EMAGE mouse gene expression spatial database

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EMAGE:31358

Map4k1 mitogen-activated protein kinase kinase kinase kinase 1 ( MGI:1346882)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_004252_11	euxassay_004252_12	euxassay_004252_13	euxassay_004252_14	euxassay_004252_15

euxassay_004252_16	euxassay_004252_17	euxassay_004252_18	euxassay_004252_19	euxassay_004252_20

euxassay_004252_21	euxassay_004252_22	euxassay_004252_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 10 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6119
Entity Detected:	Map4k1, mitogen-activated protein kinase kinase kinase kinase 1 ( MGI:1346882)
Sequence:	sense strand is shown >T6119 CCCACGCGTCCGGTGGAGACAGAAGGTAATACCAAAGCTTGAACCCTGAGGCCCTGTTGGCCCCCTGGGC ACTGGCCCAGCCCCGGCCTCCAGGAATGGCCCTTGTGGACCCCGACATTTTTAATAAGGACCCTCGAGAA CACTATGATCTACTGCAACGACTGGGTGGTGGAACTTATGGAGAAGTATTCAAGGCTCGCGACAAGGTGT CTAAGGACCTGGTGGCTTTGAAGATGGTGAAGATGGAGCCTGATGATGACGTCGCCACCCTTCAGAAGGA GATTCTCATGCTGAAAACTTGCCGGCACGCCAACATCGTGGCTTACCATGGCAGTTACCTCTGGCTGCAG AAGCTGTGGATCTGCATGGAATTCTGTGGAGCCGGTTCTCTCCAGGACATCTACCAAGTGACTGGCTCTC TGTCCGAGCTCCAGATCAGCTATGTGTGCCGGGAAGTGCTTCAGGGACTGGCCTACCTGCATTCAGAGAA GAAGATACATCGAGACATCAAGGGAGCCAACATCCTCATCAACGACTGCGGGGAGGTTAAGCTGGCTGAC TTTGGGATCTCAGCCCAGATTGGGGCGACACTGGCCCGGCGCCTTTCTTTCATTGGGACACCGTACTGGA TGGCTCCAGAGGTGGCAGCGGTGGCCCTGAAGGGTGGATACAATGAGCTGTGCGACATCTGGTCTCTTGG CATCACAGCCATCGAGCTGGCTGAGCTTCAGCCCCCTCTCTTTGATGTGCACCCTCTCAGGGTTCTCTTC CTCATGACCAAGAGCGGGTACCAGCCTCCTCGGCTGAAGGAAAAGAGCAGATGGTCATCTTCCTTCCACA ACTTTGTCAAAGTCACCCTCACTAAGAACTCCAAGAAACGCCCCAGTGCCACCAAGATGCTCAGTCATCA GCTGGTGTCCCAGCCAGGGCTGAACAGAGGCCTGATCTTGGATCTTCTTGACAAAATGAAGAATCCTGGG AAGGGGCTGCCTGTTGATATTGAAGATGAGGAGCCTGAGCCACCCCCTGCCATCCCTCGGCGGATCAGAT CTACCTACCGGGCCAGCTCACTAGGGATCCCAGACGCAGACTGCTGCCGGCGGCAAATGGAGTTCCAGAG GCCCAGATGCGTGGACTGCAGACCTCAGGCTGAAACTGTTCGCTTATATCCCCCTGCACACTTCGGGAGC ACCAGTCCCAGGAGTCAGCTGTCAGATTCTGATGACGACTATGATGATGTGGACATCCCTGCCCCTTCAG AGAACATACCTCCTCCACTGCCCCCCAAGCCCAAGTTTCGGTCTCCATCAGATGATGGTTCTGGAGGAAT TAGGGATGATGGGCAATTGAGTCCAGGGGTTCTGGTTCGATGTGCAAGTGGGCCCCCTCCACGTACTCCC CGACCTGGACCTCCCCCAGCGACCCGCAGCCCTCACCTCACAGCTCGCTCAGATCCCTCATTGTGGAACC CAGCTGCTCCGGAGCCAGGCCAGCCCCCACTCGTACCCCCAAGGAAGGAAAAGATGAGGGGAAAGATGGA GAACGAGAAGCGCAGAGAGAAATATCCCCTTCTTGTGAAATTATTCAACGGCTGTCCCCTCCAGATCCAC AGCACAGCTGCCTGGACACATCCCTCTACAAAAGATCAGAACCTGCTCCTGGGGGCAGAGGAAGGCATTT TCATACTGAACCGGAATGATCAGGAGGCCACGCTGGAAATGATCTTCCCTGGCCGCACAACCTGGCTCTA TTGCATCAACAACCTCCTCATGTCTCTCTCAGGGAAGACACCCTACCTGTATTCTCATAGCATCCTGGGC CTGCTTGAAAGGAAAGACGGCAGAACAGGAAGCCCCATTGCTCACATTAGCCCTCACCGGTTACTGGCAA GAAAGAACATGGTCTCCAGTAAGATCCAGGACACCAAAGGCTGCCGGGCTTGCTGTGTGGGTGAGAGCCA GCTTCCCTCCGGGTGCAGAGTGATGGGGCGGGCCGGTGCCTGGCCAGTGTCTACTCAATAAGCTGAGAGT GCTAGCTCTGGGGGCCCATTCTTATGTGGGGCATTGGAGACGTCTGTGGTCTTGCTCCAGTGGTACCAAC CAATGAACAAATTCTTGCTTGTCCGGCAGGTGCTGTTCCCGCTGCCCACGCCGCTGCCGGTGTTCACGCT GCTGACAACGCCGGGCTCCGAGCTGCCGGCTGTGTGCATCGGCGTGAGCCCCGGGCAGGCGGCAAAGTCG GTGCTCTTCCACACCGTGCGCTTCGGTGCGCTGTCCTGCTGGCTGGACGACTCCAGCACGGAGCACAAGG GACCAGTGCAGGTGATCCAAGTAAAGGAAGACATGGTGATGGTGTTGATGGATGGCTCTCTAAAGCTAGT GACCCCAGAGGGGGCACCAGCTCCTGGGCTCCGCACCCCTGAGATCCCCATGACAGAAGCGGTCGAGGCT GTGGCTATGGTTGAAGATCGACTCGAGGCCTTCTGGAAGCATGGAGTTCAGGTGTGGGCTCCAGGCTTGA AACAGCCGCTGCAGGAGCTGAGAGACCCCACCCTCACCTTCCGTCTGCTCTGCTCCCCCAGGCCGGTAGT GGTGGAGACAAGGCCCACGGATGACCCTACGGCCCCCAGCAACCTCTACATCCAGGAATGAGCCATTGAG AGGGCATGGGAAACGGATGCCTGCAGACTCCTAACAGACGCACTAGTGGTCATGACATGACCTTATCTCC CAATAAACTTGACTTTAGTCTTGTCATCTTGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3494470 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3494470 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004252 same experiment
	EMAGE:29470 same embryo
	EMAGE:29472 same embryo
	EMAGE:29473 same embryo
	EMAGE:29487 same embryo
	EMAGE:29269 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source