euxassay_005104_16	euxassay_005104_17	euxassay_005104_18	euxassay_005104_19	euxassay_005104_20

euxassay_005104_21	euxassay_005104_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 15 16 17 18

metanephros

moderate

regional

moderate expression: see section 04 05 06 07 08 13 14 15 16 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 05 11

spinal cord

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

thoracic ganglion

moderate

regional

moderate expression: see section 10 11

retina

moderate

regional

moderate expression: see section 01 02 03 04 21 22

thyroid gland

moderate

regional

moderate expression: see section 07 10 11

dorsal root ganglion

moderate

regional

moderate expression: see section 04 06 07 08 09 10 11 12 weak expression: see section 03 05

lower jaw molar

moderate

regional

moderate expression: see section 06 07 17 18

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 15 16

upper jaw molar

moderate

regional

moderate expression: see section 06 07 17 18

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

thymus primordium

moderate

regional

moderate expression: see section 07 08 09 10 11

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 15 16 17 18 19 20

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 13 16

vibrissa

moderate

regional

moderate expression: see section 06 07 08 09 20 21

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19

cervical ganglion

moderate

regional

moderate expression: see section 05 13

naris

moderate

regional

moderate expression: see section 13 17

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 14 15

upper jaw incisor

moderate

regional

moderate expression: see section 11 15 weak expression: see section 12

nasal septum

moderate

regional

moderate expression: see section 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7525
Entity Detected:	Tfip11, ( MGI:1930075)
Sequence:	sense strand is shown >T7525 CTGGGCGAAGGCTGTGTGGCTCTCGCGGATGCTGAGCGAGGTGTGACTGCAAAGTGGCATTCAGAAAGAC TGCAACCTTCCAGTACCGTAGAAGCCGTTCAGGGAAGGAAACCTTCAGTCCCGTGGAAATAGTATTTCAG AAAATGCTCTGAAGTTTCCCTGGAACTGCAAATTGGCTAGATACAACTTTTCCTGGAGAAGTAAAAAGAC CAACCTGGTTTCTGAGTGACTGGCCATGTCTCTGTCCCACTTGTACCGGGATGGCGAAGGCCACCTTGAT GATGACGATGACGATGAGAGGGAGAACTTTGAGATCACCGACTGGGATCTCCAGAATGAGTTCAACCCCA ACCGGCAGCGCCATTGGCAGACCAAAGAGGAGGCCACCTACGGGGTGTGGGCGGAGCGCGACTCGGACGA GGAGAGGCCCAGCTTTGGAGGCAAAAGGGCCCGAGACTATTCTGCACCTGTCAACTTCATCAGTGCGGGG CTCAAGAAAGGGGCGGCTGAGGAAGCGGACTCCGAGGACTCTGATGCTGAAGAGAAGCCTGTTAAGCAGG AGGACTTCCCGAAGGATTTAGGACCAAAGAAGTTAAAGACGGGTGGCAATTTTAAGCCCAGCCAGAAAGG CTTTTCAGGAGGAACCAAGTCCTTCATGGACTTTGGCAGCTGGGAGAGACACACGAAAGGGATCGGGCAG AAGCTGCTGCAGAAGATGGGCTACGTCCCTGGGCGTGGCCTGGGGAAGAACGCACAGGGGATCATCAACC CCATCGAAGCCAAACAGAGAAAAGGCAAGGGAGCCGTGGGGGCCTATGGCTCGGAGAGGACCACTCAGTC TCTGCAGGACTTCCCCGTGGCCGACTCGGAAGAGGAGGCAGAAGAGGAGTTTCAGAAGGAGCTGAGCCAA TGGAGGAAAGACCCCAGCGGGAGCAAGAAGAAGCCAAAGTACTCTTACAAGACTGTGGAGGAGCTGAAGG CCAAGGGCAGGGTCAGCAAGAAGCTCACAGCACCTCAGAAGGAACTGTCTCAGGTCAAGGTGATCGACAT GACAGGCCGGGAGCAGAAGGTGTACTACAGCTACAGCCAAATCAGCCACAAGCACAGCGTGCCCGATGAA GGGGTGCCATTGCTGGCGCAGCTGCCCCCCACAGCCGGCAAGGAAGCCAGGATGCCGGGCTTTGCACTGC CTGAGCTGGAGCACAACCTGCAGCTGCTCATTGAGCGCACGGAGCAGGAGATCATCCAGAGCGACCGGCA GCTCCAGTATGAGCGGGACATGGTGGTCAGCCTGTCGCATGAGCTGGAGAAGACGGCCGAGGTTCTTGCA CATGAGGAGCGTGTCATCTCTAACCTCAGCAAGGTGCTGGCCCTGGTGGAGGAATGTGAGCGCCGCATGC AGCCCCATGGCACCGACCCCCTCACTCTGGATGAGTGTGCCCGCATCTTTGAGACACTACAGGACAAGTA TTATGAGGAGTACCGCCTGGCGGACCGCGCAGACCTCGCTGTGGCCATTGTCTACCCGCTCGTGAAGGAC TACTTTAAGGATTGGCACCCCCTCGAGGACGGCAGCTATGGCACCCAGATCATCTCCAAGTGGAAGAGCC TCCTGGAGAACGACCAGCTGCTGTCTCACAGCAGCCAGGACCTGTCCTCCGATGCCTTCCACAGGCTCAT GTGGGAGGTCTGGATGCCTTTCGTTCGGAATGTCGTCGCCCAGTGGCAGCCACGGAACTGTGAGCCGATG GTGGATTTCCTGGACAGCTGGGCACACATCATCCCCGTGTGGATCCTGGACAATATCCTGGACCAGCTCA TCTTCCCTAAGCTGCAGAAGGAGGTGGACAACTGGAACCCCCTGACAGACACCGTTCCCATCCACTCGTG GATCCACCCGTGGCTGCCGCTCATGCAGGCCCGCCTGGAGCCACTCTACTCCCCTGTGCGCAGCAAGTTG TCCAGCGCACTGCAGAAATGGCACCCCAGCGACGCCTCAGCCAAGCTCATCCTGCAGCCCTGGAAAGAGG TCCTCACCCCTGGGTCCTGGGAGGCCTTCATGCTCAGGAACATCGTGCCCAAGCTGGGCATGTGCCTGGG AGAGCTTGTCATCAACCCCCACCAGCAGCACATGGACGCCTTCTACTGGGTGATGGACTGGGAGGGGATG ATCTCCGTCTCCAGCCTGGTGGGGCTGCTGGAGAAGCATTTCTTCCCCAAGTGGCTCCAGGTGCTGTGCT CCTGGCTCAGTAACAGTCCCAATTACGAGGAGATCACCAAGTGGTACCTGGGCTGGAAGTCCATGTTCTC AGACCAGGTGCTGGCCCACCCTTCTGTCAAGGACAAGTTCAACGAAGCGCTCGACATCATGAACAGGGCT GTGTCCTCCAATGTTGGTGCCTACATGCAGCCGGGTGCGAGAGAGAACATTGCCTACCTCACCCACACAG AGCGCAGGAAGGACTTCCAGTACGAGGCCATGCAGGAGCGCCGGGAGGCTGAGAACATGGCACAGAGAGG CATCGGTGTGGCCGCCAGCTCCGTGCCCATGAACTTTAAGGACCTCATCGAGACTAAGGCAGAGGAGCAC AACATTGTGTTCATGCCTGTCATCGGCAAGCGGCACGAGGGCAAGCAGCTCTACACCTTCGGCCGCATCG TCATCTACATTGACCGGGGTGTGGTGTTTGTGCAGGGCGAGAAGACGTGGGTGCCCACCTCCCTGCAGAG CCTCATCGACATGGCCAAGTAGACATCGGCCACACCAGACCGGTGTGAATAAACAGCACTGTAAACCAAG CTGATGAATAACGTCTCTATTTGGGAGACCAGGCCACCAGGGCAGGATGTGACTCAGTGTCCCCAGTCGG CCTGTATTCCTGGCCGGGCAAAGGATCTGGTGTGGTGTGCACCGTGGCTCCTGGCCCAGGTGGAGAGAAC TGTCCTGTTACTCCAGCTGAGCGATCTGTAGCTTTCCTGATGATCTCCAGGTCCTCACAGTCCTGGTAGT CAGGCTCTTCCTGGGATGCCCACAGGTCCCCAGGCAGTCCTTCCAGCTTCAATAGTGGGAACCAGTGGAC AGATGTATCATTAAAGGTGTCAGTGTACTGGGGAGTCTGCGGCAGTGGGACCTCCTCCAGGCCTTTCAGA ATCTGAGCAGAGAGGACCAGACGGGCATTAGGTACACCCCTCTCTATCTTCACCTGGCCACCCCACCGTC CTTCTGAGAAATAAGGGATGGGGGGGTTGTATTTCAGCACTTTTCAGTCCCATGCCAGTGAGACTGCTGG GCTCGCCCTAAGGATGAAGGCCACTGGCACACAGTAGGAGAGGCACCAGCTGGTTGTGCTTAGAAACATG GACAGTGTGGCTTTGCACCCTCACCCCGGCCCAGCTATTCTGTCAGACACCTTTGCAATGTAGGGGTAAT TTTCCTGCAGAATCCTCGCCAACAACCTACAAATAAAAACATACATACACAATTAGGAAGTAAAAAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4239713 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4239713 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005104 same experiment
	EMAGE:31782 same embryo
	EMAGE:30808 same embryo
	EMAGE:30612 same embryo
	EMAGE:30567 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source