EMAGE mouse gene expression spatial database

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EMAGE:31369

Gjd2 gap junction protein, delta 2 ( MGI:1334209)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003139_01	euxassay_003139_02	euxassay_003139_03	euxassay_003139_04	euxassay_003139_05

euxassay_003139_06	euxassay_003139_07	euxassay_003139_08	euxassay_003139_09	euxassay_003139_10

euxassay_003139_11	euxassay_003139_12	euxassay_003139_13	euxassay_003139_14	euxassay_003139_15

euxassay_003139_16	euxassay_003139_17	euxassay_003139_18	euxassay_003139_19	euxassay_003139_20

euxassay_003139_21	euxassay_003139_22	euxassay_003139_23	euxassay_003139_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

cervical ganglion

strong

regional

strong expression: see section 10 18

ventral grey horn

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

olfactory cortex marginal layer

strong

regional

strong expression: see section 10 11 12 13 15 18 19 moderate expression: see section 16 17

cervico-thoracic ganglion

strong

regional

strong expression: see section 11 12 17

thoracic ganglion

strong

regional

strong expression: see section 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5389
Entity Detected:	Gjd2, gap junction protein, delta 2 ( MGI:1334209)
Sequence:	sense strand is shown >T5389 AGCCCCGCAGCTAGCTCTGCGCGCCGCAGCGCGGGAGCGCCAGGTGCCCGCTCCAGTGACAGGCGCCGGG GTCCGTGCGCGGACCCTCCCTCAGACCGCAAGATCGCGGGGACCGCGGCGCAGGCACCGGTCTCCCGCGG GCAGGAGCTCGGCCACCTGCACGCTGCCACCCAGCCCCGCCCCTCGCCGGGAGACTGCGGGAGTCCATCT GACCCCCGGACGGCACCGAGGTGCTGTCCAGCTCTTCCGCACCGCGCCAGCAGCTCCATTGGTTGGGACG CACATAGATTGCTTCCCCCACTCCCCTCTTTTAAGTGCAATAAAGGGGGAGGGCCTCGTGGATTTTTTTA AAAAACAATTTTTGTTTGCTTCTTTAATTGGGGGCAATTCGCTCCCTGTCAGGAGGTGAGGCCCGGGGAG GGGCCAGGACCAAGAACGTGCCCGGTACTGCCCAGTCTTTGTCTGCTGCCTCCGGATGCACAGCGATGGG GGAATGGACCATCTTGGAGAGGCTGCTGGAAGCCGCGGTGCAGCAGCACTCCACTATGATTGGGAGGATC CTGTTGACTGTGGTGGTGATCTTCCGGATACTCATTGTGGCCATTGTAGGGGAGACGGTGTACGATGATG AGCAGACCATGTTTGTGTGCAACACCCTACAGCCCGGCTGTAACCAGGCCTGCTATGACCGCGCCTTTCC CATCTCCCATATACGTTACTGGGTCTTCCAGATCATAATGGTGTGCACCCCCAGTCTCTGTTTTATCACC TATTCTGTGCACCAATCCGCCAAGCAGCGAGAACGCCGGTACTCTACTGTCTTCCTAGCCCTGGACAGAG ACCCTGCTGAGTCTATAGGGGGACCTGGAGGAACTGGGGGTGGGGGCAGCGGAGGGAGCAAACGAGAAGA TAAGAAGTTGCAAAATGCCATTGTCAATGGGGTGCTGCAGAACACAGAGACCACCAGTAAGGAGACAGAA CCAGATTGCTTAGAGGTTAAAGAGCTGACTCCACATCCATCTGGGCTGCGCACAGCAGCAAGGTCCAAGC TCCGAAGACAGGAAGGTATCTCCCGCTTCTACATCATCCAAGTGGTGTTTCGAAATGCTCTGGAGATTGG GTTTCTGGTGGGCCAGTACTTTCTATATGGCTTCAGTGTTCCAGGGTTGTATGAGTGCAACCGTTACCCC TGCATCAAGGAGGTAGAATGTTATGTGTCTAGACCTACCGAGAAGACAGTCTTTCTGGTGTTCATGTTTG CTGTGAGCGGCATTTGTGTGGTGCTCAATCTGGCTGAACTTAACCATCTGGGATGGCGGAAGATCAAACT GGCTGTCCGGGGAGCCCAGGCCAAGAGGAAGTCAGTCTATGAGATACGTAACAAAGATCTGCCTCGAGTC AGTGTTCCCAATTTCGGCAGGACTCAGTCCAGTGACTCTGCCTATGTGTGAAAGGGCAGGTTTGGGGAAG GCCTGCTAGGTGGCAAGTAGCCCCAAGGCAGATAATGACTCGGGGTTTACAGGTTTGCCCTCATTCGGGT GATGCTCGCTGTGAAGGTCTAGGATACAGATATTAAGAGGTCTTCATCAACAAAGAGGAGAGGTAGAACT GTCATCACGTGTTACATCATCAGTTCTACAGAAACCATTGGGTAGAGTGACAAGATGGCTAAGCTGGACA TTCTTGGGCCTTCTTCTTTTAATGGCCCTTCCTGCTCCTGATCATAGTGAACTGGCATAGCTGCCAAGCA AGAATTAGCTCTGCTGAGCTCTGTATTCTGGTGAATGGCATGAGTCAAACACTCATTGATATAAAATTTG TGACCCATCTCAGCACATACCCTTATCCTGGGCTGCAGAAAAAAAAATCCTGGAGGATATTTCTCCCCCT TCAGCATGCCCCTTTCCCAAACCCAGGATAGCATGCCAGCTTTTCTTTTTCCTTGATCTGGCCTTACATT AGCCCTAACACAGTTTATCTGCAAGCTAGAGGGAATTACGTGGGGTGCTTTTTGGAGACTAGCCAGGGCA GAGGTTTGCAGTAGAGCCCCATATGACTGGCTCTTAGTCATTAAACAAAACACAAACCTTAGGAATGTCT CAATATTTCCAATTTTTTAAAACTCAAATTGCACTGGTGCTGTTTATTAGGGGTGGGGAATGGGAGGGGG GAATTTCAACACCACTTATGAGATGGTCATTTCACTCACCGTTGAGCATGTGTTTATGAGCTTTGGGATA TTTCTGCCTTCTAGATTATGGACTGTGGATAGAATTAAAAATATGTGGGTTTTTGTTTTGTTTTGTTGTT ATTGTTGTTGTTGTTGAAATGGAGGGTATCTACTCAAGCCACAGAATTTCTAACTGGTAGATCTTACTAT AATTTCTTACCTAATCTTGTGCTACGCAGCTGCACATCATTTTGCCATGGTTTGAACTTACCTGTGTGCT CACACCAGAGACCTTAATCAGCCCGTGTCAATCCCAACTTATTGTGTCATCAATTATTTTTTGCAATACA CACACATGCACACAATTACTGGCTTAACAAACCTGGAAGTAAATATTATTGGTTAAGATATGTTTATGGA TATTTATTTGAGACATATACCAAAGGTTCATAAACCTGTTGTTTGGACTGAGACAATTTTCCTCTCGTTG CTCTTATTCATTTAAGTGCCATATTCCTCCTTTCCTGCCCACTGTGCATTTAGCTGAAGCACTAGGCAAG AGTAGCATTGAGATAATATGTTTTTATGGAAGCCCTCTATTTCATTTGAATCCTTCTTCTTATGGGTTTG TTTTTGATTTAGATATGGATGTGGTATGTTATATTTGGATTTGTTTAAAATAAAGTTCTTGCCTTTTTTT GTAAACTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5364942 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5364942 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003139 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source