EMAGE mouse gene expression spatial database

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EMAGE:31378

Dner delta/notch-like EGF-related receptor ( MGI:2152889)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003135_01	euxassay_003135_02	euxassay_003135_03	euxassay_003135_04	euxassay_003135_05

euxassay_003135_06	euxassay_003135_07	euxassay_003135_08	euxassay_003135_09	euxassay_003135_10

euxassay_003135_11	euxassay_003135_12	euxassay_003135_13	euxassay_003135_14	euxassay_003135_15

euxassay_003135_16	euxassay_003135_17	euxassay_003135_18	euxassay_003135_19	euxassay_003135_20

euxassay_003135_21	euxassay_003135_22	euxassay_003135_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

strong

regional

strong expression: see section 01 02 03 23

extrinsic ocular muscle

moderate

regional

moderate expression: see section 02 03 04 05 06 21 22 23

head mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 21 22 23

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 10 11 12 13 15 16 17 18

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 18 19 20

tongue

moderate

regional

moderate expression: see section 11 12 13 14 15 16

spinal cord

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17

axial skeleton

moderate

regional

moderate expression: see section 12 13 14

foot mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 20 21 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 09 19 20

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 15 16 17 18 19

facial vii ganglion

strong

regional

strong expression: see section 04 21 22 moderate expression: see section 05 06

not examined

regional

not examined expression: see section 01 02 03 23

midbrain

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

vagus x ganglion

strong

regional

strong expression: see section 18

hand mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 23

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

diencephalon

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 08 09 moderate expression: see section 03 04 05 06 07 18 19 20 21 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

nucleus pulposus

moderate

regional

moderate expression: see section 12 13

telencephalon marginal layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hindbrain

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5394
Entity Detected:	Dner, delta/notch-like EGF-related receptor ( MGI:2152889)
Sequence:	sense strand is shown >T5394 GTCAGGCCGGTCCCGCTGCGGTTTCACAGATTCTGCGCCAAGAGGTCGCGAGTGGCAGCTGCGAGCCATC TGAAGCGATCTCTTCAGGCTGCCGGGGCCTCTCCATTCACTTTCTACCCCAGCATCACCTCTCTCTCCTC TCTCAGCCGACGGCTCCCACCTCTGTGCCATCCTGCCTTCCTTTGGACACACCCACGACTCTGACCCCTT AAGGCTGGGGACCGAAGACAGCCTTCTTGCAACTTGCTGAGACGCGGAGCAGAAGGGTTCCCATCCCGGG TCCTCCAGCCGGGAGCCCAGCCTCCGTGGGCCCCGCCCCCGGCCCCGCCCGCGGTTGCCCGGGGAACCAC GGGCTCCCGCAGCTGCAGGAGGCGTCCGGTCCCGCTGAGCAGCACTAGCCGCTGGCGCCGCTGCCACCGC CATCTCCGCCGCTAGCAGCGCCGCCGTCACCGCGCCCAGCTCCTCTCCAGCTTCTGCGTTCTCTCCGCTC GCTATGCCTCCCCGCCGCGCCCAGGCGCCCGGTGCCCCGCTGCTGCCCGTGCTGGCCCTGCTGCCCCTGC TGCTGGGTGCTGGGCCCCAAAGCGGCTGCTTGGCTAGCCCGGTGTCCGCCGCGCCCCTGCCTGCTCCGGG ACCGTGCGCCTCCCAGCCCTGTCGGAACGGGGGCGTGTGCACTCCGCGTTCGGTGACCGACCAAGAGCAC CCTGCGGCCGATGCTGAGCCCCGCTACAGCTGCACCTGTCCCGCTGGGGTCTCTGGCACCTATTGTCAGT TTGTTGCAGATCCTTGTGCCAGCAACCCTTGTCACCATGGTAACTGCAGCAGCAGCAGCAGCAGCAGCAG CGACAGCTACCTCTGCATTTGCAACGACGGCTATGAAGGTCTTAACTGTGAACAACCACTTCCCAGTATT CCAACCTCTGGCTGGACAGAGTCCACGGCACCCAGACAGCTTCAGCCTGTCCCCGCCACCCAGGAGCCTG ACATAATCCTGCCCCGCTCTCAGGCAACCGTGACACTGCCAACTTGGCAGCCAAAAACAGGGCAGAAAGT TGTAGAAATGAAATGGGATCAAGTAGAGGTGGTCCCTGATGTTGCCTGTGGGAATGCCAGTTCCAACAAC TCTGCGGGTGGCCGCCTAGTGTCTTTTGAAGTGCCACAGAACACCTCTGTAAAGATTCGGCAGGATGCCA ATTCTTTGCTGATCTTGCTGTGGAAGGTCACAGCCACAGGCTTTCAACAGTGTTCCCTCATAGATGGTCG AAGTGTGACACCCCTCCAGGCTCCAGGGGGCCTTGTCCTGCTAGAGGAGATGCTGGCCTTGGGACCCAAC CACTTCATTGGTTTTGTGAATGACTCTGTTGCTAAATCCATTGTGGCTTTGCGTTTAACACTTGTGGTGA AGGCTAGCAACTGTGTTCCGGGGGACAGCCACTCAAATGACTTAGAGTGTTCGGGAAAAGGAAAATGTGC CACGAAGCCATCAGAGGCAACCTTTTCCTGCACCTGCCAGGACCAGTACATTGGCACCTTCTGTGAAGAA TTTGATGCCTGCCAGAGGAAACCCTGCCAGAATGAGGCGAGCTGTATTGATGCAAATGAGAAGCAGGATG GGAGCAATTTCACTTGCCTTTGTCTTCCAGGTTATACTGGAGAGCTTTGCCAGTCCAAGATTGATTACTG TGTCCTAGACCCATGCAGAAATGGAGCCACATGTGTGTCCAGCCTCAGCGGGTTCACCTGCCAGTGTCTG GAAGGGTACTTCGGCTCCGCTTGTGAAGAGAAGGTGGACCCTTGCATGTCCTCTCCATGCCAGAACAATG GCACTTGCTACGTGGATGGCGTGCATTTCACCTGCAGCTGCAGCCCAGGCTTCACAGGGCCTACGTGTGC CCAGCTGGTGGACTTCTGCGCCCTCAGTCCGTGTGCTCATGGCATGTGCCGTAGCGTGGGCACCAGCTAC AAGTGCCTCTGCGATCCAGGTTACCATGGCCTCTACTGTGAGGAGGAATACAATGAGTGCCTGTCGGCCC CTTGCCTGAATGCCGCCACCTGCCGGGACCTCATCAATGGCTATGAGTGTGTGTGCCTGGCAGAATATAA AGGAACACACTGCGAACTGTACAAGGACCCCTGTGCTAACATCAGCTGTCTGAATGGAGGTACCTGTGAC AGCGAGGGGCTGAATGGCACGTGCATCTGTGCACCCGGGTTCACAGGTGAAGAGTGTGACATTGACATCA ATGAATGTGATAGTAACCCATGTCATCATGCTGGAACCTGCTTGGACCAGCCCAATGGTTACACCTGCCA CTGTCCTCATGGCTGGGTGGGAGCAAACTGTGAAATCCACCTGCAGTGGAAGTCTGGGCACATGGCCGAG AGCCTCACCAACATGCCTCGGCACTCCCTCTACATCATCATCGGAGCCCTCTGCGTCGCCTTCATCCTCA TGCTCATCATCCTGATTGTGGGCATCTGCCGCATCAGCCGCATCGAGTACCAGGGCTCCTCCCGGCCAGC CTATGAGGAGTTCTACAACTGCCGCAGCATTGACAGTGAGTTCAGCAATGCCATCGCATCCATCCGGCAT GCCAGGTTTGGGAAGAAGTCTCGGCCTGCCATGTACGACGTGACTCCCATTGCCTACGAGGATTACAGTC CCGATGACAAACCCTTGGTCACACTGATTAAAACAAAAGATTTGTAATCTTTTTTGGATTATTTTTCAAA AACATATGAGATATTAAGATCGTTTAAATATTTTTAAGAAAATTAAAAAGCTTAAGAGATGTAAAATGTT AGCTGCTCAAGAGTTTTCAGTAGACTATCGAGGAACTAATTTTCTGCAGCTTTTAGTTTAAAAAAACAGT ATTTTAAAAAAAACCAGAATTTGTGAAATCCGTAGACCATGTTTTAATGTACTTTCAGCTCTCAACACTG TATACTTCAACAAGGATGGGCTTTTTTGACAGTAGACCCATCATAAAACCCAGATGGACTTCTGTGGTTG TTACAGAATAAACCTTATGTAGGAAGAGTTTCTTTTGATGTGTGATGCCGGCTTTCTGAGTAGAGTTAGG AAAATAAATAGAGTATGATGCATAATTCAGTATAATCGTTACTTAATATGACGTGGCGTCCGAGATGTTT GTTTTGTGAAAAAAGAAAACTAGTTAAATTTATTATTCCTAGCCTGAATGGAATTAGCCTCTTTCTTATT CTGTGCATGGGTAACTTATTTTCTGCACTGTTCTGTTGAACTCTGAGGAAATGTCGTTCCTTCCAGTTTG TTTGAGTCATTTTTTTTGTATCAGTCCTTGAACTTGACCTTGTAAACATAAACAAGGAAAGAGTCTAGGG AGGCAAATAGCGATCAGTTTCAACCTACAGTTTTTCCTTTAAAAGTCAAGCATTTTTCTTTTTAATTGTG AGTGGACTAAATTATGTTGCAGGTGTTTCTTGCTAAGAGGTAGTGACATGTAAGAGGTGCCAGTCCCTTC TGTAGTGTTTCTTAGAGTGCACATTTATTTATACCCAAGACAATTTTTTGTGGCTGTATTTGATTGATAT GTGCTTCTTATGATTCTTGCTAATTTCAAAGAATATTGAATAAACGTTAGCAGGTCGAGGACAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5365190 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5365190 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003135 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source