EMAGE mouse gene expression spatial database

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EMAGE:31359

Cisd1 CDGSH iron sulfur domain 1 ( MGI:1261855)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003163_01	euxassay_003163_02	euxassay_003163_03	euxassay_003163_04	euxassay_003163_05

euxassay_003163_06	euxassay_003163_07	euxassay_003163_08	euxassay_003163_09	euxassay_003163_10

euxassay_003163_11	euxassay_003163_12	euxassay_003163_13	euxassay_003163_14	euxassay_003163_15

euxassay_003163_16	euxassay_003163_17	euxassay_003163_18	euxassay_003163_19	euxassay_003163_20

euxassay_003163_21	euxassay_003163_22	euxassay_003163_23	euxassay_003163_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

weak

regional

weak expression: see section 08 09 10 11 12 17 18

facial vii ganglion

weak

regional

weak expression: see section 04 05 21 22

vagus x ganglion

weak

regional

weak expression: see section 09 18

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 07 08 09 10 18 19 20 21 22 23

liver lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 08 18 19 20

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4911
Entity Detected:	Cisd1, CDGSH iron sulfur domain 1 ( MGI:1261855)
Sequence:	sense strand is shown >T4911 CCCACGCGTCCGCTTGCCGCGGCGCCTGCGCAGTGGCAGTGAGTGGGCCCCGAGGTCGCGTCTTGCCCAA GTCTCCGCGGTCCCCAGCGCTCGCTCGCGCGGTCCTGCCACGGCCTTCCTGCTGCCCGCGCCATGGGCCT CAGCTCCAACTCCGCTGTGCGAGTTGAGTGGATCGCGGCCGTCACCTTTGCTGCTGGCACAGCCGCTCTC GGTTACCTGGCTTACAAGAAGTTCTACGCTAAAGAGAATCGCACCAAAGCTATGGTGAATCTTCAGATCC AGAAAGACAACCCGAAGGTGGTGCATGCCTTCGACATGGAGGATCTGGGGGATAAGGCCGTGTACTGCCG ATGCTGGAGGTCTAAAAAGTTCCCCTTCTGCGATGGGGCTCACATAAAGCACAACGAAGAGACTGGCGAC AACGTAGGACCTCTGATCATCAAGAAAAAGGAAACCTAATGGACAGTTGCGAGGCTGCACCCAGCGTGTT GTGATGTCACCTGCTGATTTACGTAGAATGGCACCCAACCCACCGTCTGATTGGCCTCCCCGGTTCTAGA TGTGGTTGGTCCCTGCAAATCACAGCTCTCATATCCATGGCATCGGCCTTGCTACTGAAACATGTGGTGC ACGTTTGTTGAAAGAAGAAGAAAGGCTAAACCAACCTCGTGCTATATGGGTTATTTTGGTCTTGTAAGGA TCCGTTCCTTTAAAATAATGGTCTTAGAATATAGTTGTATCTTGAGGTTAAAGTATTAAATTATTCCAAA ATCATGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5102315 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5102315 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003163 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source