EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31367

Tcp11l2 t-complex 11 (mouse) like 2 ( MGI:2444679)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003138_01	euxassay_003138_02	euxassay_003138_03	euxassay_003138_04	euxassay_003138_05

euxassay_003138_06	euxassay_003138_07	euxassay_003138_08	euxassay_003138_09	euxassay_003138_10

euxassay_003138_11	euxassay_003138_12	euxassay_003138_13	euxassay_003138_14	euxassay_003138_15

euxassay_003138_16	euxassay_003138_17	euxassay_003138_18	euxassay_003138_19	euxassay_003138_20

euxassay_003138_21	euxassay_003138_22	euxassay_003138_23	euxassay_003138_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

hand mesenchyme

strong

regional

strong expression: see section 01 02 23 24

hindlimb digit 3 mesenchyme

strong

regional

strong expression: see section 02 03 04 05 23 24

femur

strong

regional

strong expression: see section 08 18

liver lobe

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 moderate expression: see section 01 02

hindlimb digit 5 mesenchyme

strong

regional

strong expression: see section 03 04 05 22 23

ventral grey horn

strong

regional

strong expression: see section 11 13 15

hindlimb digit 1 mesenchyme

strong

regional

strong expression: see section 03 04 23

hindlimb digit 4 mesenchyme

strong

regional

strong expression: see section 02 03 04 05 22 23 24

foot mesenchyme

strong

regional

strong expression: see section 01

hindlimb digit 2 mesenchyme

strong

regional

strong expression: see section 02 03 04 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5382
Entity Detected:	Tcp11l2, t-complex 11 (mouse) like 2 ( MGI:2444679)
Sequence:	sense strand is shown >T5382 GGGATACGCAGAGCCCCGCGACCCCCGCCACCTAGCTTTCCTCGAGTCTCTACGCGGGATCTGGGGGTTC TCTCTCTGTGTGTGTGTGTCTATCTCTCTGTCTCCGTCTCTCTGTCTCTCTGTCTCTCTGTCTCTCTTTC TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTCTCTCTCCCCCCACCCCCATTCCATCCCCAAGGTG ATAAAGGATGCCTTTCAACGGTGAGAAGCAATGTGTGAGCGAGGACCAGCAGAGTGACTCAGAGTCTTCT CGGTTCGCCGAAGGCGTGGCTTCACTCAGTGACTATGAGTGTTCCAGGCAGAGCTTCACCAGCGATTCCT CCAGCAAATCCAGCTCTCCTGCCTCAACAAGCCCTCCCCGGGGCTTAATGTTTGATGATGTGATGGCTGC AGCAAAGAACTTATCAGACATGACTCTCGCTCATGAAATAGCTGTGAATGAGAACTTCCAGCTGAAGCAA AATGCTCTCCCCGAGAACAGCCTTGCTGGTCAAGTGAAGCGTGTTGTCCACCAGGCCTTCTGGGATGTCT TGGAAGCAGATCTCAGTGCTGAGCCTCCTCAGTATGAATATGCCATCAAACTGTTTGAAGAAATCAGAGA GATTCTTCTCTCTTTCCTCACGCCTGGCGGGAACAGACTTCACAGCCAGATCTGTGAGGTTCTGGACATC GACCTCATTAGACAGCAGGCCGAGCACAGTGCTGTCGACATCCAAGGTCTGGCCAACTATGTCATCACCA CCATGGGGAAGATATGTGCTCCTGTGAGAGATGAAGACATCCGAGAGCTGAAAGCTACCACCAACATCGT GGAGATGCTGAGACAAATCTTCCGTGTCTTGGACCTCATGAGAATGGACATGATGAATTTTGTCATTAGG AATATTAGACCTCACATCCAGCATCATTTGGTGGAATATGAGAGAAACAAATTCCAGGAAGTTGTAGAAG AAACTCCAAATGCCCTCAGTCAAACCACAGAGTGGCTGAAAGAGTCCATAGATAAAGAATTGCTTTCTGA GACTGATGTAGCTCCTGCAGCTGAACATAGCTCCACGCCTAGCCTGAGCCCTCTGTTGGTGCTTAATAAC TGTTACTTGAAACTCCTTCAGTGGGATTATCAGAAAAAAGTCTTACCAGAGACACTCATGACAGATGGAC CACGGCTTCAAGAACTGTCAGAAAAGCTCAATCAATTGAAAATGACTGCCTGCGTGGCCTTAATCACCAA CAACATGGTGGGTGCTGTTACAGAAGGCCTGCCTGAACTTGCTAACAGGTTAAAAAGGATTTCAGCTGTT CTGCTTGAAGGCATGAATAAAGAGACCTTTAACTTGAAGGAAGCCCTGCATTCTATTGGTGTTCAGACTT GTGCTGAAGTTAACAAGGCCCTGGAAGAGAGAGGCTCGCCCACTTTAAATGCTGAGGTTCAAGCTAACCT TGTCGGTCAGCTCTCCAGCCTTGAAGAGAAGGACAATCCTGTCTGCACCTTGATGGATAAACGGATCCAG TTGTACATGAAAGGCCTGCTCTGTCTTCCAAGCACTCAGAAGAGCATGCCTCCTGTGCCAGGTGGCCTTG ATGTCATCCAGCGGGAGCTGGAAGTGCTTGGCTGCCAGTATGCAAACATTGTGAATCTCAACAAACAGGT GTATGGACCATTTTATGCAAATATATTCCGAAAGCTGCTCTTCCGGGATGAAGCCGTGGGGAAGATAGAT GCTTCACTTCCCACTAACTAAAGAGACGAGATGGAGATCAGCACTTGGTACATGGTCCGTTTCCACCAGT GGCATTGGAGATGTGGCCCATTCTCCGCCCTGTCCCTAGACTGCATCCGTGTAACCTGGTAATGGATGCT AGCACCACAGAGGGCCTCACACCCCTCTTGTGTTTAAAGCAGATAGTTTTAATGAAGAAAAGTAATTTGT AATGAATTATATTACCTATATAACATTGGTGTTTTCTTAAACATTTATATTGAGATTTTGTTGTTGTTGT TTTGGACAGTGTGTCTGTGTAACAGCCCTGGCTGTCCTGGAGTATATTGCACATTTCTATGTGACTTGTT CATTCAACCTGAAAGGAAATGATTTTTGTTTATATACTTGATTATACTGCAGCTGCCTGCAGGAATACAA TGTGCAAACATTTAAAAATACATTGAAAATGAAGGGCATTCTGCTTCATTTTTTGTTAAGTGGCAAAGTA AATTTGTGTCTTGGATATGGATTTAGCCAAGTACATTTATTAGGGTTTTTTAGAATCATAGCTCTTGGCA TGTTTATTTAGTTTTTACTTAAATGTTTACTTCAACTTTTGTGGCTTTCCTAACATGGTTGTTTTATAAT AAAAACTTTATTAAACAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5364434 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5364434 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003138 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source